ECMDB: NADH-quinone oxidoreductase subunit K (P0AFE4)

Identification

Name:

NADH-quinone oxidoreductase subunit K

Synonyms:

NADH dehydrogenase I subunit K
NDH-1 subunit K
NUO11

Gene Name:

nuoK

Enzyme Class:

Biological Properties

General Function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific Function:

There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy- 5-methyl-6-decyl-1,4-benzoquinone) as substrates

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

N-oxide electron transfer PW001889
Oxidative phosphorylation PW000919
dimethyl sulfoxide electron transfer PW001892
inner membrane transport PW000786
nitrate reduction VIII PW002092

KEGG Pathways:

Oxidative phosphorylation ec00190

KEGG Reactions:

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0Ubiquinol-8 + 1.0Acceptor ↔ 1.0Ubiquinone-1 + 1.0Reduced acceptor
ReactionCard

1.0

↔

1.0

1.0NADH + 1.0Ubiquinone-1 ↔ 1.0NAD + 1.0Ubiquinol-8
ReactionCard

SMPDB Reactions:

1.0

5.0

1.0

→

1.0

1.0NADH + 5.0Hydrogen ion + 1.0Ubiquinone-1 → 1.0Hydrogen ion + 1.0NAD + 1.0Ubiquinol-1
ReactionCard

1.0

4.0

2.0

1.0menaquinone-8

1.0

1.0Electron

4.0

1.0NADH + 4.0Hydrogen ion + 2.0Hydrogen ion + 1.0menaquinone-8 ? 1.0NAD + 1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Electron + 4.0Hydrogen ion
ReactionCard

1.0a menaquinone

4.0

1.0

→

1.0a menaquinol

1.0

1.0a menaquinone + 4.0Hydrogen ion + 1.0NADH → 1.0a menaquinol + 1.0NAD
ReactionCard

Complex Reactions:

1.0

4.0

1.0

→

1.0

3.0

1.02-Demethylmenaquinone 8 + 4.0Hydrogen ion + 1.0NADH → 1.02-Demethylmenaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

4.0

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0NADH + 1.0Ubiquinone-8 → 1.0NAD + 1.0Ubiquinol-8 + 3.0Hydrogen ion
ReactionCard

4.0

1.0Menaquinone 8

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0Menaquinone 8 + 1.0NADH → 1.0Menaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21154	2-Demethylmenaquinol 8	MetaboCard
ECMDB21155	2-Demethylmenaquinone 8	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB02434	Hydroquinone	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB23060	Quinone	MetaboCard
ECMDB21574	Ubiquinol-1	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21438	Ubiquinone-1	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain

Gene Properties

Blattner:

b2279

Gene Orientation

Counterclockwise

Centisome Percentage:

51.58

Left Sequence End

2393065

Right Sequence End

2393367

Gene Sequence:

>303 bp
ATGATCCCCTTACAACATGGACTGATCCTCGCGGCAATCTTATTCGTTCTTGGCTTAACC
GGTCTGGTTATCCGTCGCAATCTGCTGTTTATGTTGATTGGTCTGGAAATCATGATTAAC
GCCTCCGCGCTGGCCTTCGTGGTCGCCGGAAGCTACTGGGGCCAGACCGACGGTCAGGTG
ATGTACATTCTCGCCATCAGCCTCGCGGCGGCAGAAGCGAGTATCGGCCTTGCGCTGCTG
CTGCAACTTCACCGTCGTCGCCAGAACCTGAACATCGATTCAGTAAGTGAGATGCGCGGA
TGA

Protein Properties

Pfam Domain Function:

Oxidored_q2 (PF00420 )

Protein Residues:

100

Protein Molecular Weight:

10845

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

4-24
28-48
60-80

Protein Sequence:

>NADH-quinone oxidoreductase subunit K
MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASALAFVVAGSYWGQTDGQV
MYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG

References

External Links:

Resource	Link
Uniprot ID:	P0AFE4
Uniprot Name:	NUOK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85675341
Ecogene ID:	EG12091
Ecocyc:	EG12091
ColiBase:	b2279
Kegg Gene:	b2279
EchoBASE ID:	EB2015
CCDB:	NUOK_ECOLI
BacMap:	16130214

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kao, M. C., Nakamaru-Ogiso, E., Matsuno-Yagi, A., Yagi, T. (2005). "Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli." Biochemistry 44:9545-9554. Pubmed: 15996109
Weidner, U., Geier, S., Ptock, A., Friedrich, T., Leif, H., Weiss, H. (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 233:109-122. Pubmed: 7690854
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837