Concentrations
| ECMDB ID | Name | Concentration | Strain Media | Growth Status Growth System | Temperature | Citation |
|---|---|---|---|---|---|---|
| ECMDB21221 | Glutathione disulfide | 3670± 0 uM | BW25113 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco | Stationary Phase, glucose limited Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h | 37 oC | PMID: 17379776 |
| ECMDB00902 | NAD | 3550± 219 uM | K12 0.2 g/L NH4Cl, 2.0 g/L (NH4)2SO4, 3.25 g/L KH2PO4, 2.5 g/L K2HPO4, 1.5 g/L NaH2PO4, 0.5 g/L MgSO4; trace substances: 10 mg/L CaCl2, 0.5 mg/L ZnSO4, 0.25 mg/L CuCl2, 0.25 mg/L MnSO4, 0.175 mg/L CoCl2, 0.125 mg/L H3BO3, 2.5 mg/L AlCl3, 0.5 mg/L Na2MoO4, 10 | Stationary Phase, glucose limited Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h | 37 oC | PMID: 11488613 |
| ECMDB00156 | L-Malic acid | 3450± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00156 | L-Malic acid | 3330± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB20123 | beta-D-Fructose 1,6-bisphosphate | 3290± 179 uM | K12 0.2 g/L NH4Cl, 2.0 g/L (NH4)2SO4, 3.25 g/L KH2PO4, 2.5 g/L K2HPO4, 1.5 g/L NaH2PO4, 0.5 g/L MgSO4; trace substances: 10 mg/L CaCl2, 0.5 mg/L ZnSO4, 0.25 mg/L CuCl2, 0.25 mg/L MnSO4, 0.175 mg/L CoCl2, 0.125 mg/L H3BO3, 2.5 mg/L AlCl3, 0.5 mg/L Na2MoO4, 10 | Stationary Phase, glucose limited Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h | 37 oC | PMID: 11488613 |
| ECMDB01414 | Putrescine | 3060± 0 uM | K12 HB101 199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3 | Stationary Phase Shake flask | 37 oC | PMID: 8742354 |
| ECMDB00641 | L-Glutamine | 3060± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00082 | Cytidine triphosphate | 2730± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB01257 | Spermidine | 2720± 0 uM | K12 HB101 199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3 | Mid Log Phase Shake flask | 37 oC | PMID: 8742354 |
| ECMDB01273 | Guanosine triphosphate | 2690± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00161 | L-Alanine | 2550± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00902 | NAD | 2550± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00045 | Adenosine monophosphate | 2510± 183 uM | K12 0.2 g/L NH4Cl, 2.0 g/L (NH4)2SO4, 3.25 g/L KH2PO4, 2.5 g/L K2HPO4, 1.5 g/L NaH2PO4, 0.5 g/L MgSO4; trace substances: 10 mg/L CaCl2, 0.5 mg/L ZnSO4, 0.25 mg/L CuCl2, 0.25 mg/L MnSO4, 0.175 mg/L CoCl2, 0.125 mg/L H3BO3, 2.5 mg/L AlCl3, 0.5 mg/L Na2MoO4, 10 | Stationary Phase, glucose limited Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h | 37 oC | PMID: 11488613 |
| ECMDB04171 | UDP-Glucose | 2500± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00902 | NAD | 2430± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00290 | Uridine diphosphate-N-acetylglucosamine | 2400± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB21221 | Glutathione disulfide | 2370± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00285 | Uridine triphosphate | 2370± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00094 | Citric acid | 2320± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00883 | L-Valine | 2290± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB00296 | Uridine | 2090± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB01414 | Putrescine | 2040± 0 uM | K12 HB101 199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3 | Mid Log Phase Shake flask | 37 oC | PMID: 8742354 |
| ECMDB00094 | Citric acid | 1960± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |
| ECMDB04171 | UDP-Glucose | 1910± 0 uM | BW25113 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco | Stationary Phase, glucose limited Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h | 37 oC | PMID: 17379776 |
| ECMDB00295 | Uridine 5'-diphosphate | 1790± 0 uM | K12 NCM3722 Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose | Mid-Log Phase Shake flask and filter culture | 37 oC | PMID: 19561621 |