Nitric oxide reductase FlRd-NAD(+) reductase (P37596)

Identification
Name:Nitric oxide reductase FlRd-NAD(+) reductase
Synonyms:
  • Flavorubredoxin reductase
  • FlRd-reductase
  • FlavoRb reductase
Gene Name:norW
Enzyme Class:Not Available
Biological Properties
General Function:Involved in oxidation-reduction process
Specific Function:One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADH + 2.0Nitric oxide → 1.0Water + 1.0Nitrous oxide + 1.0NAD
ReactionCard
1.0Reduced NO reductase rubredoxin+1.0Thumb → 1.0oxidized NO reductase rubredoxin+1.0Thumb
1.0Reduced NO reductase rubredoxin + 1.0NAD → 1.0oxidized NO reductase rubredoxin + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB03378Nitric oxideMetaboCard
ECMDB21255Nitrous oxideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
nucleoside binding
oxidoreductase activity
purine nucleoside binding
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2711
Gene OrientationClockwise
Centisome Percentage:61.04
Left Sequence End2831934
Right Sequence End2833067
Gene Sequence:
>1134 bp
ATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCG
TCGCCCAGTAATAATGAAATTGATTTACTTAATCTAATCTCAGTTTTATGGCGGGCCAAA
AAAACGGTCATGGCGGTCGTTTTTGCGTTTGCCTGCGCAGGCTTGCTGATCTCTTTCATC
CTGCCGCAAAAATGGACCAGCGCGGCGGTTGTCACGCCTCCAGAACCTGTTCAGTGGCAA
GAGTTGGAGAAATCATTCACTAAGCTTCGTGTGCTGGATCTGGATATCAAAATTGATCGT
ACAGAAGCATTTAACCTGTTTATCAAGAAGTTTCAGTCGGTTAGCTTGCTGGAAGAGTAC
CTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGAT
TTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGT
AAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACC
AGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTG
AAAGAGTCGCTAGAAAACGTCCGTAATAAACTGGAGATCAAAACCCAGTTTGAAAAAGAA
AAACTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTC
AATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGT
CAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGC
AAACTGGAAATAGAAAAAGCGGTCACTGACGTTGCGGAACTGAACGGTGAATTACGTAAT
CGGCAGTATCTTGTCGAGCAATTAACAAAAGCACATGTCAACGATGTGAATTTTACGCCG
TTTAAATATCAGTTAAGCCCGTCATTGCCAGTGAAAAAAGACGGTCCGGGTAAGGCGATT
ATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGC
TATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:377
Protein Molecular Weight:41403
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nitric oxide reductase FlRd-NAD(+) reductase
MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
RQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQATLDRQRNIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
SEDRMKEAFGLLKTLPM
References
External Links:
ResourceLink
Uniprot ID:P37596
Uniprot Name:NORW_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674708
Ecogene ID:EG12450
Ecocyc:EG12450
ColiBase:b2711
Kegg Gene:b2711
EchoBASE ID:EB2344
CCDB:NORW_ECOLI
BacMap:16130618
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Gardner, A. M., Gessner, C. R., Gardner, P. R. (2003). "Regulation of the nitric oxide reduction operon (norRVW) in Escherichia coli. Role of NorR and sigma54 in the nitric oxide stress response." J Biol Chem 278:10081-10086. Pubmed: 12529359
  • Gardner, A. M., Helmick, R. A., Gardner, P. R. (2002). "Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli." J Biol Chem 277:8172-8177. Pubmed: 11751865
  • Gomes, C. M., Giuffre, A., Forte, E., Vicente, J. B., Saraiva, L. M., Brunori, M., Teixeira, M. (2002). "A novel type of nitric-oxide reductase. Escherichia coli flavorubredoxin." J Biol Chem 277:25273-25276. Pubmed: 12101220
  • Gomes, C. M., Vicente, J. B., Wasserfallen, A., Teixeira, M. (2000). "Spectroscopic studies and characterization of a novel electron-transfer chain from Escherichia coli involving a flavorubredoxin and its flavoprotein reductase partner." Biochemistry 39:16230-16237. Pubmed: 11123953
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553