ECMDB: NADH-quinone oxidoreductase subunit M (P0AFE8)

Identification

Name:

NADH-quinone oxidoreductase subunit M

Synonyms:

NADH dehydrogenase I subunit M
NDH-1 subunit M
NUO13

Gene Name:

nuoM

Enzyme Class:

Biological Properties

General Function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific Function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

N-oxide electron transfer PW001889
Oxidative phosphorylation PW000919
dimethyl sulfoxide electron transfer PW001892
inner membrane transport PW000786
nitrate reduction VIII PW002092

KEGG Pathways:

Oxidative phosphorylation ec00190

KEGG Reactions:

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0Ubiquinol-8 + 1.0Acceptor ↔ 1.0Ubiquinone-1 + 1.0Reduced acceptor
ReactionCard

1.0

↔

1.0

1.0NADH + 1.0Ubiquinone-1 ↔ 1.0NAD + 1.0Ubiquinol-8
ReactionCard

SMPDB Reactions:

1.0

5.0

1.0

→

1.0

1.0NADH + 5.0Hydrogen ion + 1.0Ubiquinone-1 → 1.0Hydrogen ion + 1.0NAD + 1.0Ubiquinol-1
ReactionCard

1.0

4.0

2.0

1.0menaquinone-8

1.0

1.0Electron

4.0

1.0NADH + 4.0Hydrogen ion + 2.0Hydrogen ion + 1.0menaquinone-8 ? 1.0NAD + 1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Electron + 4.0Hydrogen ion
ReactionCard

1.0a menaquinone

4.0

1.0

→

1.0a menaquinol

1.0

1.0a menaquinone + 4.0Hydrogen ion + 1.0NADH → 1.0a menaquinol + 1.0NAD
ReactionCard

Complex Reactions:

1.0

4.0

1.0

→

1.0

3.0

1.02-Demethylmenaquinone 8 + 4.0Hydrogen ion + 1.0NADH → 1.02-Demethylmenaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

4.0

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0NADH + 1.0Ubiquinone-8 → 1.0NAD + 1.0Ubiquinol-8 + 3.0Hydrogen ion
ReactionCard

4.0

1.0Menaquinone 8

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0Menaquinone 8 + 1.0NADH → 1.0Menaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21154	2-Demethylmenaquinol 8	MetaboCard
ECMDB21155	2-Demethylmenaquinone 8	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB02434	Hydroquinone	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB23060	Quinone	MetaboCard
ECMDB21574	Ubiquinol-1	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21438	Ubiquinone-1	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard

GO Classification:

Function
catalytic activity
NADH dehydrogenase (quinone) activity
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain

Gene Properties

Blattner:

b2277

Gene Orientation

Counterclockwise

Centisome Percentage:

51.50

Left Sequence End

2389534

Right Sequence End

2391063

Gene Sequence:

>1530 bp
ATGTTACTACCCTGGCTAATATTAATTCCCTTTATTGGCGGCTTCCTGTGCTGGCAGACC
GAACGCTTTGGCGTCAAGGTGCCACGCTGGATCGCGCTGATCACCATGGGATTGACGCTG
GCGCTGTCGCTGCAACTGTGGTTGCAGGGCGGTTATTCACTGACGCAATCCGCCGGAATT
CCGCAGTGGCAGTCTGAATTCGACATGCCGTGGATCCCGCGTTTTGGTATCTCTATTCAT
CTCGCCATTGACGGGCTGTCGCTGCTGATGGTCGTGCTGACCGGTCTGCTCGGTGTGCTG
GCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTG
ATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTC
TTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAA
GCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCG
AGTGGCCTGGTGATGTTGATTGCCATCCTGGCGCTGGTTTTTGTTCACTACAATGCGACC
GGCGTCTGGACCTTCAACTATGAAGAGCTGCTGAATACGCCAATGTCCAGTGGTGTGGAA
TACCTGTTAATGCTGGGCTTCTTCATCGCCTTCGCAGTCAAAATGCCGGTGGTTCCGCTG
CATGGCTGGCTGCCGGATGCGCACTCCCAGGCTCCGACCGCCGGTTCCGTTGACCTCGCG
GGGATCTTGCTGAAAACTGCCGCTTACGGTTTGCTGCGTTTCTCCCTGCCGCTGTTCCCG
AACGCGTCGGCAGAGTTCGCGCCAATTGCTATGTGGCTGGGTGTTATCGGCATCTTCTAC
GGTGCGTGGATGGCCTTCGCCCAGACCGATATCAAACGTCTGATCGCCTACACCTCGGTT
TCCCACATGGGCTTCGTGCTGATTGCTATCTACACCGGCAGCCAGTTGGCCTACCAGGGC
GCGGTAATCCAGATGATTGCGCACGGTTTGTCGGCGGCGGGTCTGTTTATTCTTTGTGGT
CAGCTTTATGAACGTATCCATACCCGCGACATGCGCATGATGGGCGGTCTGTGGAGCAAG
ATGAAATGGCTGCCAGCACTGTCGCTGTTCTTTGCGGTGGCAACGCTTGGGATGCCTGGC
ACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTACCGGTG
ATTACCGTTATCTCTACCTTTGGGCTGGTCTTTGCATCTGTTTATTCGCTGGCGATGTTA
CATCGCGCTTACTTCGGTAAAGCGAAAAGCCAGATTGCCAGCCAGGAACTGCCAGGGATG
TCGCTGCGTGAGCTGTTTATGATCCTGTTGCTGGTGGTGCTGCTGGTACTGCTGGGCTTC
TATCCGCAGCCGATTCTGGATACCTCGCACTCCGCGATTGGCAATATCCAGCAGTGGTTT
GTTAATTCCGTTACTACTACAAGGCCGTAA

Protein Properties

Pfam Domain Function:

Oxidored_q1 (PF00361 )

Protein Residues:

509

Protein Molecular Weight:

56524

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

1-21
30-50
83-103
122-142
174-194
222-242
259-279
286-306
314-334
340-360
383-403
404-424
426-446
465-485

Protein Sequence:

>NADH-quinone oxidoreductase subunit M
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGI
PQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNL
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
QLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPV
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP

References

External Links:

Resource	Link
Uniprot ID:	P0AFE8
Uniprot Name:	NUOM_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1799637
Ecogene ID:	EG11773
Ecocyc:	EG11773
ColiBase:	b2277
Kegg Gene:	b2277
EchoBASE ID:	EB1722
CCDB:	NUOM_ECOLI
BacMap:	16130212

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Weidner, U., Geier, S., Ptock, A., Friedrich, T., Leif, H., Weiss, H. (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 233:109-122. Pubmed: 7690854
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
Zambrano, M. M., Kolter, R. (1993). "Escherichia coli mutants lacking NADH dehydrogenase I have a competitive disadvantage in stationary phase." J Bacteriol 175:5642-5647. Pubmed: 8366049