ECMDB: High-affinity branched-chain amino acid transport ATP-binding protein livF (P22731)

Identification

Name:

High-affinity branched-chain amino acid transport ATP-binding protein livF

Synonyms:

LIV-I protein F

Gene Name:

livF

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in nucleotide binding

Specific Function:

Component of the leucine-specific transport system

Cellular Location:

Cytoplasmic

SMPDB Pathways:

isoleucine biosynthesis PW000818
tRNA charging PW000799

KEGG Pathways:

ABC transporters ec02010

SMPDB Reactions:

1.0

→

1.0

1.0Adenosine diphosphate

1.0

1.0L-Leucine + 1.0Adenosine triphosphate + 1.0Water → 1.0L-Leucine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard

1.0L-Valine

1.0

→

1.0L-Valine

1.0Adenosine diphosphate

1.0Pyrophosphate

1.0

1.0L-Valine + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Valine → 1.0L-Valine + 1.0Adenosine diphosphate + 1.0Pyrophosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard

1.0L-Isoleucine

1.0

→

1.0L-Isoleucine

1.0Adenosine diphosphate

1.0

1.0L-Isoleucine + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Isoleucine → 1.0L-Isoleucine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0L-Leucine → 1.0ADP + 1.0Hydrogen ion + 1.0L-Leucine + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0L-Isoleucine → 1.0ADP + 1.0Hydrogen ion + 1.0L-Isoleucine + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0L-Valine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0L-Valine
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0L-Alanine → 1.0ADP + 1.0L-Alanine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0L-Threonine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0L-Threonine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00161	L-Alanine	MetaboCard
ECMDB00172	L-Isoleucine	MetaboCard
ECMDB00687	L-Leucine	MetaboCard
ECMDB00167	L-Threonine	MetaboCard
ECMDB00883	L-Valine	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity

Gene Properties

Blattner:

b3454

Gene Orientation

Counterclockwise

Centisome Percentage:

77.39

Left Sequence End

3590747

Right Sequence End

3591460

Gene Sequence:

>714 bp
ATGGAATCATACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTA
AACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGA
GCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATA
GGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGGATATCAACAAGATAAATGCCGCC
ATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTA
GTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGT
ATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATG
ACGTTGACAGGGTTGGCCCTGGTAATCATCGTGAATGCGGCATTAATGAGTGAACGGCCC
ATTTGGATAATAAGTTGCTTAATGATTGTGTTATGGTCAGGCATTATCTCGCATGGACGA
AATAAGCTCCTTGAATTGGCGGGGAAATGCCATAGTGAAGAGTTGTGGAGTCCGGTTCGT
TGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTT
GCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAG

Protein Properties

Pfam Domain Function:

ABC_tran (PF00005 )

Protein Residues:

237

Protein Molecular Weight:

26310

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>High-affinity branched-chain amino acid transport ATP-binding protein livF
MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGKTTLLGTLCGDPRATSG
RIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLAMGGFFAERDQFQERIKWV
YELFPRLHERRIQRAGTMSGGEQQMLAIGRALMSNPRLLLLDEPSLGLAPIIIQQIFDTI
EQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLSDTGDALLANEAVRSAYLGG

References

External Links:

Resource	Link
Uniprot ID:	P22731
Uniprot Name:	LIVF_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062344
Ecogene ID:	EG10536
Ecocyc:	EG10536
ColiBase:	b3454
Kegg Gene:	b3454
EchoBASE ID:	EB0531
CCDB:	LIVF_ECOLI
BacMap:	90111594

General Reference:

Adams, M. D., Wagner, L. M., Graddis, T. J., Landick, R., Antonucci, T. K., Gibson, A. L., Oxender, D. L. (1990). "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." J Biol Chem 265:11436-11443. Pubmed: 2195019
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620