| Identification |
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| Name: | Transcriptional regulator nadR |
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| Synonyms: | Not Available |
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| Gene Name: | nadR |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in negative regulation of transcription, DNA-dependent |
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| Specific Function: | NadR is bifunctional; it interacts with pnuC at low internal NAD levels, permitting transport of NMN intact into the cell. As NAD levels increase within the cell, the affinity of nadR for the operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes |
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| Cellular Location: | Membrane; Peripheral membrane protein. Cytoplasm. |
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| SMPDB Pathways: | |
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| KEGG Pathways: | |
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| KEGG Reactions: | |
1.0 | + | 1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 |
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| SMPDB Reactions: | |
1.0 | + | 1.0 | → | 1.0Adenosine diphosphate | + | 1.0 | + | 1.0beta-nicotinamide D-ribonucleotide | + | 1.0 | + | 1.0 |
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1.0beta-nicotinamide D-ribonucleotide | + | 1.0 | + | 1.0 | + | 1.0 | → | 1.0Pyrophosphate | + | 1.0 |
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| EcoCyc Reactions: | |
1.0 | + | 1.0 | + | 1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0 |
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| Complex Reactions: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| binding | | catalytic activity | | DNA binding | | nucleic acid binding | | sequence-specific DNA binding | | transcription regulator activity | | Process |
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| biological regulation | | biosynthetic process | | cellular metabolic process | | coenzyme biosynthetic process | | coenzyme metabolic process | | cofactor metabolic process | | establishment of localization | | metabolic process | | negative regulation of gene expression | | negative regulation of transcription | | pyridine nucleotide biosynthetic process | | regulation of biological process | | regulation of gene expression | | regulation of macromolecule metabolic process | | regulation of metabolic process | | regulation of transcription | | transport |
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| Gene Properties |
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| Blattner: | b4390 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 99.69 |
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| Left Sequence End | 4625338 |
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| Right Sequence End | 4626570 |
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| Gene Sequence: | >1233 bp
ATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTC
TGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATG
TTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCG
TATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGT
CGCCGTCCGGTGATGCTGGCGGGAGTGGTGTGGTTTATCGTCACCTGTCTGGCAATATTG
CTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGT
TTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATC
AAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTG
GGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTG
GCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGC
GAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGC
TTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCC
CAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTG
CAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCG
CGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTATGATTGGTCTATTG
GTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTA
AGTATTTATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTT
GCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATC
TTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTT
AATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAA
GATAAACAGATGGGAAATTCTCACGAAGGGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 410 |
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| Protein Molecular Weight: | 47346 |
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| Protein Theoretical pI: | 5 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Transcriptional regulator nadR
MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP
RQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTV
PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGIQPDLIYTSEE
ADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVK
YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVADGLRSLG
SSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVELVREMMGEQR |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Neuwald, A. F., Berg, D. E., Stauffer, G. V. (1992). "Mutational analysis of the Escherichia coli serB promoter region reveals transcriptional linkage to a downstream gene." Gene 120:1-9. Pubmed: 1327967
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