S-(hydroxymethyl)glutathione dehydrogenase (P25437)

Identification
Name:S-(hydroxymethyl)glutathione dehydrogenase
Synonyms:
  • Alcohol dehydrogenase class-3
  • Alcohol dehydrogenase class-III
  • Glutathione-dependent formaldehyde dehydrogenase
  • FALDH
  • FDH
  • GSH-FDH
Gene Name:frmA
Enzyme Class:
Biological Properties
General Function:Involved in zinc ion binding
Specific Function:Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Collection of Reactions without pathways PW001891
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ethanol + 1.0NAD ↔ 1.0Acetaldehyde + 1.0Hydrogen ion + 1.0NADH
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-(Hydroxymethyl)glutathione + 1.0NAD ↔ 1.0S-Formylglutathione + 1.0Hydrogen ion + 1.0NADH
ReactionCard
1.0Primary alcohol+1.0Thumb ↔ 1.0Aldehyde+1.0Thumb+1.0Thumb
1.0Primary alcohol + 1.0NAD ↔ 1.0Aldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Retinol + 1.0NAD ↔ 1.0Retinal + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0trans-3-Chloro-2-propene-1-ol+1.0Thumb ↔ 1.0trans-3-Chloroallyl aldehyde+1.0Thumb+1.0Thumb
1.0trans-3-Chloro-2-propene-1-ol + 1.0NAD ↔ 1.0trans-3-Chloroallyl aldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0cis-3-Chloro-2-propene-1-ol+1.0Thumb ↔ 1.0cis-3-Chloroallyl aldehyde+1.0Thumb+1.0Thumb
1.0cis-3-Chloro-2-propene-1-ol + 1.0NAD ↔ 1.0cis-3-Chloroallyl aldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0(2-Naphthyl)methanol + 1.0NAD ↔ 1.02-Naphthaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Chloral hydrate+1.0Thumb+1.0Thumb ↔ 1.0Trichloroethanol+1.0Thumb+1.0Thumb
1.0Chloral hydrate + 1.0NADH + 1.0Hydrogen ion ↔ 1.0Trichloroethanol + 1.0NAD + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Phenyl-1,3-propanediol monocarbamate + 1.0NAD ↔ 1.03-Carbamoyl-2-phenylpropionaldehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.04-Hydroxy-5-phenyltetrahydro-1,3-oxazin-2-one + 1.0NAD ↔ 1.05-Phenyl-1,3-oxazinane-2,4-dione + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0S-(Hydroxymethyl)glutathione + 1.0NAD + 1.0NADP ↔ 1.0S-Formylglutathione + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0(2-Naphthyl)methanol + 1.0NAD → 1.02-Naphthaldehyde + 1.0Hydrogen ion + 1.0NADH
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ethanol + 1.0NAD ↔ 1.0Acetaldehyde + 1.0Hydrogen ion + 1.0NADH
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Glyceraldehyde + 1.0Hydrogen ion + 1.0NADH ↔ 1.0Glycerol + 1.0NAD
ReactionCard
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.0S-(Hydroxymethyl)glutathione + 1.0NAD(P)(+) → 1.0S-Formylglutathione + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20007(2-Naphthyl)methanolMetaboCard
ECMDB200211-HydroxymethylnaphthaleneMetaboCard
ECMDB200472-NaphthaldehydeMetaboCard
ECMDB200482-Phenyl-1,3-propanediol monocarbamateMetaboCard
ECMDB003183,4-DihydroxyphenylglycolMetaboCard
ECMDB200653-Carbamoyl-2-phenylpropionaldehydeMetaboCard
ECMDB200914-Hydroxy-5-phenyltetrahydro-1,3-oxazin-2-oneMetaboCard
ECMDB201075-Phenyl-1,3-oxazinane-2,4-dioneMetaboCard
ECMDB00990AcetaldehydeMetaboCard
ECMDB21646AldehydeMetaboCard
ECMDB01051D-GlyceraldehydeMetaboCard
ECMDB00108EthanolMetaboCard
ECMDB00131GlycerolMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB21659RetinalMetaboCard
ECMDB21660RetinolMetaboCard
ECMDB04662S-(Hydroxymethyl)glutathioneMetaboCard
ECMDB01550S-FormylglutathioneMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
metal ion binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
S-(hydroxymethyl)glutathione dehydrogenase activity
transition metal ion binding
zinc ion binding
Process
alcohol metabolic process
ethanol metabolic process
ethanol oxidation
metabolic process
monohydric alcohol metabolic process
oxidation reduction
small molecule metabolic process
Gene Properties
Blattner:b0356
Gene OrientationCounterclockwise
Centisome Percentage:8.14
Left Sequence End377686
Right Sequence End378795
Gene Sequence:
>1110 bp
ATGAAATCACGTGCTGCCGTTGCATTTGCTCCCGGTAAACCGCTGGAAATCGTTGAAATT
GACGTTGCACCACCGAAAAAAGGTGAAGTGCTAATTAAAGTCACCCATACCGGCGTTTGC
CATACCGACGCATTTACCCTCTCCGGCGATGACCCGGAAGGTGTATTCCCGGTGGTTCTC
GGTCACGAAGGGGCCGGCGTTGTGGTTGAAGTCGGTGAAGGCGTAACCAGCGTCAAACCT
GGCGACCATGTGATCCCGCTTTACACCGCGGAGTGCGGCGAGTGTGAGTTCTGTCGTTCT
GGCAAAACTAACCTCTGTGTTGCGGTTCGCGAAACCCAGGGTAAAGGCCTGATGCCAGAC
GGCACCACCCGTTTTTCTTACAACGGGCAGCCGCTTTATCACTACATGGGGTGCTCTACA
TTCAGTGAATACACCGTAGTCGCGGAAGTGTCTCTGGCAAAAATTAATCCAGAAGCAAAC
CATGAACACGTCTGCCTGCTGGGCTGTGGCGTGACCACCGGTATTGGCGCGGTACACAAC
ACAGCTAAAGTCCAGCCAGGTGATTCTGTTGCCGTGTTTGGTCTTGGCGCGATTGGTCTG
GCAGTGGTTCAGGGCGCGCGTCAGGCGAAAGCGGGTCGGATTATCGCTATCGATACCAAC
CCGAAGAAATTCGATCTGGCGCGTCGCTTCGGTGCTACCGACTGCATTAACCCGAATGAC
TACGACAAACCGATAAAAGATGTCCTGTTGGATATCAACAAATGGGGTATCGACCATACC
TTTGAATGCATCGGTAACGTCAACGTGATGCGTGCGGCGCTGGAAAGTGCGCACCGCGGC
TGGGGTCAGTCGGTGATCATCGGGGTCGCGGTTGCCGGTCAGGAAATCTCCACCCGTCCA
TTCCAGTTGGTCACTGGTCGCGTATGGAAAGGTTCCGCGTTTGGCGGCGTGAAAGGTCGT
TCCCAGTTACCGGGCATGGTTGAAGATGCGATGAAAGGTGATATCGATCTGGAACCGTTT
GTCACGCATACCATGAGCCTGGATGAAATTAATGACGCCTTCGACCTGATGCATGAAGGC
AAATCCATTCGAACCGTAATTCGTTACTGA
Protein Properties
Pfam Domain Function:
Protein Residues:369
Protein Molecular Weight:39359
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>S-(hydroxymethyl)glutathione dehydrogenase
MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVL
GHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPD
GTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHN
TAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPND
YDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAVAGQEISTRP
FQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEG
KSIRTVIRY
References
External Links:
ResourceLink
Uniprot ID:P25437
Uniprot Name:FRMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674498
Ecogene ID:EG50010
Ecocyc:EG50010
ColiBase:b0356
Kegg Gene:b0356
EchoBASE ID:EB4303
CCDB:FRMA_ECOLI
BacMap:16128341
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gutheil, W. G., Holmquist, B., Vallee, B. L. (1992). "Purification, characterization, and partial sequence of the glutathione-dependent formaldehyde dehydrogenase from Escherichia coli: a class III alcohol dehydrogenase." Biochemistry 31:475-481. Pubmed: 1731906
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Herring, C. D., Blattner, F. R. (2004). "Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR." J Bacteriol 186:6714-6720. Pubmed: 15466022