ECMDB: Anaerobic dimethyl sulfoxide reductase chain B (P18776)

Identification

Name:

Anaerobic dimethyl sulfoxide reductase chain B

Synonyms:

DMSO reductase iron-sulfur subunit

Gene Name:

dmsB

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in electron carrier activity

Specific Function:

Electron transfer subunit of the terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds

Cellular Location:

Not Available

SMPDB Pathways:

Sulfur metabolism PW000922
dimethyl sulfoxide electron transfer PW001892

KEGG Pathways:

Sulfur metabolism ec00920

SMPDB Reactions:

1.0

2.0

2.0Electron

→

1.0menaquinone-8

1.0

2.0

1.0Menaquinol 8 + 1.0Dimethyl sulfoxide + 2.0Hydrogen ion + 2.0Electron → 1.0menaquinone-8 + 1.0Dimethyl sulfide + 1.0Water + 2.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Menaquinone 8

1.0

1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Trimethylamine N-Oxide → 1.0Water + 1.0Menaquinone 8 + 1.0Trimethylamine
ReactionCard

1.0

→

1.0

1.02-Demethylmenaquinol 8 + 1.0Hydrogen ion + 1.0Trimethylamine N-Oxide → 1.02-Demethylmenaquinone 8 + 1.0Water + 1.0Trimethylamine
ReactionCard

1.0

→

1.0

1.0Menaquinone 8

1.0Dimethyl sulfoxide + 1.0Menaquinol 8 → 1.0Dimethyl sulfide + 1.0Water + 1.0Menaquinone 8
ReactionCard

1.0

→

1.0

1.02-Demethylmenaquinol 8 + 1.0Dimethyl sulfoxide → 1.02-Demethylmenaquinone 8 + 1.0Dimethyl sulfide + 1.0Water
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21154	2-Demethylmenaquinol 8	MetaboCard
ECMDB21155	2-Demethylmenaquinone 8	MetaboCard
ECMDB21207	Dimethyl sulfide	MetaboCard
ECMDB21208	Dimethyl sulfoxide	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21551	Menaquinol 6	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB04102	Menaquinone	MetaboCard
ECMDB00906	Trimethylamine	MetaboCard
ECMDB04161	Trimethylamine N-Oxide	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
electron carrier activity
iron-sulfur cluster binding
metal cluster binding

Gene Properties

Blattner:

b0895

Gene Orientation

Clockwise

Centisome Percentage:

20.32

Left Sequence End

942637

Right Sequence End

943254

Gene Sequence:

>618 bp
ATGACAACCCAGTATGGATTTTTTATTGATTCCAGCCGTTGCACCGGTTGCAAAACCTGC
GAGCTGGCCTGTAAAGACTACAAAGATTTGACGCCAGAAGTCAGCTTCCGCCGCATTTAT
GAATATGCTGGCGGCGACTGGCAGGAAGATAACGGTGTCTGGCACCAGAACGTGTTTGCC
TACTATCTGTCGATTTCATGTAACCACTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCG
AGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATT
GGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCAATATAACGAAACGAAA
GGCCATATGACCAAATGCGATGGTTGTTATGACCGTGTTGCGGAGGGTAAAAAGCCGATC
TGTGTTGAATCTTGTCCGCTGCGGGCGCTGGATTTCGGCCCTATCGACGAGCTGCGTAAA
AAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAAT
ATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCA
AACCCGAAGGAGGTGTGA

Protein Properties

Pfam Domain Function:

Fer4 (PF00037 )

Protein Residues:

205

Protein Molecular Weight:

22869

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Anaerobic dimethyl sulfoxide reductase chain B
MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFA
YYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETK
GHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRKKHGDLAAVAPLPRAHFTKPN
IVIKPNANSRPTGDTTGYLANPKEV

References

External Links:

Resource	Link
Uniprot ID:	P18776
Uniprot Name:	DMSB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651422
Ecogene ID:	EG10233
Ecocyc:	EG10233
ColiBase:	b0895
Kegg Gene:	b0895
EchoBASE ID:	EB0229
CCDB:	DMSB_ECOLI
BacMap:	16128862

General Reference:

Bilous, P. T., Cole, S. T., Anderson, W. F., Weiner, J. H. (1988). "Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli." Mol Microbiol 2:785-795. Pubmed: 3062312
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Cammack, R., Weiner, J. H. (1990). "Electron paramagnetic resonance spectroscopic characterization of dimethyl sulfoxide reductase of Escherichia coli." Biochemistry 29:8410-8416. Pubmed: 2174699
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
Rothery, R. A., Weiner, J. H. (1991). "Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis." Biochemistry 30:8296-8305. Pubmed: 1653010