Methionine import ATP-binding protein MetN (P30750)

Identification
Name:Methionine import ATP-binding protein MetN
Synonyms:Not Available
Gene Name:metN
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:Not Available
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Methionine + 1.0Adenosine triphosphate + 1.0Water → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0L-Methionine + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Pyrophosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Methionine + 1.0Adenosine triphosphate + 1.0Water → 1.0Adenosine diphosphate + 1.0Pyrophosphate + 1.0Hydrogen ion + 1.0L-Methionine + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Methionine → 1.0ADP + 1.0Hydrogen ion + 1.0L-Methionine + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0D-Methionine → 1.0ADP + 1.0Hydrogen ion + 1.0D-Methionine + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21203D-MethionineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cell wall
external encapsulating structure
Gram-negative-bacterium-type cell wall
peptidoglycan-based cell wall
Function
active transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
amine transmembrane transporter activity
amino acid transmembrane transporter activity
amino acid-transporting ATPase activity
ATP binding
ATPase activity
binding
catalytic activity
D-methionine transmembrane transporter activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
methionine transmembrane transporter activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
sulfur amino acid transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
D-methionine transport
establishment of localization
methionine transport
sulfur amino acid transport
transport
Gene Properties
Blattner:b0199
Gene OrientationCounterclockwise
Centisome Percentage:4.78
Left Sequence End221614
Right Sequence End222645
Gene Sequence:
>1032 bp
ATGATAAAACTTTCGAATATCACCAAAGTGTTCCACCAGGGCACCCGCACCATCCAGGCG
TTGAACAACGTCAGCCTGCATGTGCCAGCTGGACAAATTTATGGCGTTATCGGTGCCTCA
GGCGCGGGTAAGAGTACGCTTATACGTTGTGTAAACCTGCTGGAGCGCCCAACCGAGGGT
AGCGTGCTGGTCGATGGCCAGGAACTGACCACGCTGTCAGAATCCGAGTTGACCAAAGCT
CGCCGCCAGATTGGTATGATTTTCCAGCATTTTAACCTGCTCTCTTCGCGTACTGTTTTT
GGCAACGTGGCTCTGCCGCTGGAGCTGGACAACACACCGAAAGACGAGGTCAAACGTCGC
GTGACGGAATTGCTGTCATTGGTTGGTCTTGGCGATAAGCATGATAGCTACCCGTCGAAT
CTTTCCGGTGGGCAGAAACAACGTGTGGCAATTGCCCGTGCGTTAGCCAGCAATCCCAAA
GTATTGCTGTGTGATGAAGCCACCAGCGCGCTGGACCCGGCAACGACACGTTCTATTCTC
GAACTGCTGAAAGACATCAACCGCCGTCTGGGGTTGACGATTCTGTTGATCACCCACGAA
ATGGACGTTGTGAAGCGCATTTGTGATTGCGTGGCGGTCATCAGCAATGGAGAACTGATC
GAGCAGGACACGGTAAGTGAAGTGTTCTCGCATCCGAAAACGCCGCTGGCGCAGAAGTTT
ATTCAGTCGACCCTGCATCTGGATATCCCGGAAGATTACCAGGAACGTCTGCAAGCGGAG
CCATTTACTGACTGCGTGCCGATGCTGCGTCTGGAGTTTACCGGTCAATCGGTCGATGCC
CCACTGCTTTCTGAAACCGCGCGTCGTTTCAACGTCAACAACAACATTATTAGCGCGCAG
ATGGATTACGCCGGTGGCGTTAAGTTCGGCATCATGCTGACTGAAATGCACGGCACACAA
CAAGATACGCAAGCCGCCATTGCCTGGCTGCAGGAACACCATGTAAAAGTAGAGGTACTG
GGTTATGTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:343
Protein Molecular Weight:37788
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Methionine import ATP-binding protein MetN
MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGKSTLIRCVNLLERPTEG
SVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGNVALPLELDNTPKDEVKRR
VTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPKVLLCDEATSALDPATTRSIL
ELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGELIEQDTVSEVFSHPKTPLAQKF
IQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFNVNNNIISAQ
MDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV
References
External Links:
ResourceLink
Uniprot ID:P30750
Uniprot Name:METN_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674381
Ecogene ID:EG11621
Ecocyc:EG11621
ColiBase:b0199
Kegg Gene:b0199
EchoBASE ID:EB1578
CCDB:METN_ECOLI
BacMap:16128192
General Reference:
  • Allikmets, R., Gerrard, B., Court, D., Dean, M. (1993). "Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance." Gene 136:231-236. Pubmed: 7904973
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gal, J., Szvetnik, A., Schnell, R., Kalman, M. (2002). "The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster." J Bacteriol 184:4930-4932. Pubmed: 12169620
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Zhang, Z., Feige, J. N., Chang, A. B., Anderson, I. J., Brodianski, V. M., Vitreschak, A. G., Gelfand, M. S., Saier, M. H. Jr (2003). "A transporter of Escherichia coli specific for L- and D-methionine is the prototype for a new family within the ABC superfamily." Arch Microbiol 180:88-100. Pubmed: 12819857