| Identification |
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| Name: | 6-phosphofructokinase isozyme 1 |
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| Synonyms: | - 6-phosphofructokinase isozyme I
- Phosphofructokinase 1
- Phosphohexokinase 1
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| Gene Name: | pfkA |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in ATP binding |
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| Specific Function: | ATP + D-fructose 6-phosphate = ADP + D- fructose 1,6-bisphosphate |
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| Cellular Location: | Cytoplasm |
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| SMPDB Pathways: | - Galactose metabolism PW000821
- Sedoheptulose Bisphosphate Bypass PW002098
- glycerol metabolism PW000914
- glycerol metabolism II PW000915
- glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
- glycerol metabolism IV (glycerophosphoglycerol) PW000917
- glycerol metabolism V (glycerophosphoserine) PW000918
- glycolysis and pyruvate dehydrogenase PW000785
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| KEGG Pathways: | |
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| KEGG Reactions: | |
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| SMPDB Reactions: | |
1.0Fructose 6-phosphate | + | 1.0 | + | 1.0 | → | 1.0Fructose 1,6-bisphosphate | + | 1.0Adenosine diphosphate | + | 1.0 | + | 1.0 | + | 1.0 |
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1.0 | + | 1.0 | → | 1.0Sedoheptulose 1,7-bisphosphate | + | 1.0 | + | 1.0 |
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| EcoCyc Reactions: | |
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| Complex Reactions: | |
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| Metabolites: | |
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| GO Classification: | | Component |
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| 6-phosphofructokinase complex | | macromolecular complex | | protein complex | | Function |
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| 6-phosphofructokinase activity | | adenyl nucleotide binding | | adenyl ribonucleotide binding | | ATP binding | | binding | | catalytic activity | | nucleoside binding | | phosphofructokinase activity | | phosphotransferase activity, alcohol group as acceptor | | purine nucleoside binding | | transferase activity | | transferase activity, transferring phosphorus-containing groups | | Process |
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| alcohol metabolic process | | fructose 6-phosphate metabolic process | | fructose metabolic process | | glucose catabolic process | | glucose metabolic process | | glycolysis | | hexose metabolic process | | metabolic process | | monosaccharide metabolic process | | small molecule metabolic process |
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| Gene Properties |
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| Blattner: | b3916 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 88.49 |
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| Left Sequence End | 4105575 |
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| Right Sequence End | 4106537 |
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| Gene Sequence: | >963 bp
ATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCA
CAGGCGGTGAACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAG
GCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGC
GGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCC
AGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCA
AAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTG
ATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCA
CTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCG
ATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTT
AACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGC
GCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTC
GTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGAC
GTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAA
GGTGACGTTCCGGGGCTGGTGAAGGGGAATACCTATCTGACGCCGCAGCAACAAACGGCA
GAACTGACCGGACCGGTGAACAAAGCGATCATCGACACCGCGCAGTTTTTGAAAGAGCAG
GGCAAGGTCCCGGCTGTAGCGAATGATTACAGCCAGTACGTTACCTCGCGCTTCGTGCAA
TAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 320 |
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| Protein Molecular Weight: | 34842 |
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| Protein Theoretical pI: | 5 |
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| PDB File: | 2PFK |
| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >6-phosphofructokinase isozyme 1
MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLGLYEDRMVQLDRYSVSD
MINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIGGDGSYMGAMRLTEMGFPC
IGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSSHQRISVVEVMGRYCGDLTLA
AAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAIVAITEHMCDVDELAHFIEKETG
RETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLLLAGYGGRCVGIQNEQLVHHDIIDA
IENMKRPFKGDWLDCAKKLY |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Hellinga, H. W., Evans, P. R. (1985). "Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase." Eur J Biochem 149:363-373. Pubmed: 3158524
- Hellinga, H. W., Evans, P. R. (1987). "Mutations in the active site of Escherichia coli phosphofructokinase." Nature 327:437-439. Pubmed: 2953977
- Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
- Shirakihara, Y., Evans, P. R. (1988). "Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products." J Mol Biol 204:973-994. Pubmed: 2975709
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