Bifunctional glutathionylspermidine synthetase/amidase (P0AES0)

Identification
Name:Bifunctional glutathionylspermidine synthetase/amidase
Synonyms:
  • Glutathionylspermidine synthase
  • GSP synthetase
  • Glutathione:spermidine ligase [ADP-forming]
  • Glutathionylspermidine amidase
  • Glutathionylspermidine amidohydrolase [spermidine-forming]
  • GSP amidase
Gene Name:gsp
Enzyme Class:
Biological Properties
General Function:Amino acid transport and metabolism
Specific Function:Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Glutathione + 1.0Spermidine ↔ 1.0ADP + 1.0Glutathionylspermidine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glutathionylspermidine + 1.0Water ↔ 1.0Glutathione + 1.0Spermidine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Glutathione + 1.0Spermidine ↔ 1.0ADP + 1.0Phosphate + 1.0Glutathionylspermidine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Glutathione + 1.0Spermidine ↔ 1.0ADP + 1.0Glutathionylspermidine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glutathionylspermidine + 1.0Water ↔ 1.0Glutathione + 1.0Spermidine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glutathione + 1.0Spermidine + 1.0Adenosine triphosphate → 1.0Glutathionylspermidine + 1.0ADP + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB20155GlutathionylspermidineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01257SpermidineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2988
Gene OrientationCounterclockwise
Centisome Percentage:67.56
Left Sequence End3134685
Right Sequence End3136544
Gene Sequence:
>1860 bp
ATGAGCAAAGGAACGACCAGCCAGGATGCCCCGTTCGGGACATTATTGGGCTACGCCCCA
GGTGGGGTAGCAATCTACTCTTCAGATTACAGTTCTCTCGATCCGCAGGAATACGAAGAT
GACGCCGTATTCCGTAGCTATATCGACGACGAATATATGGGCCACAAGTGGCAATGCGTT
GAATTTGCTCGCCGTTTTCTCTTTCTGAATTACGGTGTGGTCTTTACTGACGTGGGTATG
GCGTGGGAGATTTTCTCGCTGCGCTTCCTGCGAGAAGTGGTTAATGACAACATCCTGCCA
TTGCAGGCATTTCCTAACGGCTCGCCGCGTGCGCCGGTCGCGGGTGCGCTTCTTATCTGG
GATAAAGGCGGTGAATTTAAAGACACTGGCCATGTCGCCATCATTACCCAATTGCATGGC
AACAAAGTCCGTATTGCGGAACAGAACGTGATTCATTCCCCGTTGCCGCAAGGGCAACAG
TGGACGCGCGAGCTGGAGATGGTGGTCGAAAACGGCTGCTATACCCTGAAAGACACTTTT
GATGACACCACCATTCTGGGCTGGATGATCCAGACGGAAGATACTGAATACAGCTTACCG
CAGCCGGAAATTGCAGGCGAGCTGCTGAAAATCAGCGGAGCGCGCCTGGAAAACAAAGGC
CAGTTTGACGGTAAATGGCTGGATGAAAAAGATCCGCTGCAAAACGCCTATGTGCAGGCC
AACGGTCAGGTGATCAATCAGGATCCTTATCATTACTACACCATTACCGAGAGTGCCGAG
CAGGAGCTAATTAAAGCCACCAACGAGCTGCACCTGATGTATCTTCACGCAACCGACAAG
GTGCTGAAAGATGACAACCTGCTGGCGCTGTTCGACATCCCGAAAATCCTCTGGCCACGT
TTGCGTCTCTCCTGGCAGCGTCGCCGTCACCATATGATCACTGGTCGTATGGATTTCTGC
ATGGATGAGCGTGGCCTGAAGGTTTACGAGTACAACGCCGACTCCGCCTCCTGTCATACC
GAAGCGGGCTTGATCCTCGAACGTTGGGCGGAGCAGGGCTATAAAGGCAACGGCTTCAAT
CCGGCGGAAGGGCTGATTAACGAATTGGCTGGTGCCTGGAAACACAGTCGTGCACGTCCG
TTTGTCCATATCATGCAGGACAAAGATATCGAGGAAAACTATCACGCGCAGTTTATGGAG
CAGGCGCTGCACCAGGCGGGCTTTGAAACGCGTATCTTGCGTGGATTGGATGAACTGGGC
TGGGATGCTGCCGGGCAACTGATTGATGGGGAAGGGCGACTGGTTAACTGCGTGTGGAAA
ACCTGGGCGTGGGAAACCGCGTTTGATCAGATTCGTGAAGTTAGCGACCGTGAGTTTGCT
GCGGTGCCAATCCGTACCGGTCATCCGCAAAACGAAGTGCGTCTTATCGACGTATTGCTG
CGCCCGGAAGTGCTGGTCTTTGAGCCGCTGTGGACGGTGATCCCCGGCAACAAAGCGATT
CTGCCGATCCTCTGGTCGCTGTTCCCGCACCATCGTTACCTGCTGGATACCGATTTCACT
GTTAATGATGAACTGGTGAAAACAGGTTACGCAGTGAAACCGATCGCCGGTCGCTGTGGC
AGCAATATCGACCTCGTCAGCCATCATGAAGAGGTGCTGGACAAAACCAGCGGTAAATTT
GCCGAGCAGAAAAACATCTATCAGCAACTGTGGTGTTTGCCGAAAGTGGACGGTAAATAC
ATTCAGGTATGTACCTTCACCGTTGGCGGCAACTACGGTGGGACGTGTTTGCGCGGTGAT
GAATCACTGGTCATCAAAAAAGAGAGTGATATTGAACCGTTAATTGTGGTGAAAAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:619
Protein Molecular Weight:70531
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Bifunctional glutathionylspermidine synthetase/amidase
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCV
EFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIW
DKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTF
DDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQA
NGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR
LRLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILERWAEQGYKGNGFN
PAEGLINELAGAWKHSRARPFVHIMQDKDIEENYHAQFMEQALHQAGFETRILRGLDELG
WDAAGQLIDGEGRLVNCVWKTWAWETAFDQIREVSDREFAAVPIRTGHPQNEVRLIDVLL
RPEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVKTGYAVKPIAGRCG
SNIDLVSHHEEVLDKTSGKFAEQKNIYQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGD
ESLVIKKESDIEPLIVVKK
References
External Links:
ResourceLink
Uniprot ID:P0AES0
Uniprot Name:GSP_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1789361
Ecogene ID:EG12882
Ecocyc:EG12882
ColiBase:b2988
Kegg Gene:b2988
EchoBASE ID:EB2720
CCDB:GSP_ECOLI
BacMap:16130888
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bollinger, J. M. Jr, Kwon, D. S., Huisman, G. W., Kolter, R., Walsh, C. T. (1995). "Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase." J Biol Chem 270:14031-14041. Pubmed: 7775463
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553