ECMDB: Ferric iron reductase protein fhuF (P39405)

Identification

Name:

Ferric iron reductase protein fhuF

Synonyms:

Not Available

Gene Name:

fhuF

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in 2 iron, 2 sulfur cluster binding

Specific Function:

Involved in the reduction of ferric iron in cytoplasmic ferrioxamine B

Cellular Location:

Cytoplasm. Cell membrane; Peripheral membrane protein

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

1.0

2.0

→

1.0

2.0

1.0FADH2 + 2.0Ferroxamine → 1.0FAD + 2.0Iron + 2.0ferroxamine minus Fe(3) + 2.0Hydrogen ion
ReactionCard

2.0

1.0

→

2.0

1.0

2.0

2.0Ferroxamine + 1.0FMNH → 2.0Iron + 2.0ferroxamine minus Fe(3) + 1.0Flavin Mononucleotide + 2.0Hydrogen ion
ReactionCard

2.0

1.0

→

2.0

1.0

2.0Ferroxamine + 1.0Reduced riboflavin → 2.0Iron + 2.0ferroxamine minus Fe(3) + 2.0Hydrogen ion + 1.0Riboflavin
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01248	FAD	MetaboCard
ECMDB01197	FADH2	MetaboCard
ECMDB21333	Ferroxamine	MetaboCard
ECMDB21332	ferroxamine minus Fe(3)	MetaboCard
ECMDB01520	Flavin Mononucleotide	MetaboCard
ECMDB01142	FMNH	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00692	Iron	MetaboCard
ECMDB21279	Reduced riboflavin	MetaboCard
ECMDB00244	Riboflavin	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b4367

Gene Orientation

Counterclockwise

Centisome Percentage:

99.21

Left Sequence End

4602898

Right Sequence End

4603686

Gene Sequence:

>789 bp
GTGATTGATAAATCCGCCTTTGTGCATCCAACCGCCATTGTGGAAGAGGGCGCGTCAATT
GGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAG
GGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAAT
GAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAA
CCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGT
GGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAC
GCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACG
CTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCAT
CAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGAC
GTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAA
GGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAG
CTGATTTATCGTAGCGGTAAAACGCTCGATGAAGTGAAACCGGAAATTGCTGAACTGGCG
GAAACATATCCGGAAGTGAAAGCCTTTACCGATTTCTTTGCACGCTCAACGCGCGGTCTG
ATTCGTTAA

Protein Properties

Pfam Domain Function:

FhuF (PF06276 )

Protein Residues:

262

Protein Molecular Weight:

30113

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Ferric iron reductase protein fhuF
MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFIRLDEPAPLNAMTLAQW
SSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGLMVPPLMLALLTQEKALDV
SPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQHRMETLISQALVPVVQALEATGEING
KLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTNGEDNPLWRTVVLRDGLLV
RRTCCQRYRLPDVQQCGDCTLK

References

External Links:

Resource	Link
Uniprot ID:	P39405
Uniprot Name:	FHUF_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674370
Ecogene ID:	EG12595
Ecocyc:	EG12595
ColiBase:	b4367
Kegg Gene:	b4367
EchoBASE ID:	EB2480
CCDB:	FHUF_ECOLI
BacMap:	16132188

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Muller, K., Matzanke, B. F., Schunemann, V., Trautwein, A. X., Hantke, K. (1998). "FhuF, an iron-regulated protein of Escherichia coli with a new type of [2Fe-2S] center." Eur J Biochem 258:1001-1008. Pubmed: 9990318