Putative phosphonates transport system permease protein phnE (P16683)

Identification
Name:Putative phosphonates transport system permease protein phnE
Synonyms:Not Available
Gene Name:phnE
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:Not Available
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0DL-O-Phosphoserine → 1.0Hydrogen ion + 1.0Phosphate + 1.0Adenosine diphosphate + 1.0DL-O-Phosphoserine + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Aminopropylphosphonate + 1.0Adenosine triphosphate + 1.0Water → 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB203293-AminopropylphosphonateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24073DL-O-PhosphoserineMetaboCard
ECMDB20327EthylphosphonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20326MethylphosphonateMetaboCard
ECMDB03484O-PhosphohomoserineMetaboCard
ECMDB20330PhenylphosphonateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB20468PhosphonateMetaboCard
ECMDB20328PropylphosphonateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
integral to membrane
integral to plasma membrane
intrinsic to membrane
membrane
membrane part
Function
anion transmembrane transporter activity
ion transmembrane transporter activity
organic anion transmembrane transporter activity
phosphonate transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
organic anion transport
phosphonate transport
transport
Gene Properties
Blattner:b4104
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>780 bp
ATGAACTCGCATAATATTACTAACGAATCATTAGCACTGGCATTAATGCTGGTGGTGGTG
GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTC
GGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTG
GATGATGCCAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAAC
GCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACG
GTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCG
ATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTG
TGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTG
AGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCG
GCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATG
ATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATG
GTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACC
TATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:259
Protein Molecular Weight:28383
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • 13-33
  • 75-95
  • 123-143
  • 176-196
  • 205-225
  • 230-250
Protein Sequence:
>Putative phosphonates transport system permease protein phnE
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFS
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI
References
External Links:
ResourceLink
Uniprot ID:P16683
Uniprot Name:PHNE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674630
ColiBase:b4104
Kegg Gene:b4104
EchoBASE ID:EB4382
CCDB:PHNE_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Chen, C. M., Ye, Q. Z., Zhu, Z. M., Wanner, B. L., Walsh, C. T. (1990). "Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." J Biol Chem 265:4461-4471. Pubmed: 2155230
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Makino, K., Kim, S. K., Shinagawa, H., Amemura, M., Nakata, A. (1991). "Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." J Bacteriol 173:2665-2672. Pubmed: 1840580