NADH-quinone oxidoreductase subunit I (P0AFD6)

Identification
Name:NADH-quinone oxidoreductase subunit I
Synonyms:
  • NADH dehydrogenase I subunit I
  • NDH-1 subunit I
  • NUO9
Gene Name:nuoI
Enzyme Class:
Biological Properties
General Function:Involved in electron carrier activity
Specific Function:NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Cellular Location:Cell inner membrane; Peripheral membrane protein (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acceptor ↔ 1.0Thumb+1.0Reduced acceptor
1.0Ubiquinol-8 + 1.0Acceptor ↔ 1.0Ubiquinone-1 + 1.0Reduced acceptor
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NADH + 1.0Ubiquinone-1 ↔ 1.0NAD + 1.0Ubiquinol-8
ReactionCard
SMPDB Reactions:
1.0Thumb+5.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADH + 5.0Hydrogen ion + 1.0Ubiquinone-1 → 1.0Hydrogen ion + 1.0NAD + 1.0Ubiquinol-1
ReactionCard
1.0Thumb+4.0Thumb+2.0Thumb+1.0menaquinone-8 ? 1.0Thumb+1.0Thumb+1.0Thumb+1.0Electron+4.0Thumb
1.0NADH + 4.0Hydrogen ion + 2.0Hydrogen ion + 1.0menaquinone-8 ? 1.0NAD + 1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Electron + 4.0Hydrogen ion
ReactionCard
1.0a menaquinone+4.0Thumb+1.0Thumb → 1.0a menaquinol+1.0Thumb
1.0a menaquinone + 4.0Hydrogen ion + 1.0NADH → 1.0a menaquinol + 1.0NAD
ReactionCard
Complex Reactions:
1.0Thumb+4.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
1.02-Demethylmenaquinone 8 + 4.0Hydrogen ion + 1.0NADH → 1.02-Demethylmenaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard
4.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
4.0Hydrogen ion + 1.0NADH + 1.0Ubiquinone-8 → 1.0NAD + 1.0Ubiquinol-8 + 3.0Hydrogen ion
ReactionCard
4.0Thumb+1.0Menaquinone 8+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
4.0Hydrogen ion + 1.0Menaquinone 8 + 1.0NADH → 1.0Menaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB02434HydroquinoneMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB23060QuinoneMetaboCard
ECMDB21574Ubiquinol-1MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21438Ubiquinone-1MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
membrane
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
electron carrier activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2281
Gene OrientationCounterclockwise
Centisome Percentage:51.60
Left Sequence End2393930
Right Sequence End2394472
Gene Sequence:
>543 bp
ATGAAATTAAGATTTATTTCGTCTGCGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGT
TATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCC
TGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGAT
ATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGAC
TGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATC
AATGATAATTTATTGGCCATTGCATCCAGTACCAATACAACAACAGCAACGGGTGTCGGT
ATTGAAATACTTGATAATACATCCGCAATTCTCAAACCTGATGGGAATAGCTTCTCAACC
AACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACC
GGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAA
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:180
Protein Molecular Weight:20538
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>NADH-quinone oxidoreductase subunit I
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERC
VACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
References
External Links:
ResourceLink
Uniprot ID:P0AFD6
Uniprot Name:NUOI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674667
Ecogene ID:EG12089
Ecocyc:EG12089
ColiBase:b2281
Kegg Gene:b2281
EchoBASE ID:EB2013
CCDB:NUOI_ECOLI
BacMap:16130216
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Leif, H., Sled, V. D., Ohnishi, T., Weiss, H., Friedrich, T. (1995). "Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Eur J Biochem 230:538-548. Pubmed: 7607227
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Weidner, U., Geier, S., Ptock, A., Friedrich, T., Leif, H., Weiss, H. (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 233:109-122. Pubmed: 7690854
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837