Imidazole glycerol phosphate synthase subunit hisF (P60664)

Identification
Name:Imidazole glycerol phosphate synthase subunit hisF
Synonyms:
  • IGP synthase cyclase subunit
  • IGP synthase subunit hisF
  • ImGP synthase subunit hisF
  • IGPS subunit hisF
Gene Name:hisF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in catalytic activity
Specific Function:IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Phosphoribulosylformimino-AICAR-P + 1.0L-Glutamine ↔ 1.0D-Erythro-imidazole-glycerol-phosphate + 1.0AICAR + 1.0L-Glutamate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0L-Glutamic acid+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Phosphoribulosylformimino-AICAR-P + 1.0L-Glutamine → 1.0L-Glutamic acid + 1.0Hydrogen ion + 1.05-Amino-4-imidazolecarboxyamide + 1.0D-Erythro-imidazole-glycerol-phosphate + 1.0L-Glutamate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Phosphoribulosylformimino-AICAR-P + 1.0L-Glutamine ↔ 1.0D-Erythro-imidazole-glycerol-phosphate + 1.0AICAR + 1.0L-Glutamate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Glutamine + 1.0Phosphoribulosylformimino-AICAR-P → 1.0Phosphoribosyl formamidocarboxamide + 1.0D-Erythro-imidazole-glycerol-phosphate + 1.0L-Glutamate + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200985-Amino-4-imidazolecarboxyamideMetaboCard
ECMDB01517AICARMetaboCard
ECMDB12208D-Erythro-imidazole-glycerol-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB00641L-GlutamineMetaboCard
ECMDB01317N5-Carboxyaminoimidazole ribonucleotideMetaboCard
ECMDB01439Phosphoribosyl formamidocarboxamideMetaboCard
ECMDB12278Phosphoribulosylformimino-AICAR-PMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
imidazoleglycerol-phosphate synthase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
histidine biosynthetic process
histidine family amino acid metabolic process
histidine metabolic process
metabolic process
Gene Properties
Blattner:b2025
Gene OrientationClockwise
Centisome Percentage:45.13
Left Sequence End2093868
Right Sequence End2094644
Gene Sequence:
>777 bp
ATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGT
TTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTAC
GACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAAT
TTACCGGCGGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAA
GAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAA
AAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCA
ACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTA
CTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAG
GCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTAT
CATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATT
GGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAAT
TATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAA
TATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:258
Protein Molecular Weight:28454
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Imidazole glycerol phosphate synthase subunit hisF
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEIVLNMMN
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC
References
External Links:
ResourceLink
Uniprot ID:P60664
Uniprot Name:HIS6_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674646
Ecogene ID:EG10448
Ecocyc:EG10448
ColiBase:b2025
Kegg Gene:b2025
EchoBASE ID:EB0443
CCDB:HIS6_ECOLI
BacMap:16129966
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carlomagno, M. S., Chiariotti, L., Alifano, P., Nappo, A. G., Bruni, C. B. (1988). "Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons." J Mol Biol 203:585-606. Pubmed: 3062174
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Kido, N., Torgov, V. I., Sugiyama, T., Uchiya, K., Sugihara, H., Komatsu, T., Kato, N., Jann, K. (1995). "Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system." J Bacteriol 177:2178-2187. Pubmed: 7536735
  • Klem, T. J., Chen, Y., Davisson, V. J. (2001). "Subunit interactions and glutamine utilization by Escherichia coli imidazole glycerol phosphate synthase." J Bacteriol 183:989-996. Pubmed: 11208798
  • Klem, T. J., Davisson, V. J. (1993). "Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis." Biochemistry 32:5177-5186. Pubmed: 8494895