Lipopolysaccharide export system permease protein lptF (P0AF98)

Identification
Name:Lipopolysaccharide export system permease protein lptF
Synonyms:Not Available
Gene Name:lptF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lipopolysaccharide-transporting ATPase acti
Specific Function:Part of the ABC transporter complex lptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Complex Reactions:
1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(O16 antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A diphosphate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A diphosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphoethanolamine KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Phosphoethanolamine KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0core oligosaccharide lipid A+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0core oligosaccharide lipid A + 1.0Water → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211734-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid AMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21235KDO(2)-lipid IV(A)MetaboCard
ECMDB21031KDO2-Lipid AMetaboCard
ECMDB21088LPS (1-O-antigen)MetaboCard
ECMDB21089LPS (4-O-antigen)MetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21352Phosphoethanolamine KDO(2)-lipid (A)MetaboCard
ECMDB21090Ra-LPSMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Gene Properties
Blattner:b4261
Gene OrientationClockwise
Centisome Percentage:96.65
Left Sequence End4484241
Right Sequence End4485341
Gene Sequence:
>1101 bp
ATGACCCCCGAACACCTTCCAACAGAACAGTATGAAGCGCAGTTAGCCGAAAAAGTGGTA
CGTTTGCAAAGTATGATGGCACCGTTTTCTGACCTGGTTCCGGAAGTGTTTCGCTCGCCG
GTCAGTCATTACCGGATGCGCGCGGAGTTCCGCATCTGGCACGATGGCGATGACCTGTAT
CACATCATTTTCGATCAACAAACCAAAAGCCGCATCCGCGTGGATAGCTTCCCCGCCGCC
AGTGAACTTATCAACCAGTTGATGACGGCGATGATTGCGGGTGTGCGTAATAATCCCGTT
CTGCGCCACAAGTTGTTCCAGATTGATTACCTCACTACACTGAGTAATCAGGCGGTGGTT
TCCCTGCTATACCATAAGAAGCTGGATGATGAGTGGCGTCAGGAAGCGGAGGCCCTGCGC
GATGCACTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAA
ATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATC
TACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGAATATTCAGATGCTG
GAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATTTACTGGAGCTGTACTGCGGC
AACGGTAACTTTTCATTAGCGCTGGCGCGTAATTTTGATCGGGTATTAGCCACCGAAATC
GCTAAGCCGTCGGTTGCTGCTGCGCAATACAACATCGCAGCTAACCATATTGATAACGTA
CAAATTATTCGTATGGCGGCAGAAGAATTTACTCAGGCGATGAATGGTGTGCGCGAGTTT
AACCGCCTGCAAGGGATCGACTTAAAGAGTTATCAGTGCGAAACCATTTTTGTCGACCCT
CCGCGCAGCGGTCTGGACAGTGAAACCGAGAAAATGGTGCAGGCGTATCCGCGTATTTTG
TACATCTCCTGTAACCCGGAAACGTTATGCAAGAATCTGGAAACATTAAGCCAGACGCAC
AAGGTCGAACGTCTGGCTCTGTTTGATCAGTTCCCCTACACGCACCATATGGAGTGCGGC
GTATTACTGACCGCGAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:366
Protein Molecular Weight:40357
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 16-36
  • 54-74
  • 101-121
  • 270-290
  • 296-316
  • 328-348
Protein Sequence:
>Lipopolysaccharide export system permease protein lptF
MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIPANLVLSLLGLGVPEMA
QLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAAMILAVFTAIVAAVNVMWA
GPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLFIESVDGSDFKDVFLAQIRPK
GNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTALLRDFRITDFQDYQAIIGHQAVA
LDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTVFMMALMVVPLSVVNPRQGRVLSML
PAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTVNLIYLALAIVLNLWDTVPVRRLRASF
SRKGAV
References
External Links:
ResourceLink
Uniprot ID:P0AF98
Uniprot Name:LPTF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676096
Ecogene ID:EG12535
Ecocyc:EG12535
ColiBase:b4261
Kegg Gene:b4261
EchoBASE ID:EB2424
CCDB:LPTF_ECOLI
BacMap:16132083
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ruiz, N., Gronenberg, L. S., Kahne, D., Silhavy, T. J. (2008). "Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli." Proc Natl Acad Sci U S A 105:5537-5542. Pubmed: 18375759