Glucokinase (P0A6V8)

Identification
Name:Glucokinase
Synonyms:
  • Glucose kinase
Gene Name:glk
Enzyme Class:
Biological Properties
General Function:Involved in glucokinase activity
Specific Function:Not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Galactose metabolism PW000821
  • Secondary metabolites: Trehalose Biosynthesis and Metabolism PW000968
  • Starch and sucrose metabolism PW000941
  • Trehalose Degradation I (low osmolarity) PW002097
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0b-D-Glucose ↔ 1.0ADP + 1.0beta-D-Glucose 6-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0alpha-D-Glucose ↔ 1.0ADP + 1.0Glucose 6-phosphate
ReactionCard
SMPDB Reactions:
1.0Beta-D-Glucose+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0Beta-D-Glucose + 1.0Adenosine triphosphate + 1.0b-D-Glucose → 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0beta-D-Glucose 6-phosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0D-Glucose + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0beta-D-Glucose 6-phosphate + 1.0ADP
ReactionCard
1.0Beta-D-Glucopyranuronic acid+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Beta-D-Glucopyranuronic acid + 1.0Adenosine triphosphate → 1.0Glucose 6-phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0b-D-Glucose + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Glucose 6-phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Glucose → 1.0ADP + 1.0Glucose 6-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Glucose → 1.0ADP + 1.0Glucose 6-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB23064Alpha-D-glucose 6-phosphateMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB03498beta-D-Glucose 6-phosphateMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
glucokinase activity
hexokinase activity
nucleoside binding
phosphotransferase activity, alcohol group as acceptor
purine nucleoside binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b2388
Gene OrientationCounterclockwise
Centisome Percentage:54.02
Left Sequence End2506483
Right Sequence End2507448
Gene Sequence:
>966 bp
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATG
AGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAG
AAAATGCTGGAAGCGGGCGAAAGTGCGCTGGATGTGGTGACGGAAGCGGTGCGTCTGCTG
GAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCTGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTT
AGTCATCTGCGTAATCCGGTTCTTGCCGCCCGGCTGGTGATGGAGCAAAGCCCGCATGTG
ATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCG
CCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGG
GCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTG
GGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATG
ACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCC
AATAACGCCAGTGTGGCGGTTTCTTGTACCGGCACGGGCGAAGTCTTCATCCGCGCGCTG
GCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGC
GAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATC
GACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGC
TACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACA
CAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:321
Protein Molecular Weight:34723
Protein Theoretical pI:7
PDB File:1Q18
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucokinase
MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEVKDGC
IAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQFG
GAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGHVDFAPNSEEEAIILEILRAE
IGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLFCVI
MGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIV
HDNPGLLGSGAHLRQTLGHIL
References
External Links:
ResourceLink
Uniprot ID:P0A6V8
Uniprot Name:GLK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062400
PDB ID:1Q18
Ecogene ID:EG12957
Ecocyc:EG12957
ColiBase:b2388
Kegg Gene:b2388
EchoBASE ID:EB2791
CCDB:GLK_ECOLI
BacMap:16130320
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Meyer, D., Schneider-Fresenius, C., Horlacher, R., Peist, R., Boos, W. (1997). "Molecular characterization of glucokinase from Escherichia coli K-12." J Bacteriol 179:1298-1306. Pubmed: 9023215
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837