ECMDB: Putative phosphonates transport system permease protein phnE (P16683)

Identification

Name:

Putative phosphonates transport system permease protein phnE

Synonyms:

Not Available

Gene Name:

phnE

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in transporter activity

Specific Function:

Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

inner membrane transport PW000786
serine biosynthesis and metabolism PW000809

KEGG Pathways:

Not Available

Transports:

Transport References:

Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590

SMPDB Reactions:

1.0

→

1.0

1.0Adenosine diphosphate

1.0

1.0Adenosine triphosphate + 1.0Water + 1.0DL-O-Phosphoserine → 1.0Hydrogen ion + 1.0Phosphate + 1.0Adenosine diphosphate + 1.0DL-O-Phosphoserine + 1.0ADP
ReactionCard

1.0

→

1.0

1.03-Aminopropylphosphonate + 1.0Adenosine triphosphate + 1.0Water → 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20329	3-Aminopropylphosphonate	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB24073	DL-O-Phosphoserine	MetaboCard
ECMDB20327	Ethylphosphonate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB20326	Methylphosphonate	MetaboCard
ECMDB03484	O-Phosphohomoserine	MetaboCard
ECMDB20330	Phenylphosphonate	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB20468	Phosphonate	MetaboCard
ECMDB20328	Propylphosphonate	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Component
cell part
integral to membrane
integral to plasma membrane
intrinsic to membrane
membrane
membrane part
Function
anion transmembrane transporter activity
ion transmembrane transporter activity
organic anion transmembrane transporter activity
phosphonate transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
organic anion transport
phosphonate transport
transport

Gene Properties

Blattner:

b4104

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>780 bp
ATGAACTCGCATAATATTACTAACGAATCATTAGCACTGGCATTAATGCTGGTGGTGGTG
GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTC
GGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTG
GATGATGCCAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAAC
GCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACG
GTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCG
ATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTG
TGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTG
AGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCG
GCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATG
ATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATG
GTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACC
TATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGA

Protein Properties

Pfam Domain Function:

BPD_transp_1 (PF00528 )

Protein Residues:

259

Protein Molecular Weight:

28383

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

13-33
75-95
123-143
176-196
205-225
230-250

Protein Sequence:

>Putative phosphonates transport system permease protein phnE
MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFS
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI

References

External Links:

Resource	Link
Uniprot ID:	P16683
Uniprot Name:	PHNE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674630
ColiBase:	b4104
Kegg Gene:	b4104
EchoBASE ID:	EB4382
CCDB:	PHNE_ECOLI

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Chen, C. M., Ye, Q. Z., Zhu, Z. M., Wanner, B. L., Walsh, C. T. (1990). "Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." J Biol Chem 265:4461-4471. Pubmed: 2155230
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Makino, K., Kim, S. K., Shinagawa, H., Amemura, M., Nakata, A. (1991). "Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." J Bacteriol 173:2665-2672. Pubmed: 1840580