Identification
Name:Choline dehydrogenase
Synonyms:
  • CDH
  • CHD
Gene Name:betA
Enzyme Class:
Biological Properties
General Function:Involved in choline dehydrogenase activity
Specific Function:Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine at the same rate
Cellular Location:Cell membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acceptor+1.0Acceptor1.0Thumb+1.0Reduced acceptor+1.0Reduced acceptor
1.0Choline + 1.0Acceptor + 1.0Acceptor ↔ 1.0Betaine aldehyde + 1.0Reduced acceptor + 1.0Reduced acceptor
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0an oxidized electron acceptor1.0Thumb+1.0a reduced electron acceptor
1.0Choline + 1.0an oxidized electron acceptor → 1.0Betaine aldehyde + 1.0a reduced electron acceptor
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0acceptor1.0Thumb+1.0reduced acceptor
1.0Choline + 1.0acceptor → 1.0Betaine aldehyde + 1.0reduced acceptor
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01252Betaine aldehydeMetaboCard
ECMDB00097CholineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
choline dehydrogenase activity
FAD or FADH2 binding
nucleoside binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
purine nucleoside binding
Process
alcohol metabolic process
choline metabolic process
ethanolamine and derivative metabolic process
glycine betaine biosynthetic process from choline
metabolic process
oxidation reduction
small molecule metabolic process
Gene Properties
Blattner:b0311
Gene OrientationCounterclockwise
Centisome Percentage:7.00
Left Sequence End324801
Right Sequence End326471
Gene Sequence:
>1671 bp
TTGCAATTTGACTACATCATTATTGGTGCCGGCTCAGCCGGCAACGTTCTCGCTACCCGT
CTGACTGAAGATCCGAATACCTCCGTGCTGCTGCTTGAAGCGGGCGGCCCGGACTATCGC
TTTGACTTCCGCACCCAGATGCCCGCTGCCCTGGCATTCCCGCTACAGGGTAAACGCTAC
AACTGGGCCTATGAAACGGAACCTGAACCGTTTATGAATAACCGCCGCATGGAGTGCGGA
CGCGGTAAAGGTCTGGGTGGATCGTCGCTGATCAACGGCATGTGCTACATCCGTGGCAAT
GCGCTGGATCTCGATAACTGGGCGCAAGAACCCGGTCTGGAGAACTGGAGCTACCTCGAC
TGCCTGCCCTACTACCGCAAGGCCGAGACTCGCGATATGGGTGAAAACGACTATCACGGC
GGTGATGGCCCGGTGAGCGTCACTACCTCCAAACCCGGCGTCAATCCGCTGTTTGAAGCG
ATGATTGAAGCGGGCGTGCAGGCGGGCTACCCGCGCACGGACGATCTCAACGGTTATCAG
CAGGAAGGTTTTGGTCCGATGGATCGCACCGTCACGCCGCAGGGCCGTCGCGCCAGCACC
GCGCGTGGCTATCTCGATCAGGCCAAATCGCGTCCTAACCTGACCATTCGTACTCACGCT
ATGACCGATCACATCATTTTTGACGGCAAACGCGCGGTGGGCGTCGAATGGCTGGAAGGC
GACAGCACCATCCCAACCCGCGCAACGGCCAACAAAGAAGTGCTGTTATGTGCAGGCGCG
ATTGCCTCACCGCAGATCCTGCAACGCTCCGGCGTCGGCAACGCTGAACTGCTGGCGGAG
TTTGATATTCCGCTGGTGCATGAATTACCCGGCGTCGGCGAAAATCTTCAGGATCATCTG
GAGATGTATCTGCAATATGAGTGCAAAGAACCGGTTTCCCTCTACCCTGCCCTGCAGTGG
TGGAACCAGCCGAAAATCGGTGCGGAGTGGCTGTTTGGCGGCACTGGCGTTGGTGCCAGC
AACCACTTTGAAGCAGGTGGATTTATTCGCAGCCGTGAGGAATTTGCGTGGCCGAATATT
CAGTACCATTTCCTGCCAGTAGCGATTAACTATAACGGCTCGAATGCAGTGAAAGAGCAC
GGTTTCCAGTGCCACGTCGGCTCAATGCGCTCGCCAAGCCGTGGGCATGTGCGGATTAAA
TCCCGCGACCCGCACCAGCATCCGGCGATTCTGTTTAACTACATGTCGCACGAGCAGGAC
TGGCAGGAGTTCCGCGACGCAATTCGCATCACCCGCGAGATCATGCATCAACCCGCGCTG
GATCAGTATCGTGGCCGCGAAATCAGCCCCGGTGTCGAATGCCAGACGGATGAACAGCTC
GATGAGTTCGTGCGTAACCACGCCGAAACCGCCTTCCATCCGTGCGGTACCTGCAAAATG
GGTTACGACGAGATGTCCGTGGTTGACGGCGAAGGCCGCGTACACGGGTTAGAAGGCCTG
CGTGTGGTGGATGCGTCGATTATGCCGCAGATTATCACCGGGAATTTGAACGCCACGACA
ATTATGATTGGCGAGAAAATAGCGGATATGATTCGTGGACAGGAAGCGCTGCCGAGGAGC
ACGGCGGGATATTTTGTGGCAAATGGGATGCCGGTGAGAGCGAAAAAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:556
Protein Molecular Weight:61877
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Choline dehydrogenase
MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFRTQMPAALAFPLQGKRY
NWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNALDLDNWAQEPGLENWSYLD
CLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEAMIEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRTHAMTDHIIFDGKRAVGVEWLEG
DSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAELLAEFDIPLVHELPGVGENLQDHL
EMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFAWPNI
QYHFLPVAINYNGSNAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQD
WQEFRDAIRITREIMHQPALDQYRGREISPGVECQTDEQLDEFVRNHAETAFHPCGTCKM
GYDEMSVVDGEGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADMIRGQEALPRS
TAGYFVANGMPVRAKK
References
External Links:
ResourceLink
Uniprot ID:P17444
Uniprot Name:BETA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674454
Ecogene ID:EG10109
Ecocyc:EG10109
ColiBase:b0311
Kegg Gene:b0311
EchoBASE ID:EB0107
CCDB:BETA_ECOLI
BacMap:16128296
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Boyd, L. A., Adam, L., Pelcher, L. E., McHughen, A., Hirji, R., Selvaraj, G. (1991). "Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): structural similarity to mammalian ALDHs and a plant BADH." Gene 103:45-52. Pubmed: 1879697
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lamark, T., Kaasen, I., Eshoo, M. W., Falkenberg, P., McDougall, J., Strom, A. R. (1991). "DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli." Mol Microbiol 5:1049-1064. Pubmed: 1956285