6-phosphofructokinase isozyme 2 (P06999)

Identification
Name:6-phosphofructokinase isozyme 2
Synonyms:
  • Phosphofructokinase-2
Gene Name:pfkB
Enzyme Class:
Biological Properties
General Function:Involved in phosphotransferase activity, alcohol group as acceptor
Specific Function:ATP + D-fructose 6-phosphate = ADP + D- fructose 1,6-bisphosphate
Cellular Location:Not Available
SMPDB Pathways:
  • Galactitol and galactonate degradation PW000820
  • Galactose metabolism PW000821
  • Sedoheptulose Bisphosphate Bypass PW002098
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • glycolysis and pyruvate dehydrogenase PW000785
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Tagatose 6-phosphate → 1.0ADP + 1.0Hydrogen ion + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Tagatose 6-phosphate + 1.0Adenosine triphosphate ↔ 1.0D-Tagatose 1,6-bisphosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.0D-Tagatose 6-phosphate ↔ 1.0CDP + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uridine triphosphate + 1.0D-Tagatose 6-phosphate ↔ 1.0Uridine 5'-diphosphate + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Inosine triphosphate + 1.0D-Tagatose 6-phosphate ↔ 1.0IDP + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0beta-D-Fructose 6-phosphate ↔ 1.0ADP + 1.0beta-D-Fructose 1,6-bisphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Fructose 6-phosphate ↔ 1.0ADP + 1.0Fructose 1,6-bisphosphate
ReactionCard
SMPDB Reactions:
1.0Fructose 6-phosphate+1.0Thumb+1.0Thumb → 1.0Fructose 1,6-bisphosphate+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0Fructose 6-phosphate + 1.0Adenosine triphosphate + 1.0Fructose 6-phosphate → 1.0Fructose 1,6-bisphosphate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0Fructose 1,6-bisphosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0D-tagatofuranose 1,6-bisphosphate+1.0Thumb
1.0D-tagatofuranose 6-phosphate + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0D-tagatofuranose 1,6-bisphosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Sedoheptulose 1,7-bisphosphate+1.0Thumb+1.0Thumb
1.0D-Sedoheptulose 7-phosphate + 1.0Adenosine triphosphate → 1.0Sedoheptulose 1,7-bisphosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Fructose 6-phosphate → 1.0ADP + 1.0Fructose 1,6-bisphosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Tagatose 6-phosphate → 1.0ADP + 1.0Hydrogen ion + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB20123beta-D-Fructose 1,6-bisphosphateMetaboCard
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB01546CDPMetaboCard
ECMDB00082Cytidine triphosphateMetaboCard
ECMDB01068D-Sedoheptulose 7-phosphateMetaboCard
ECMDB24205D-tagatofuranose 6-phosphateMetaboCard
ECMDB06872D-Tagatose 1,6-bisphosphateMetaboCard
ECMDB06873D-Tagatose 6-phosphateMetaboCard
ECMDB01058Fructose 1,6-bisphosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03335IDPMetaboCard
ECMDB00189Inosine triphosphateMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00285Uridine triphosphateMetaboCard
GO Classification:
Function
catalytic activity
phosphotransferase activity, alcohol group as acceptor
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b1723
Gene OrientationClockwise
Centisome Percentage:38.89
Left Sequence End1804394
Right Sequence End1805323
Gene Sequence:
>930 bp
ATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCT
GTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATC
GACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTC
TCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAT
CGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTC
AGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGG
AAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTG
CCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTG
AAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTC
GCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTC
TGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCG
GCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGG
GACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAG
CTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTC
TCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAA
ACCCTGCTGCAACAGCTCAAGCTTTGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:309
Protein Molecular Weight:32456
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>6-phosphofructokinase isozyme 2
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFP
AGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQ
LEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGN
IELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDT
QKIYAYLSR
References
External Links:
ResourceLink
Uniprot ID:P06999
Uniprot Name:K6PF2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674412
Ecogene ID:EG10700
Ecocyc:EG10700
ColiBase:b1723
Kegg Gene:b1723
EchoBASE ID:EB0694
CCDB:K6PF2_ECOLI
BacMap:49176138
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daldal, F. (1983). "Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme." J Mol Biol 168:285-305. Pubmed: 6310120
  • Daldal, F. (1984). "Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12." Gene 28:337-342. Pubmed: 6235149
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553