ECMDB: Quinoprotein glucose dehydrogenase (P15877)

Identification

Name:

Quinoprotein glucose dehydrogenase

Synonyms:

Glucose dehydrogenase [pyrroloquinoline-quinone]

Gene Name:

gcd

Enzyme Class:

1.1.5.2

Biological Properties

General Function:

Involved in oxidoreductase activity, acting on CH-OH group of donors

Specific Function:

GDH is probably involved in energy conservation rather than in sugar metabolism

Cellular Location:

Cell inner membrane; Multi-pass membrane protein; Periplasmic side

SMPDB Pathways:

Not Available

KEGG Pathways:

Pentose phosphate pathway ec00030

KEGG Reactions:

1.0

↔

1.0

1.0D-Glucose + 1.0Ubiquinone-1 ↔ 1.0Gluconolactone + 1.0Ubiquinol-8
ReactionCard

EcoCyc Reactions:

1.0

1.0a ubiquinone

→

1.0

1.0a ubiquinol

1.0b-D-Glucose + 1.0a ubiquinone → 1.0Gluconolactone + 1.0a ubiquinol
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Ubiquinone-8 + 1.0D-Glucose + 1.0Water → 1.0Ubiquinol-8 + 1.0Gluconic acid + 1.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0D-Glucose + 1.0Ubiquinone-10 → 1.0Gluconolactone + 1.0Ubiquinol-1
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00516	b-D-Glucose	MetaboCard
ECMDB00122	D-Glucose	MetaboCard
ECMDB00625	Gluconic acid	MetaboCard
ECMDB00150	Gluconolactone	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21461	Pyrroloquinoline quinone	MetaboCard
ECMDB21574	Ubiquinol-1	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21438	Ubiquinone-1	MetaboCard
ECMDB01072	Ubiquinone-10	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane part
outer membrane-bounded periplasmic space
periplasmic space
Function
binding
catalytic activity
cofactor binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
quinone binding
Process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b0124

Gene Orientation

Counterclockwise

Centisome Percentage:

2.99

Left Sequence End

138835

Right Sequence End

141225

Gene Sequence:

>2391 bp
ATGGCAATTAACAATACAGGCTCGCGACGATTACTCGTCACGCTAACAGCCCTTTTTGCA
GCGCTTTGCGGGCTGTATCTACTCATTGGCGGAGGCTGGCTGGTCGCGATTGGCGGCTCC
TGGTACTACCCTATCGCTGGCCTTGTGATGCTCGGCGTCGCCTGGATGCTGTGGCGCAGT
AAACGCGCCGCGCTTTGGCTATACGCAGCCCTGCTGCTCGGCACCATGATTTGGGGCGTC
TGGGAAGTTGGTTTCGACTTCTGGGCGCTGACTCCGCGCAGCGACATTCTGGTCTTCTTC
GGCATCTGGCTGATCCTGCCGTTTGTCTGGCGTCGCCTGGTCATTCCTGCCAGCGGCGCA
GTTGCCGCACTGGTGGTCGCACTGCTGATTAGCGGTGGTATCCTGACCTGGGCCGGATTT
AACGATCCGCAGGAGATCAACGGCACCTTAAGCGCCGATGCCACACCTGCTGAAGCTATC
TCCCCCGTAGCCGATCAGGACTGGCCTGCCTATGGTCGTAATCAGGAAGGTCAACGCTTT
TCGCCGCTGAAACAAATTAACGCCGATAACGTCCATAATCTGAAAGAAGCCTGGGTGTTC
CGTACTGGCGATGTGAAGCAGCCGAACGATCCGGGTGAAATCACCAATGAAGTGACGCCG
ATTAAAGTGGGCGACACCCTTTACCTGTGTACCGCTCACCAGCGCCTGTTTGCGCTTGAT
GCCGCCAGCGGCAAAGAGAAATGGCATTACGATCCTGAGCTGAAAACCAACGAGTCTTTC
CAGCACGTAACCTGCCGTGGTGTCTCTTATCATGAAGCCAAAGCAGAAACCGCTTCGCCG
GAAGTGATGGCGGATTGCCCGCGTCGTATCATTCTTCCGGTCAATGATGGTCGACTGATT
GCGATTAACGCTGAAAACGGCAAACTGTGCGAAACCTTCGCCAATAAAGGCGTGCTCAAT
CTGCAAAGCAATATGCCAGACACCAAACCGGGTCTGTATGAACCGACTTCGCCACCGATT
ATCACCGATAAAACCATCGTGATGGCCGGTTCAGTTACCGATAACTTCTCAACCCGCGAA
ACGTCTGGCGTGATCCGTGGTTTTGATGTCAACACCGGGGAGCTGCTGTGGGCTTTTGAT
CCCGGCGCGAAAGATCCGAACGCAATCCCGTCTGACGAACACACCTTTACCTTTAACTCG
CCAAACTCCTGGGCACCAGCGGCCTATGACGCGAAGCTGGATCTGGTCTATCTGCCGATG
GGCGTGACCACGCCGGATATCTGGGGCGGTAACCGCACACCGGAACAGGAACGTTATGCC
AGCTCGATTCTGGCGCTGAATGCCACTACCGGGAAACTGGCGTGGAGCTACCAGACCGTT
CACCACGACCTGTGGGACATGGATCTTCCGGCACAGCCGACGCTGGCGGACATCACCGTT
AATGGTCAGAAAGTGCCAGTTATTTACGCTCCGGCGAAAACCGGCAACATTTTTGTGCTC
GATCGTCGTAATGGCGAACTGGTGGTTCCGGCACCGGAAAAACCGGTTCCCCAAGGTGCA
GCGAAAGGCGATTACGTAACCCCAACTCAACCGTTTTCTGAACTGAGCTTCCGTCCGACG
AAAGATTTGAGCGGTGCGGATATGTGGGGAGCCACCATGTTTGACCAACTGGTGTGCCGC
GTGATGTTCCACCAGATGCGCTATGAAGGCATTTTCACCCCGCCATCTGAACAGGGTACG
CTGGTCTTCCCGGGTAACCTGGGGATGTTCGAATGGGGCGGGATTTCCGTTGATCCAAAT
CGTGAAGTGGCGATTGCCAACCCAATGGCACTGCCGTTTGTTTCGAAACTGATCCCGCGT
GGTCCTGGCAACCCGATGGAGCAGCCGAAAGATGCCAAAGGCACGGGTACGGAATCCGGC
ATTCAGCCACAGTACGGTGTACCGTATGGTGTCACGCTCAACCCGTTCCTCTCACCATTT
GGTCTGCCATGTAAACAGCCAGCATGGGGTTATATCTCGGCGCTGGATCTGAAAACTAAT
GAAGTGGTGTGGAAGAAACGTATTGGTACGCCGCAGGACAGTATGCCGTTCCCGATGCCG
GTTCCGGTGCCGTTCAATATGGGTATGCCGATGCTGGGCGGGCCAATCTCCACGGCGGGT
AACGTGCTGTTTATCGCCGCTACGGCAGATAACTACCTGCGCGCTTACAACATGAGCAAC
GGTGAAAAACTGTGGCAGGGTCGTTTACCAGCGGGTGGTCAGGCTACGCCAATGACCTAT
GAAGTGAATGGTAAGCAGTATGTGGTGATCTCCGCAGGCGGTCACGGTTCATTTGGTACG
AAGATGGGCGACTATATTGTGGCTTATGCGCTGCCGGATGATGTGAAGTAA

Protein Properties

Pfam Domain Function:

PQQ (PF01011 )
PQQ_C (PF10535 )
PQQ_N (PF10527 )

Protein Residues:

796

Protein Molecular Weight:

86747

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

11-37
41-58
63-81
96-110
119-141

Protein Sequence:

>Quinoprotein glucose dehydrogenase
MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPIAGLVMLGVAWMLWRS
KRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGIWLILPFVWRRLVIPASGA
VAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAISPVADQDWPAYGRNQEGQRF
SPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVTPIKVGDTLYLCTAHQRLFALD
AASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAETASPEVMADCPRRIILPVNDGRLI
AINAENGKLCETFANKGVLNLQSNMPDTKPGLYEPTSPPIITDKTIVMAGSVTDNFSTRE
TSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEHTFTFNSPNSWAPAAYDAKLDLVYLPM
GVTTPDIWGGNRTPEQERYASSILALNATTGKLAWSYQTVHHDLWDMDLPAQPTLADITV
NGQKVPVIYAPAKTGNIFVLDRRNGELVVPAPEKPVPQGAAKGDYVTPTQPFSELSFRPT
KDLSGADMWGATMFDQLVCRVMFHQMRYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPN
REVAIANPMALPFVSKLIPRGPGNPMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPF
GLPCKQPAWGYISALDLKTNEVVWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAG
NVLFIAATADNYLRAYNMSNGEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGT
KMGDYIVAYALPDDVK

References

External Links:

Resource	Link
Uniprot ID:	P15877
Uniprot Name:	DHG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21238978
Ecogene ID:	EG10369
Ecocyc:	EG10369
ColiBase:	b0124
Kegg Gene:	b0124
EchoBASE ID:	EB0364
CCDB:	DHG_ECOLI
BacMap:	16128117

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Cleton-Jansen, A. M., Goosen, N., Fayet, O., van de Putte, P. (1990). "Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase." J Bacteriol 172:6308-6315. Pubmed: 2228962
Cozier, G. E., Anthony, C. (1995). "Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobacterium extorquens." Biochem J 312 ( Pt 3):679-685. Pubmed: 8554505
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137
Yamada, M., Asaoka, S., Saier, M. H. Jr, Yamada, Y. (1993). "Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression." J Bacteriol 175:568-571. Pubmed: 8419307
Yamada, M., Sumi, K., Matsushita, K., Adachi, O., Yamada, Y. (1993). "Topological analysis of quinoprotein glucose dehydrogenase in Escherichia coli and its ubiquinone-binding site." J Biol Chem 268:12812-12817. Pubmed: 8509415