ECMDB: Acetyl-CoA acetyltransferase (P76461)

Identification

Name:

Acetyl-CoA acetyltransferase

Synonyms:

Acetoacetyl-CoA thiolase

Gene Name:

atoB

Enzyme Class:

2.3.1.9

Biological Properties

General Function:

Involved in transferase activity, transferring acyl groups other than amino-acyl groups

Specific Function:

2 acetyl-CoA = CoA + acetoacetyl-CoA

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Acetate metabolism PW002090
Fatty acid metabolism PW000796
Propanoate metabolism PW000940
Tryptophan metabolism PW000815

KEGG Pathways:

Benzoate degradation via CoA ligation ec00632
Benzoate degradation via hydroxylation ec00362
Butanoate metabolism ec00650
Fatty acid metabolism ec00071
Glyoxylate and dicarboxylate metabolism ec00630
Lysine degradation ec00310
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Propanoate metabolism ec00640
Pyruvate metabolism ec00620
Reductive carboxylate cycle (CO2 fixation) ec00720
Synthesis and degradation of ketone bodies ec00072
Terpenoid backbone biosynthesis ec00900
Tryptophan metabolism ec00380
Valine, leucine and isoleucine degradation ec00280

KEGG Reactions:

2.0

↔

1.0

2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard

1.0

1.0Butanoyl-CoA

↔

1.0

1.0Acetyl-CoA + 1.0Butanoyl-CoA ↔ 1.0Coenzyme A + 1.03-Oxohexanoyl-CoA
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Acetoacetyl-CoA → 1.0Coenzyme A + 1.0Acetyl-CoA
ReactionCard

EcoCyc Reactions:

2.0

↔

1.0

2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB03943	3-Oxohexanoyl-CoA	MetaboCard
ECMDB01484	Acetoacetyl-CoA	MetaboCard
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01088	Butyryl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard

GO Classification:

Function
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process

Gene Properties

Blattner:

b2224

Gene Orientation

Clockwise

Centisome Percentage:

50.09

Left Sequence End

2324131

Right Sequence End

2325315

Gene Sequence:

>1185 bp
ATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTATCGGTAGTTTTAACGGTTCA
CTCGCTTCCACCAGCGCCATCGACCTGGGGGCGACAGTAATTAAAGCCGCCATTGAACGT
GCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGG
CTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGC
GGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAG
GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTA
GCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT
TATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATT
ACCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCG
CTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATC
GTCCCGGTAAATGTTGTCACTCGAAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCG
AAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGA
ACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATG
GAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCC
AGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCG
TTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT
GCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTC
AACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTC
ACACTATTACATGCCATGCAGGCACGCGATAAAACGCTGGGGCTGGCAACACTGTGCATT
GGCGGCGGTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAA

Protein Properties

Pfam Domain Function:

Thiolase_C (PF02803 )
Thiolase_N (PF00108 )

Protein Residues:

394

Protein Molecular Weight:

40352

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Acetyl-CoA acetyltransferase
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAG
LGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSL
APYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGITAENVAKEYGITREMQDELA
LHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKA
LQLAGLQLADIDLIEANEAFAAQFLAVGKNLGFDSEKVNVNGGAIALGHPIGASGARILV
TLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN

References

External Links:

Resource	Link
Uniprot ID:	P76461
Uniprot Name:	ATOB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1736879
Ecogene ID:	EG11672
Ecocyc:	EG11672
ColiBase:	b2224
Kegg Gene:	b2224
EchoBASE ID:	EB1623
CCDB:	ATOB_ECOLI
BacMap:	16130161

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040