Identification |
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Name: | 2-methylcitrate synthase |
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Synonyms: | - Citrate synthase 2
- Methylcitrate synthase
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Gene Name: | prpC |
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Enzyme Class: | |
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Biological Properties |
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General Function: | Involved in transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer |
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Specific Function: | Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity |
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Cellular Location: | Not Available |
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SMPDB Pathways: | |
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KEGG Pathways: | |
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KEGG Reactions: | |
1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
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SMPDB Reactions: | |
1.0Propionyl-CoA | + | 1.0 | + | 1.0 | + | 1.0 | → | 1.0 | + | 1.0 | + | 1.02-Methylcitric acid | + | 1.0 |
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EcoCyc Reactions: | |
1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 | + | 1.0 | + | 1.0 |
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Metabolites: | |
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GO Classification: | Component |
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cell part | cytoplasm | intracellular part | Function |
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catalytic activity | transferase activity | transferase activity, transferring acyl groups | transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer | Process |
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acetyl-CoA catabolic process | acetyl-CoA metabolic process | carbohydrate metabolic process | cellular carbohydrate metabolic process | cellular metabolic process | coenzyme metabolic process | cofactor metabolic process | metabolic process | primary metabolic process | tricarboxylic acid cycle |
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Gene Properties |
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Blattner: | b0333 |
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Gene Orientation | Clockwise |
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Centisome Percentage: | 7.53 |
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Left Sequence End | 349236 |
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Right Sequence End | 350405 |
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Gene Sequence: | >1170 bp
ATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTG
GCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAAT
GACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAA
GTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAA
ACGAAACTGAAAGCCCTGCGCGGTTTACCGGCTAACGTGCGTACCGTGCTGGAAGCCTTA
CCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACG
CTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTG
GCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATC
CAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAA
AAGCCATCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACAC
GAGTTTAACGCCTCCACCTTTACCAGTCGGGTGATTGCGGGCACCGGCTCTGATATGTAT
TCCGCGATTATTGGCGCGATTGGCGCACTGCGCGGGCCAAAACACGGCGGGGCGAATGAA
GTGTCGCTGGAGATCCAGCAACGCTACGAAACGCCGGACGAAGCCGAAGCAGATATCCGC
AAGCGCGTGGAAAACAAAGAAGTGGTCATTGGTTTTGGTCATCCGGTTTACACCATCGCT
GACCCGCGCCACCAGGTGATTAAACGTGTGGCGAAGCAGCTCTCGCAGGAAGGCGGCTCG
CTGAAGATGTACAACATCGCCGATCGCCTGGAAACGGTGATGTGGGAGAGCAAAAAGATG
TTCCCCAATCTCGACTGGTTCTCTGCTGTTTCCTACAACATGATGGGCGTTCCCACCGAG
ATGTTCACACCACTGTTTGTTATCGCCCGCGTCACCGGCTGGGCGGCGCACATTATCGAA
CAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATTATGTTGGACCGGAAGACCGC
CCGTTTGTCGCGCTGGATAAGCGCCAGTAA |
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Protein Properties |
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Pfam Domain Function: | |
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Protein Residues: | 389 |
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Protein Molecular Weight: | 43102 |
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Protein Theoretical pI: | 7 |
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Signaling Regions: | |
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Transmembrane Regions: | |
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Protein Sequence: | >2-methylcitrate synthase
MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAKHCEFEE
VAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCT
LPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGE
KPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANE
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGS
LKMYNIADRLETVMWESKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIE
QRQDNKIIRPSANYVGPEDRPFVALDKRQ |
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References |
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External Links: | |
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General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Patton, A. J., Hough, D. W., Towner, P., Danson, M. J. (1993). "Does Escherichia coli possess a second citrate synthase gene?" Eur J Biochem 214:75-81. Pubmed: 8508809
- Textor, S., Wendisch, V. F., De Graaf, A. A., Muller, U., Linder, M. I., Linder, D., Buckel, W. (1997). "Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria." Arch Microbiol 168:428-436. Pubmed: 9325432
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