ECMDB: NH(3)-dependent NAD(+) synthetase (P18843)

Identification

Name:

NH(3)-dependent NAD(+) synthetase

Synonyms:

Nicotinamide adenine dinucleotide synthetase
NADS
Nitrogen regulatory protein

Gene Name:

nadE

Enzyme Class:

6.3.1.5

Biological Properties

General Function:

Involved in NAD+ synthase (glutamine-hydrolyzing) activity

Specific Function:

Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction

Cellular Location:

Not Available

SMPDB Pathways:

NAD biosynthesis PW000829
NAD salvage PW000830
Nitrogen metabolism PW000755

KEGG Pathways:

Metabolic pathways eco01100
Nicotinate and nicotinamide metabolism ec00760
Nitrogen metabolism ec00910

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Nicotinic acid adenine dinucleotide + 1.0Ammonia ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0NAD
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Pyrophosphate

1.0L-Glutamic acid

1.0

1.0Nicotinic acid adenine dinucleotide + 1.0Water + 1.0L-Glutamine + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-Glutamic acid + 1.0NAD + 1.0L-Glutamate
ReactionCard

1.0

→

1.0

1.0Pyrophosphate

1.0

1.0Nicotinic acid adenine dinucleotide + 1.0Adenosine triphosphate + 1.0Ammonium → 1.0Hydrogen ion + 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0NAD
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Nicotinic acid adenine dinucleotide + 1.0Ammonia ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0NAD
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Nicotinic acid adenine dinucleotide + 1.0Ammonium → 1.0Adenosine monophosphate + 1.0Hydrogen ion + 1.0NAD + 1.0Pyrophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00641	L-Glutamine	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01179	Nicotinic acid adenine dinucleotide	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
carbon-nitrogen ligase activity, with glutamine as amido-N-donor
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
NAD+ synthase (glutamine-hydrolyzing) activity
nucleoside binding
purine nucleoside binding
Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
NAD biosynthetic process
nicotinamide nucleotide biosynthetic process
pyridine nucleotide biosynthetic process

Gene Properties

Blattner:

b1740

Gene Orientation

Clockwise

Centisome Percentage:

39.24

Left Sequence End

1820482

Right Sequence End

1821309

Gene Sequence:

>828 bp
ATGAGTGTGCTCATTAATGAAAAACTGCATTCGCGGCGGCTGAAATGGCGCTGGCCGCTC
TCGCGTCAGGTGACCTTAAGCATTGGCACGTTAGCGGTTTTACTCACCGTATGGTGGACG
GTGGCGACGCTGCAACTGATTAGCCCGCTATTTTTGCCGCCGCCGCAACAGGTACTGGAA
AAACTACTCACCATTGCCGGACCGCAAGGCTTTATGGACGCCACGCTGTGGCAGCATCTG
GCAGCCAGTCTGACGCGCATTATGCTGGCGCTATTTGCAGCGGTGTTGTTCGGTATTCCG
GTCGGGATCGCGATGGGACTTAGCCCTACGGTACGCGGCATTCTGGATCCGATAATCGAG
CTTTATCGTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATT
GGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCG
GCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGTTCGCATTCGTGCCGCCCAGTCGCTGGGT
GCCAGCCGTGCGCAGGTGCTGTGGTTTGTCATTTTGCCCGGTGCGCTGCCGGAAATCCTC
ACCGGATTACGTATTGGTCTGGGGGTGGGCTGGTCTACGCTGGTGGCGGCGGAGCTGATT
GCCGCGACGCGCGGTTTAGGATTTATGGTTCAGTCAGCGGGTGAATTTCTCGCAACTGAC
GTGGTGCTGGCGGGGATCGCGGTGATTGCGATTATCGCCTTTCTTTTAGAACTGGGTCTG
CGCGCGTTACAGCGCCGCCTGACGCCCTGGCATGGAGAAGTACAATGA

Protein Properties

Pfam Domain Function:

NAD_synthase (PF02540 )

Protein Residues:

275

Protein Molecular Weight:

30637

Protein Theoretical pI:

PDB File:

1WXI

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>NH(3)-dependent NAD(+) synthetase
MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLAGKLC
QMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQ
ALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGTDHAAEAITGFFTKYGDGGTD
INPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDRPSLPDEVALGVTYDNIDDYLEG
KNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK

References

External Links:

Resource	Link
Uniprot ID:	P18843
Uniprot Name:	NADE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674508
PDB ID:	1WXI
Ecogene ID:	EG10663
Ecocyc:	EG10663
ColiBase:	b1740
Kegg Gene:	b1740
EchoBASE ID:	EB0657
CCDB:	NADE_ECOLI
BacMap:	16129694

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Allibert, P., Willison, J. C., Vignais, P. M. (1987). "Complementation of nitrogen-regulatory (ntr-like) mutations in Rhodobacter capsulatus by an Escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product." J Bacteriol 169:260-271. Pubmed: 3025172
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Jauch, R., Humm, A., Huber, R., Wahl, M. C. (2005). "Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements." J Biol Chem 280:15131-15140. Pubmed: 15699042
Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
Willison, J. C. (1992). "An essential gene (efg) located at 38.1 minutes on the Escherichia coli chromosome." J Bacteriol 174:5765-5766. Pubmed: 1512214
Willison, J. C., Tissot, G. (1994). "The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase." J Bacteriol 176:3400-3402. Pubmed: 8195100