2.02012-05-31 13:58:47 -06002015-09-13 12:56:13 -0600ECMDB03378M2MDB000495Nitric oxideNitric oxide or nitrogen monoxide is a chemical compound with chemical formula NO. This gas is an important signaling molecule in biological systems. It is also cytotoxic. Mononitrogen monoxideNitric oxideNitrogen monoxideNitrogen oxideNitrogen protoxideNitrosyl hydrideNitrosyl radicalNitroxide radicalNitroxylNONO30.006129.997988627nitrosonitric oxide10102-43-9[N]=OInChI=1S/NO/c1-2MWUXSHHQAYIFBG-UHFFFAOYSA-NGasCytosolExtra-organismPeriplasmmelting_point-163.6 oClogp-0.35pka_strongest_basic-2.9iupacnitrosoaverage_mass30.0061mono_mass29.997988627smiles[N]=OformulaNOinchiInChI=1S/NO/c1-2inchikeyMWUXSHHQAYIFBG-UHFFFAOYSA-Npolar_surface_area34.14refractivity2.89polarizability1.69rotatable_bond_count0acceptor_count2donor_count0physiological_charge0formal_charge0Arginine and proline metabolismec00330Nitrogen metabolism
The biological process of the nitrogen cycle is a complex interplay among many microorganisms catalyzing different reactions, where nitrogen is found in various oxidation states ranging from +5 in nitrate to -3 in ammonia.
The ability of fixing atmospheric nitrogen by the nitrogenase enzyme complex is present in restricted prokaryotes (diazotrophs). The other reduction pathways are assimilatory nitrate reduction and dissimilatory nitrate reduction both for conversion to ammonia, and denitrification. Denitrification is a respiration in which nitrate or nitrite is reduced as a terminal electron acceptor under low oxygen or anoxic conditions, producing gaseous nitrogen compounds (N2, NO and N2O) to the atmosphere.
Nitrate can be introduced into the cytoplasm through a nitrate:nitrite antiporter NarK or a nitrate / nitrite transporter NarU. Nitrate is then reduced by a Nitrate Reductase resulting in the release of water, an acceptor and a Nitrite. Nitrite can also be introduced into the cytoplasm through a nitrate:nitrite antiporter NarK
Nitrite can be reduced a NADPH dependent nitrite reductase resulting in water and NAD and Ammonia.
Nitrite can interact with hydrogen ion, ferrocytochrome c through a cytochrome c-552 ferricytochrome resulting in the release of ferricytochrome c, water and ammonia
Another process by which ammonia is produced is by a reversible reaction of hydroxylamine with a reduced acceptor through a hydroxylamine reductase resulting in an acceptor, water and ammonia.
Water and carbon dioxide react through a carbonate dehydratase resulting in carbamic acid. This compound reacts spontaneously with hydrogen ion resulting in the release of carbon dioxide and ammonia. Carbon dioxide can interact with water through a carbonic anhydrase resulting in hydrogen carbonate. This compound interacts with cyanate and hydrogen ion through a cyanate hydratase resulting in a carbamic acid.
Ammonia can be metabolized by reacting with L-glutamine and ATP driven glutamine synthetase resulting in ADP, phosphate and L-glutamine. The latter compound reacts with oxoglutaric acid and hydrogen ion through a NADPH dependent glutamate synthase resulting in the release of NADP and L-glutamic acid. L-glutamic acid reacts with water through a NADP-specific glutamate dehydrogenase resulting in the release of oxoglutaric acid, NADPH, hydrogen ion and ammonia.
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Journal of Physical Chemistry (1985), 89(13), 2914-16.http://hmdb.ca/system/metabolites/msds/000/002/959/original/HMDB03378.pdf?1358463213FlavohemoproteinP24232HMP_ECOLIhmphttp://ecmdb.ca/proteins/P24232.xmlNitric oxide reductase FlRd-NAD(+) reductaseP37596NORW_ECOLInorWhttp://ecmdb.ca/proteins/P37596.xmlAnaerobic nitric oxide reductase flavorubredoxinQ46877NORV_ECOLInorVhttp://ecmdb.ca/proteins/Q46877.xmlOuter membrane protein NP77747OMPN_ECOLIompNhttp://ecmdb.ca/proteins/P77747.xmlOuter membrane pore protein EP02932PHOE_ECOLIphoEhttp://ecmdb.ca/proteins/P02932.xmlOuter membrane protein FP02931OMPF_ECOLIompFhttp://ecmdb.ca/proteins/P02931.xmlOuter membrane protein CP06996OMPC_ECOLIompChttp://ecmdb.ca/proteins/P06996.xmlHydrogen ion + NADH + 2 Nitric oxide > Water + Nitrous oxide + NADNADH + 2 Nitric oxide + 2 Oxygen > Hydrogen ion + NAD +2 NitrateNADPH + 2 Nitric oxide + 2 Oxygen > Hydrogen ion + NADP +2 Nitrate2 [4Fe-4S] iron-sulfur cluster + 2 Hydrogen ion + 2 Nitric oxide >2 [3Fe-4S] damaged iron-sulfur cluster +2 Fe3+ + Water + Nitrous oxideNitrous oxide + Water + Cytochromes-C-Oxidized Nitric oxide + Hydrogen ion + Cytochromes-C-ReducedNITRIC-OXIDE-REDUCTASE-RXNNAD(P)H + Nitric oxide + Oxygen > NAD(P)<sup>+</sup> + Nitrate + Hydrogen ionR621-RXN2 Nitric oxide + 2 Oxygen + NAD(P)H >2 Nitrate + NAD(P)(+)Nitric oxide + 2 Oxygen + NADH + NADPH <>2 Nitrate + NAD + NADP + Hydrogen ionR05724 R05725