Identification
Name:Protein cyaY
Synonyms:Not Available
Gene Name:cyaY
Enzyme Class:Not Available
Biological Properties
General Function:Inorganic ion transport and metabolism
Specific Function:Specific function unknown
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb+2.0Thumb+1.0Thumb+1.0SufBCD scaffold complex+2.0SufSE with bound sulfur1.0Thumb+1.0Thumb+7.0Thumb+1.0Thumb+1.0SufBCD with bound [2Fe-2S] cluster+2.0SufSE sulfur acceptor complex
1.0Adenosine triphosphate + 1.0FADH2 + 2.0Iron + 1.0Water + 1.0SufBCD scaffold complex + 2.0SufSE with bound sulfur → 1.0ADP + 1.0FAD + 7.0Hydrogen ion + 1.0Phosphate + 1.0SufBCD with bound [2Fe-2S] cluster + 2.0SufSE sulfur acceptor complex
ReactionCard
1.0Thumb+1.0Thumb+2.0Thumb+1.0Thumb+1.0SufBCD with bound [2Fe-2S] cluster+2.0SufSE with bound sulfur1.0Thumb+1.0Thumb+7.0Thumb+1.0Thumb+1.0SufBCD with two bound [2Fe-2S] clusters+2.0SufSE sulfur acceptor complex
1.0Adenosine triphosphate + 1.0FADH2 + 2.0Iron + 1.0Water + 1.0SufBCD with bound [2Fe-2S] cluster + 2.0SufSE with bound sulfur → 1.0ADP + 1.0FAD + 7.0Hydrogen ion + 1.0Phosphate + 1.0SufBCD with two bound [2Fe-2S] clusters + 2.0SufSE sulfur acceptor complex
ReactionCard
1.0Thumb+2.0Thumb+2.0IscS with bound sulfur+1.0IscU scaffold protein1.0Thumb+6.0Thumb+2.0IscS sulfur acceptor protein+1.0IscU with bound [2Fe-2S] cluster
1.0FADH2 + 2.0Iron + 2.0IscS with bound sulfur + 1.0IscU scaffold protein → 1.0FAD + 6.0Hydrogen ion + 2.0IscS sulfur acceptor protein + 1.0IscU with bound [2Fe-2S] cluster
ReactionCard
1.0Thumb+2.0Thumb+2.0IscS with bound sulfur+1.0IscU with bound [2Fe-2S] cluster1.0Thumb+6.0Thumb+2.0IscS sulfur acceptor protein+1.0IscU with two bound [2Fe-2S] clusters
1.0FADH2 + 2.0Iron + 2.0IscS with bound sulfur + 1.0IscU with bound [2Fe-2S] cluster → 1.0FAD + 6.0Hydrogen ion + 2.0IscS sulfur acceptor protein + 1.0IscU with two bound [2Fe-2S] clusters
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01248FADMetaboCard
ECMDB01197FADH2MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00692IronMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3807
Gene OrientationCounterclockwise
Centisome Percentage:86.04
Left Sequence End3991762
Right Sequence End3992082
Gene Sequence:
>321 bp
ATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAACCACCGTCGGC
GCTGCCGAGAGAACTTTAACCCCACAACAACAGCGTATGACCTCCTGTAATCAGCAGGCG
ACGGCGCAGGCGTTGAAAGGGGATGCTCGTAAGACCTACATGAGTGATTGCCTGAAGAAC
AGCAAGTCTGCGCCTGGCGAAAAAAGTTTGACGCCACAGCAGCAAAAGATGCGCGAATGC
AATAATCAAGCAACACAACAATCTCTGAAAGGTGATGATCGTAATAAGTTTATGAGTGCC
TGCCTCAAGAAAGCCGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:106
Protein Molecular Weight:12231
Protein Theoretical pI:4
PDB File:1EW4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Protein cyaY
MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFENGSKIIINRQEPLHQV
WLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGETVSFR
References
External Links:
ResourceLink
Uniprot ID:P27838
Uniprot Name:CYAY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674525
PDB ID:1EW4
Ecogene ID:EG11653
Ecocyc:EG11653
ColiBase:b3807
Kegg Gene:b3807
EchoBASE ID:EB1606
CCDB:CYAY_ECOLI
BacMap:16131659
General Reference:
  • Aiba, H., Mori, K., Tanaka, M., Ooi, T., Roy, A., Danchin, A. (1984). "The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli." Nucleic Acids Res 12:9427-9440. Pubmed: 6393056
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cho, S. J., Lee, M. G., Yang, J. K., Lee, J. Y., Song, H. K., Suh, S. W. (2000). "Crystal structure of Escherichia coli CyaY protein reveals a previously unidentified fold for the evolutionarily conserved frataxin family." Proc Natl Acad Sci U S A 97:8932-8937. Pubmed: 10908679
  • Danchin, A. (1993). "Phylogeny of adenylyl cyclases." Adv Second Messenger Phosphoprotein Res 27:109-162. Pubmed: 8418825
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Li, D. S., Ohshima, K., Jiralerspong, S., Bojanowski, M. W., Pandolfo, M. (1999). "Knock-out of the cyaY gene in Escherichia coli does not affect cellular iron content and sensitivity to oxidants." FEBS Lett 456:13-16. Pubmed: 10452520
  • Trotot, P., Sismeiro, O., Vivares, C., Glaser, P., Bresson-Roy, A., Danchin, A. (1996). "Comparative analysis of the cya locus in enterobacteria and related gram-negative facultative anaerobes." Biochimie 78:277-287. Pubmed: 8874804