ECMDB: Xanthosine phosphorylase (P45563)

Identification

Name:

Xanthosine phosphorylase

Synonyms:

Not Available

Gene Name:

xapA

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in purine-nucleoside phosphorylase activity

Specific Function:

The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except adenosine and deoxyadenosine

Cellular Location:

Not Available

SMPDB Pathways:

purine ribonucleosides degradation PW002076

KEGG Pathways:

Purine metabolism ec00230

KEGG Reactions:

1.0

↔

1.0

1.0Deoxyinosine + 1.0Phosphate ↔ 1.0Deoxyribose 1-phosphate + 1.0Hypoxanthine
ReactionCard

1.0

↔

1.0

1.0Deoxyguanosine + 1.0Phosphate ↔ 1.0Deoxyribose 1-phosphate + 1.0Guanine
ReactionCard

1.0

↔

1.0

1.0alpha-D-Ribose 1-phosphate

1.0Xanthosine + 1.0Phosphate ↔ 1.0Xanthine + 1.0alpha-D-Ribose 1-phosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Guanosine + 1.0Phosphate ↔ 1.0Guanine + 1.0Ribose-1-phosphate
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1.0

↔

1.0

1.0Inosine + 1.0Phosphate ↔ 1.0Hypoxanthine + 1.0Ribose-1-phosphate
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1.0

↔

1.0

1.0Phosphate + 1.0Xanthosine ↔ 1.0Ribose-1-phosphate + 1.0Xanthine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00085	Deoxyguanosine	MetaboCard
ECMDB00071	Deoxyinosine	MetaboCard
ECMDB01351	Deoxyribose 1-phosphate	MetaboCard
ECMDB00132	Guanine	MetaboCard
ECMDB00133	Guanosine	MetaboCard
ECMDB00157	Hypoxanthine	MetaboCard
ECMDB00195	Inosine	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB04147	Ribose-1-phosphate	MetaboCard
ECMDB00292	Xanthine	MetaboCard
ECMDB00299	Xanthosine	MetaboCard

GO Classification:

Function
catalytic activity
purine-nucleoside phosphorylase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process

Gene Properties

Blattner:

b2407

Gene Orientation

Counterclockwise

Centisome Percentage:

54.36

Left Sequence End

2522067

Right Sequence End

2522900

Gene Sequence:

>834 bp
ATGCATGTTTTTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAG
GATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTAC
AATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTA
GTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATC
CATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCCGCGATATACGCTTGAAGATG
TGTGGTTATCAGGCTTATTTTAGTCGTACGGGGAGAAAGGAAATTCCTTCCGGCAATAGA
ACGAAACGAATATTGATAAATGTTCCAGGTATTTATAGTGACAGTTTTTGGGCGTCTATA
ATACGTGGAGAACTATCAGAGCTTTCACAGCCTACAGATGATGAATCGCTTCTGAATATG
AGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTT
GAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTA
CAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTAAATGAT
GGAAACCCATATTTGGAAGTACATCATGTAATTCCCCTGTCTTCAGGTGGTGCTGATACA
ACAGATAACTGTGTTGCCCTTTGTCCGAATTGCCATAGAGAATTGCACTATAGTAAAAAT
GCAAAAGAACTAATCGAGATGCTTTACGTTAATATAAACCGATTACAGAAATAA

Protein Properties

Pfam Domain Function:

PNP_UDP_1 (PF01048 )

Protein Residues:

277

Protein Molecular Weight:

29834

Protein Theoretical pI:

PDB File:

1YR3

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Xanthosine phosphorylase
MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIENAVAISYEKLPGFPVS
TVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGS
LRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKEEG
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITNM
AEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKIA

References

External Links:

Resource	Link
Uniprot ID:	P45563
Uniprot Name:	XAPA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651571
PDB ID:	1YR3
Ecogene ID:	EG20250
Ecocyc:	EG20250
ColiBase:	b2407
Kegg Gene:	b2407
EchoBASE ID:	EB4152
CCDB:	XAPA_ECOLI
BacMap:	16130333

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Seeger, C., Poulsen, C., Dandanell, G. (1995). "Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli." J Bacteriol 177:5506-5516. Pubmed: 7559336
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837