Cytosine deaminase (P25524)

Identification
Name:Cytosine deaminase
Synonyms:
  • Cytosine aminohydrolase
Gene Name:codA
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Specific Function:Cytosine + H(2)O = uracil + NH(3)
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cytosine + 1.0Water ↔ 1.0Uracil + 1.0Ammonia
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Methylcytosine + 1.0Water ↔ 1.0Thymine + 1.0Ammonia
ReactionCard
1.0Creatinine+1.0Thumb ↔ 1.0N-Methylhydantoin+1.0Thumb
1.0Creatinine + 1.0Water ↔ 1.0N-Methylhydantoin + 1.0Ammonia
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cytosine + 1.0Water ↔ 1.0Uracil + 1.0Ammonia
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Cytosine + 1.0Hydrogen ion + 1.0Water → 1.0Ammonium + 1.0Uracil
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB028945-MethylcytosineMetaboCard
ECMDB00051AmmoniaMetaboCard
ECMDB21186AmmoniumMetaboCard
ECMDB00630CytosineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00262ThymineMetaboCard
ECMDB00300UracilMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Gene Properties
Blattner:b0337
Gene OrientationClockwise
Centisome Percentage:7.66
Left Sequence End355395
Right Sequence End356678
Gene Sequence:
>1284 bp
GTGTCGAATAACGCTTTACAAACAATTATTAACGCCCGGTTACCAGGCGAAGAGGGGCTG
TGGCAGATTCATCTGCAGGACGGAAAAATCAGCGCCATTGATGCGCAATCCGGCGTGATG
CCCATAACTGAAAACAGCCTGGATGCCGAACAAGGTTTAGTTATACCGCCGTTTGTGGAG
CCACATATTCACCTGGACACCACGCAAACCGCCGGACAACCGAACTGGAATCAGTCCGGC
ACGCTGTTTGAAGGCATTGAACGCTGGGCCGAGCGCAAAGCGTTATTAACCCATGACGAT
GTGAAACAACGCGCATGGCAAACGCTGAAATGGCAGATTGCCAACGGCATTCAGCATGTG
CGTACCCATGTCGATGTTTCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTG
AAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATT
TTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTA
GTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAA
ACCTTCGCCCTGGCGCAAAAATACGACCGTCTCATCGACGTTCACTGTGATGAGATCGAT
GACGAGCAGTCGCGCTTTGTCGAAACCGTTGCTGCCCTGGCGCACCATGAAGGCATGGGC
GCGCGAGTCACCGCCAGCCACACCACGGCAATGCACTCCTATAACGGGGCGTATACCTCA
CGCCTGTTCCGCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCAACCCGCTGGTCAAT
ATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCGTTAAA
GAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCCGTGG
TATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAG
TTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGG
ACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTG
CCGGCTGAAAATGGGTTTGATGCGCTGCGCCGTCAGGTTCCGGTACGTTATTCGGTACGT
GGCGGCAAGGTGATTGCCAGCACACAACCGGCACAAACCACCGTATATCTGGAGCAGCCA
GAAGCCATCGATTACAAACGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:427
Protein Molecular Weight:47591
Protein Theoretical pI:6
PDB File:1K70
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cytosine deaminase
MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVE
PHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHV
RTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADV
VGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMG
ARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVK
EMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSAR
TLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQP
EAIDYKR
References
External Links:
ResourceLink
Uniprot ID:P25524
Uniprot Name:CODA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674479
PDB ID:1K70
Ecogene ID:EG11326
Ecocyc:EG11326
ColiBase:b0337
Kegg Gene:b0337
EchoBASE ID:EB1302
CCDB:CODA_ECOLI
BacMap:16128322
General Reference:
  • Austin, E. A., Huber, B. E. (1993). "A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase." Mol Pharmacol 43:380-387. Pubmed: 8450832
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Danielsen, S., Kilstrup, M., Barilla, K., Jochimsen, B., Neuhard, J. (1992). "Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase." Mol Microbiol 6:1335-1344. Pubmed: 1640834
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ireton, G. C., McDermott, G., Black, M. E., Stoddard, B. L. (2002). "The structure of Escherichia coli cytosine deaminase." J Mol Biol 315:687-697. Pubmed: 11812140