ECMDB

Identification

Name:

NADH pyrophosphatase

Synonyms:

Not Available

Gene Name:

nudC

Enzyme Class:

3.6.1.22

Biological Properties

General Function:

Involved in hydrolase activity

Specific Function:

NAD(+) + H(2)O = AMP + NMN

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Nicotinate and nicotinamide metabolism ec00760

KEGG Reactions:

1.0

↔

1.0

2.0

1.0

1.0Water + 1.0NAD ↔ 1.0Adenosine monophosphate + 2.0Hydrogen ion + 1.0Nicotinamide ribotide + 1.0NMN
ReactionCard

1.0

↔

1.0

1.0NAD + 1.0Water ↔ 1.0Adenosine monophosphate + 1.0Nicotinamide ribotide
ReactionCard

1.0

↔

1.0

1.0Nicotinic acid adenine dinucleotide + 1.0Water ↔ 1.0Adenosine monophosphate + 1.0Nicotinamide ribotide
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

2.0

1.0

1.0Water + 1.0NAD ↔ 1.0Adenosine monophosphate + 2.0Hydrogen ion + 1.0Nicotinamide ribotide + 1.0NMN
ReactionCard

1.0

↔

1.0

1.0NAD + 1.0Water ↔ 1.0Adenosine monophosphate + 1.0Nicotinamide ribotide
ReactionCard

1.0

→

1.0

1.0Water + 1.0NAD → 1.0Hydrogen ion + 1.0Nicotinamide ribotide + 1.0Adenosine monophosphate
ReactionCard

1.0

→

1.0

1.0NADH + 1.0Water → 1.0Hydrogen ion + 1.0NMNH + 1.0Adenosine monophosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0NAD + 1.0Water → 1.0Adenosine monophosphate + 1.0NMN
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB00229	Nicotinamide ribotide	MetaboCard
ECMDB01179	Nicotinic acid adenine dinucleotide	MetaboCard
ECMDB23222	NMN	MetaboCard
ECMDB20594	NMNH	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
catalytic activity
cation binding
hydrolase activity
ion binding
metal ion binding

Gene Properties

Blattner:

b3996

Gene Orientation

Clockwise

Centisome Percentage:

90.41

Left Sequence End

4194926

Right Sequence End

4195699

Gene Sequence:

>774 bp
ATGGCTCGTAAATGGTTGAACTTGTTTGCCGGGGCGGCACTCTCTTTCGCTGTTGCTGGC
AATGCACTGGCAGATGAAGGGAAAATCACGGTGTTCGCCGCCGCATCACTGACTAACGCA
ATGCAGGACATTGCTACGCAGTTTAAAAAAGAGAAAGGCGTGGATGTGGTTTCTTCTTTC
GCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATT
TCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAGCGATCGATACAGCTACG
CGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAA
GATTTCACCATCGACAGCAAAACCAACTGGACTTCACTGCTGAATGGCGGTCGCCTGGCG
GTTGGCGATCCGGAACATGTTCCCGCTGGCATTTATGCAAAAGAAGCACTGCAAAAACTG
GGCGCATGGGATACGCTCTCTCCGAAACTGGCCCCAGCGGAAGATGTTCGTGGGGCGCTG
GCGCTGGTCGAACGTAACGAAGCGCCTCTGGGCATTGTCTACGGTTCTGACGCAGTTGCC
AGCAAAGGGGTAAAAGTGGTTGCCACCTTCCCGGAAGATTCACATAAAAAAGTGGAATAT
CCGGTTGCTGTTGTGGAAGGGCATAACAATGCGACAGTGAAAGCATTTTATGATTATCTG
AAGGGACCGCAGGCAGCGGAAATCTTTAAACGTTACGGATTTACAATCAAGTAA

Protein Properties

Pfam Domain Function:

NUDIX (PF00293 )
NUDIX-like (PF09296 )
zf-NADH-PPase (PF09297 )

Protein Residues:

257

Protein Molecular Weight:

29689

Protein Theoretical pI:

PDB File:

1VK6

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>NADH pyrophosphatase
MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRALQIGEWQGEPVWLVQQQ
RRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCGHEMYPSKTEWAMLCSHCR
ERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGFVEVGETLEQAVAREVMEESG
IKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPKELLEANWYRYDDLPLLPPPGTV
ARRLIEDTVAMCRAEYE

References

External Links:

Resource	Link
Uniprot ID:	P32664
Uniprot Name:	NUDC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651348
PDB ID:	1VK6
Ecogene ID:	EG11702
Ecocyc:	EG11702
ColiBase:	b3996
Kegg Gene:	b3996
EchoBASE ID:	EB1653
CCDB:	NUDC_ECOLI
BacMap:	49176450

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Frick, D. N., Bessman, M. J. (1995). "Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes." J Biol Chem 270:1529-1534. Pubmed: 7829480
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293

NADH pyrophosphatase (P32664)