Identification
Name:Maltose-binding periplasmic protein
Synonyms:
  • MMBP
  • Maltodextrin-binding protein
Gene Name:malE
Enzyme Class:Not Available
Biological Properties
General Function:Involved in maltose transmembrane transporter activity
Specific Function:Involved in the high-affinity maltose membrane transport system malEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Adenosine diphosphate+1.0Thumb
1.0Maltotetraose + 1.0Adenosine triphosphate + 1.0Water → 1.0Maltotetraose + 1.0Phosphate + 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Maltotriose + 1.0Adenosine triphosphate + 1.0Water → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Maltotriose + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Adenosine diphosphate+1.0Thumb
1.0D-Maltose + 1.0Adenosine triphosphate + 1.0Water → 1.0D-Maltose + 1.0Phosphate + 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211101,4-alpha-D-glucanMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00163D-MaltoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21243MaltohexaoseMetaboCard
ECMDB21244MaltopentaoseMetaboCard
ECMDB01296MaltotetraoseMetaboCard
ECMDB01262MaltotrioseMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
alpha-glucoside transmembrane transporter activity
carbohydrate transmembrane transporter activity
glucoside transmembrane transporter activity
maltose transmembrane transporter activity
substrate-specific transmembrane transporter activity
sugar transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
alpha-glucoside transport
carbohydrate transport
establishment of localization
glucoside transport
maltose transport
transport
Gene Properties
Blattner:b4034
Gene OrientationCounterclockwise
Centisome Percentage:91.46
Left Sequence End4243252
Right Sequence End4244442
Gene Sequence:
>1191 bp
ATGACAACAAACACTGTTTCCCGCAAAGTGGCGTGGCTACGGGTCGTTACGCTGGCAGTC
GCCGCCTTCATCTTCAACACCACCGAATTTGTCCCTGTTGGCCTGCTCTCTGACATTGCG
CAAAGTTTTCACATGCAAACCGCTCAGGTCGGCATCATGTTGACCATTTACGCATGGGTA
GTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTA
CTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGC
TTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCG
ATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGT
TTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTG
GGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACC
CTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAA
AGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTG
GTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAACATTGCG
GGATTCAGCGCCAACTTTGCCACGGCATTACTGTTATTACTCGGTGGTGCGGGCATTATT
GGCAGCGTGATTTTCGGTAAACTGGGTAATCAGTATGCGTCTGCGTTGGTGAGTACGGCG
ATTGCGCTGTTGCTGGTGTGCCTGGCATTGCTGTTACCTGCGGCGAACAGTGAAATACAC
CTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAG
GTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGGCGCTATTCTCCGGC
ATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGG
TCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCA
ATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAG
Protein Properties
Pfam Domain Function:
Protein Residues:396
Protein Molecular Weight:43387
Protein Theoretical pI:5
PDB File:1LLS
Signaling Regions:
  • 1-26
Transmembrane Regions:
  • None
Protein Sequence:
>Maltose-binding periplasmic protein
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK
VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW
DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP
YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE
AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP
QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
References
External Links:
ResourceLink
Uniprot ID:P0AEX9
Uniprot Name:MALE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742501
PDB ID:1LLS
Ecogene ID:EG10554
Ecocyc:EG10554
ColiBase:b4034
Kegg Gene:b4034
EchoBASE ID:EB0549
CCDB:MALE_ECOLI
BacMap:16131860
General Reference:
  • Bedouelle, H., Hofnung, M. (1982). "A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12." Mol Gen Genet 185:82-87. Pubmed: 6283312
  • Bedouelle, H., Schmeissner, U., Hofnung, M., Rosenberg, M. (1982). "Promoters of the malEFG and malK-lamB operons in Escherichia coli K12." J Mol Biol 161:519-531. Pubmed: 6185687
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Duplay, P., Bedouelle, H., Fowler, A., Zabin, I., Saurin, W., Hofnung, M. (1984). "Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12." J Biol Chem 259:10606-10613. Pubmed: 6088507
  • Froshauer, S., Beckwith, J. (1984). "The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region." J Biol Chem 259:10896-10903. Pubmed: 6088520
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Quiocho, F. A., Spurlino, J. C., Rodseth, L. E. (1997). "Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor." Structure 5:997-1015. Pubmed: 9309217
  • Sharff, A. J., Rodseth, L. E., Spurlino, J. C., Quiocho, F. A. (1992). "Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis." Biochemistry 31:10657-10663. Pubmed: 1420181
  • Spurlino, J. C., Lu, G. Y., Quiocho, F. A. (1991). "The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis." J Biol Chem 266:5202-5219. Pubmed: 2002054
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841