L-idonate 5-dehydrogenase (P39346)

Identification
Name:L-idonate 5-dehydrogenase
Synonyms:Not Available
Gene Name:idnD
Enzyme Class:
Biological Properties
General Function:Involved in zinc ion binding
Specific Function:Catalyzes the NADH/NADPH-dependent oxidation of L- idonate to 5-ketogluconate (5KG)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.05-Dehydro-D-gluconate+1.0Thumb+1.0Thumb
1.0L-Idonate + 1.0NADP ↔ 1.05-Dehydro-D-gluconate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Idonate + 1.0NAD + 1.0NADP ↔ 1.05-Keto-D-gluconate + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0NADPH+1.05-Keto-D-gluconate+1.0Thumb+1.0Thumb
1.0L-Idonate + 1.0NADP → 1.0Hydrogen ion + 1.0NADPH + 1.05-Keto-D-gluconate + 1.0NADPH + 1.05-Keto-D-gluconate
ReactionCard
EcoCyc Reactions:
1.0NAD(P)++1.0Thumb ↔ 1.0NAD(P)H+1.0Thumb+1.0Thumb
1.0NAD(P)+ + 1.0L-Idonate ↔ 1.0NAD(P)H + 1.05-Keto-D-gluconate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Keto-D-gluconate + 1.0Hydrogen ion + 1.0NADH ↔ 1.0D-Galactonate + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.05-Keto-D-gluconate + 1.0Hydrogen ion + 1.0NADPH → 1.0D-Galactonate + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Galactonate + 1.0NAD → 1.0Hydrogen ion + 1.0NADH + 1.05-Keto-D-gluconate
ReactionCard
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.0L-Idonate + 1.0NAD(P)(+) → 1.05-Keto-D-gluconate + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB117315-Keto-D-gluconateMetaboCard
ECMDB20140D-GalactonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21376L-IdonateMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
metal ion binding
oxidoreductase activity
transition metal ion binding
zinc ion binding
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b4267
Gene OrientationCounterclockwise
Centisome Percentage:96.80
Left Sequence End4491398
Right Sequence End4492429
Gene Sequence:
>1032 bp
ATGGCGACCATAAAGGATGTAGCCCGACTGGCAGGCGTTTCAGTCGCCACCGTTTCCCGC
GTCATTAATAATTCACCCAAAGCCAGCGAAGCTTCCCGGCTGGCTGTGCATAGTGCAATG
GAGTCTCTTAGCTATCACCCGAACGCCAACGCCCGTGCGCTGGCGCAGCAGACCACTGAA
ACGGTCGGTCTGGTCGTTGGTGATGTTTCCGATCCGTTTTTCGGTGCAATGGTGAAAGCG
GTCGAACAGGTGGCTTATCACACCGGTAATTTTTTATTGATTGGCAACGGTTACCACAAC
GAACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTC
GTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGT
ATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGAT
CGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGT
TATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGAT
GCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGACATTTGGCGAACCAGAC
GAAAGCGGCGGCGAACAGGCAATGACCGAGCTTTTGGGACGAGGAAGAAATTTCACTGCG
GTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGT
ATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACGCTAT
GTGCGTCCGCGCCTGACCACCGTGCGTTACCCAATCGTGACGATGGCGACCCAGGCTGCC
GAACTGGCTTTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGT
CCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCA
ACCAGCGACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:343
Protein Molecular Weight:37146
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>L-idonate 5-dehydrogenase
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAP
MVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEHNENQCTDMRFFGSAMYFP
HVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIHAAHQAGELQGKRVFISGVGP
IGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAV
SWLANGVINPLPLLSAEYPFTDLEEALRFAGDKTQAAKVQLVF
References
External Links:
ResourceLink
Uniprot ID:P39346
Uniprot Name:IDND_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675653
Ecogene ID:EG12541
Ecocyc:EG12541
ColiBase:b4267
Kegg Gene:b4267
EchoBASE ID:EB2430
CCDB:IDND_ECOLI
BacMap:16132089
General Reference:
  • Bausch, C., Peekhaus, N., Utz, C., Blais, T., Murray, E., Lowary, T., Conway, T. (1998). "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli." J Bacteriol 180:3704-3710. Pubmed: 9658018
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553