ECMDB: NADH-quinone oxidoreductase subunit E (P0AFD1)

Identification

Name:

NADH-quinone oxidoreductase subunit E

Synonyms:

NADH dehydrogenase I subunit E
NDH-1 subunit E
NUO5

Gene Name:

nuoE

Enzyme Class:

Biological Properties

General Function:

Involved in oxidoreductase activity

Specific Function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Cellular Location:

Not Available

SMPDB Pathways:

N-oxide electron transfer PW001889
Oxidative phosphorylation PW000919
dimethyl sulfoxide electron transfer PW001892
inner membrane transport PW000786
nitrate reduction VIII PW002092

KEGG Pathways:

Oxidative phosphorylation ec00190

KEGG Reactions:

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0Ubiquinol-8 + 1.0Acceptor ↔ 1.0Ubiquinone-1 + 1.0Reduced acceptor
ReactionCard

1.0

↔

1.0

1.0NADH + 1.0Ubiquinone-1 ↔ 1.0NAD + 1.0Ubiquinol-8
ReactionCard

SMPDB Reactions:

1.0

5.0

1.0

→

1.0

1.0NADH + 5.0Hydrogen ion + 1.0Ubiquinone-1 → 1.0Hydrogen ion + 1.0NAD + 1.0Ubiquinol-1
ReactionCard

1.0

4.0

2.0

1.0menaquinone-8

1.0

1.0Electron

4.0

1.0NADH + 4.0Hydrogen ion + 2.0Hydrogen ion + 1.0menaquinone-8 ? 1.0NAD + 1.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Electron + 4.0Hydrogen ion
ReactionCard

1.0a menaquinone

4.0

1.0

→

1.0a menaquinol

1.0

1.0a menaquinone + 4.0Hydrogen ion + 1.0NADH → 1.0a menaquinol + 1.0NAD
ReactionCard

Complex Reactions:

1.0

4.0

1.0

→

1.0

3.0

1.02-Demethylmenaquinone 8 + 4.0Hydrogen ion + 1.0NADH → 1.02-Demethylmenaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

4.0

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0NADH + 1.0Ubiquinone-8 → 1.0NAD + 1.0Ubiquinol-8 + 3.0Hydrogen ion
ReactionCard

4.0

1.0Menaquinone 8

1.0

→

1.0

3.0

4.0Hydrogen ion + 1.0Menaquinone 8 + 1.0NADH → 1.0Menaquinol 8 + 1.0NAD + 3.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21154	2-Demethylmenaquinol 8	MetaboCard
ECMDB21155	2-Demethylmenaquinone 8	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB02434	Hydroquinone	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB23060	Quinone	MetaboCard
ECMDB21574	Ubiquinol-1	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21438	Ubiquinone-1	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard

GO Classification:

Function
binding
catalytic activity
NAD or NADH binding
nucleotide binding
oxidoreductase activity
Process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b2285

Gene Orientation

Counterclockwise

Centisome Percentage:

51.72

Left Sequence End

2399574

Right Sequence End

2400074

Gene Sequence:

>501 bp
GTGCTGGAAACAGAAACGGGCCGCTACTCTGACACACTGCGTTCGGCGCTGGTTTCTCTC
GATGGCGATAACGCATGGGCATTAAGCGAAAGCTGGTGTGGCACTATTCAGTGGATTTGT
CCGAGTCCGTATCGGCCTCATCATGGGCGCAAAAACTGGTTTCTGGGCATTGGGCGTTTT
ACCGCTGATGAGCAGGAACAATCGGATGCAATCCGTTATGAGACGCTGCGTTCGTCGGGG
CCGGGCGGTCAACATGTCAATAAAACCGACTCGGCGGTACGCGCCACGCATTTGGCATCC
GGTATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGA
TTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCG
CAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGG
ATGGCTTTTATCGAAGGATAA

Protein Properties

Pfam Domain Function:

Complex1_24kDa (PF01257 )

Protein Residues:

166

Protein Molecular Weight:

18590

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>NADH-quinone oxidoreductase subunit E
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAD
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQT
TFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPELLERYK

References

External Links:

Resource	Link
Uniprot ID:	P0AFD1
Uniprot Name:	NUOE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674391
Ecogene ID:	EG12086
Ecocyc:	EG12086
ColiBase:	b2285
Kegg Gene:	b2285
EchoBASE ID:	EB2010
CCDB:	NUOE_ECOLI
BacMap:	16130220

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Leif, H., Sled, V. D., Ohnishi, T., Weiss, H., Friedrich, T. (1995). "Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Eur J Biochem 230:538-548. Pubmed: 7607227
Pruss, B. M., Nelms, J. M., Park, C., Wolfe, A. J. (1994). "Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids." J Bacteriol 176:2143-2150. Pubmed: 8157582
Weidner, U., Geier, S., Ptock, A., Friedrich, T., Leif, H., Weiss, H. (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 233:109-122. Pubmed: 7690854
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837