NADH-quinone oxidoreductase subunit H (P0AFD4)

Identification
Name:NADH-quinone oxidoreductase subunit H
Synonyms:
  • NADH dehydrogenase I subunit H
  • NDH-1 subunit H
  • NUO8
Gene Name:nuoH
Enzyme Class:
Biological Properties
General Function:Involved in oxidation-reduction process
Specific Function:NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acceptor ↔ 1.0Thumb+1.0Reduced acceptor
1.0Ubiquinol-8 + 1.0Acceptor ↔ 1.0Ubiquinone-1 + 1.0Reduced acceptor
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0NADH + 1.0Ubiquinone-1 ↔ 1.0NAD + 1.0Ubiquinol-8
ReactionCard
SMPDB Reactions:
1.0Thumb+5.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADH + 5.0Hydrogen ion + 1.0Ubiquinone-1 → 1.0Hydrogen ion + 1.0NAD + 1.0Ubiquinol-1
ReactionCard
1.0Thumb+4.0Thumb+2.0Thumb+1.0menaquinone-8 ? 1.0Thumb+1.0Thumb+1.0Thumb+1.0Electron+4.0Thumb
1.0NADH + 4.0Hydrogen ion + 2.0Hydrogen ion + 1.0menaquinone-8 ? 1.0NAD + 1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Electron + 4.0Hydrogen ion
ReactionCard
1.0a menaquinone+4.0Thumb+1.0Thumb → 1.0a menaquinol+1.0Thumb
1.0a menaquinone + 4.0Hydrogen ion + 1.0NADH → 1.0a menaquinol + 1.0NAD
ReactionCard
Complex Reactions:
1.0Thumb+4.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
1.02-Demethylmenaquinone 8 + 4.0Hydrogen ion + 1.0NADH → 1.02-Demethylmenaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard
4.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
4.0Hydrogen ion + 1.0NADH + 1.0Ubiquinone-8 → 1.0NAD + 1.0Ubiquinol-8 + 3.0Hydrogen ion
ReactionCard
4.0Thumb+1.0Menaquinone 8+1.0Thumb → 1.0Thumb+1.0Thumb+3.0Thumb
4.0Hydrogen ion + 1.0Menaquinone 8 + 1.0NADH → 1.0Menaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB02434HydroquinoneMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB23060QuinoneMetaboCard
ECMDB21574Ubiquinol-1MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21438Ubiquinone-1MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
membrane
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2282
Gene OrientationCounterclockwise
Centisome Percentage:51.61
Left Sequence End2394487
Right Sequence End2395464
Gene Sequence:
>978 bp
ATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGT
CTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAG
ACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCGAT
CCTGCTGATCTGGCTTATAAAGCACTGGCGGTGAACCTAAGCGATCTGGCAGCGATGGGG
GCCGATCCGGCCTGGCTGACGCTGGCATTAACCTTACCGGACGTAGACGAAGCGTGGCTT
GAGTCCTTCAGCGACAGTTTGTTTGATCTTCTCAATTATTACGATATGCAACTCATTGGC
GGCGATACCACGCGTGGGCCATTATCAATGACGTTGGGTATCCACGGCTTTGTTCCGATG
GGACGAGCCTTAACGCGCTCTGGGGCGAAACCGGGTGACTGGATCTATGTGACCGGTACA
CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCT
AAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGG
CAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCTGACGGTTTGATTTCCGAT
CTCGGGCATATCGTGAAAGCCAGCGACTGCGGCGCACGTATTGACCTGGCATTGCTGCCG
TTTTCTGATGCGCTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCT
GGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGAT
GTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATC
GAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGAC
CATTTTGCCACGCCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:325
Protein Molecular Weight:36219
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • 11-31
  • 81-101
  • 114-134
  • 154-174
  • 186-206
  • 237-257
  • 265-285
  • 304-324
Protein Sequence:
>NADH-quinone oxidoreductase subunit H
MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQNRYGPNRVGWGGSLQL
VADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVPVSPGWVVADLNIGILFFL
MMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFLGLSLMGVVAQAGSFNMTDIV
NSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYSGMKFGLFFVG
EYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTAFFMMMFILIRASLPRPRYDQVMSF
GWKICLPLTLINLLVTAAVILWQAQ
References
External Links:
ResourceLink
Uniprot ID:P0AFD4
Uniprot Name:NUOH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674557
Ecogene ID:EG12088
Ecocyc:EG12088
ColiBase:b2282
Kegg Gene:b2282
EchoBASE ID:EB2012
CCDB:NUOH_ECOLI
BacMap:16130217
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Weidner, U., Geier, S., Ptock, A., Friedrich, T., Leif, H., Weiss, H. (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 233:109-122. Pubmed: 7690854
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837