1-phosphofructokinase (P0AEW9)

Identification
Name:1-phosphofructokinase
Synonyms:
  • Fructose 1-phosphate kinase
Gene Name:fruK
Enzyme Class:
Biological Properties
General Function:Involved in 1-phosphofructokinase activity
Specific Function:ATP + D-fructose 1-phosphate = ADP + D- fructose 1,6-bisphosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Fructose and mannose metabolism ec00051
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Fructose 1-phosphate ↔ 1.0ADP + 1.0beta-D-Fructose 1,6-bisphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Fructose 1,6-bisphosphate+1.0Thumb+1.0Thumb
1.0β-D-fructofuranose 1-phosphate + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0Fructose 1,6-bisphosphate + 1.0ADP + 1.0Fructose 1,6-bisphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Fructose 1-phosphate → 1.0ADP + 1.0Fructose 1,6-bisphosphate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Fructose 1-phosphate → 1.0ADP + 1.0Fructose 1,6-bisphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB20123beta-D-Fructose 1,6-bisphosphateMetaboCard
ECMDB01058Fructose 1,6-bisphosphateMetaboCard
ECMDB01076Fructose 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB24247β-D-fructofuranose 1-phosphateMetaboCard
GO Classification:
Function
1-phosphofructokinase activity
catalytic activity
phosphofructokinase activity
phosphotransferase activity, alcohol group as acceptor
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b2168
Gene OrientationCounterclockwise
Centisome Percentage:48.70
Left Sequence End2259449
Right Sequence End2260387
Gene Sequence:
>939 bp
ATGGATATCATCTTTTATCACCCAACGTTCGATACCCAATGGTGGATTGAGGCACTGCGC
AAAGCTATTCCTCAGGCAAGAGTCAGAGCATGGAAAAGCGGAGATAATGACTCTGCTGAT
TATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTG
TTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATG
CTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAG
GAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAG
CAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGC
ATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTT
CCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGA
CGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAAT
ACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCG
TATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTG
GATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCT
GAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACC
CGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAG
AGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:312
Protein Molecular Weight:33755
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>1-phosphofructokinase
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGG
FLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWER
FVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQCPCIIFDSSREALVAGLKAA
PWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIA
KPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLA
AMMARVDLQPFN
References
External Links:
ResourceLink
Uniprot ID:P0AEW9
Uniprot Name:K1PF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062597
Ecogene ID:EG10337
Ecocyc:EG10337
ColiBase:b2168
Kegg Gene:b2168
EchoBASE ID:EB0333
CCDB:K1PF_ECOLI
BacMap:16130106
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Orchard, L. M., Kornberg, H. L. (1990). "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus." Proc Biol Sci 242:87-90. Pubmed: 1981619