Identification
Name:2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Synonyms:Not Available
Gene Name:entA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:2,3-dihydro-2,3-dihydroxybenzoate + NAD(+) = 2,3-dihydroxybenzoate + NADH
Cellular Location:Not Available
SMPDB Pathways:
  • 2,3-dihydroxybenzoate biosynthesis PW000751
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
  • Enterobactin Biosynthesis PW002048
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
SMPDB Reactions:
1.02,3-dihydroxy-2,3-dihydrobenzoate+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-dihydroxy-2,3-dihydrobenzoate + 1.0NAD → 1.02,3-Dihydroxybenzoic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.02,3-dihydroxy-2,3-dihydrobenzoate+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.02,3-dihydroxy-2,3-dihydrobenzoate + 1.0NAD → 1.02-Pyrocatechuic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB20009(2S,3S)-2,3-Dihydro-2,3-dihydroxybenzoateMetaboCard
ECMDB040552,3-Dihydro-2,3-dihydroxybenzoic acidMetaboCard
ECMDB241842,3-Dihydroxybenzoic acidMetaboCard
ECMDB003972-Pyrocatechuic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
GO Classification:
Function
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity
binding
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
Process
cellular aromatic compound metabolic process
cellular metabolic process
enterobactin biosynthetic process
enterobactin metabolic process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0596
Gene OrientationClockwise
Centisome Percentage:13.53
Left Sequence End627774
Right Sequence End628520
Gene Sequence:
>747 bp
ATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCC
ACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACT
CAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCG
CAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCG
GGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTT
GCGGTTAACGTCGGCGGTGCGTTTAACCTGTTCCAGCAAACCATGAACCAGTTTCGCCGT
CAGCGGGGCGGGGCGATTGTCACTGTGGCGTCCGACGCCGCGCACACGCCGCGTATTGGC
ATGAGTGCTTATGGCGCATCGAAAGCGGCGCTGAAAAGCCTGGCGTTGAGCGTCGGGCTG
GAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGAT
ATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGC
GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAAC
ACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTC
GATGGCGGCTCAACGCTGGGGGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:248
Protein Molecular Weight:26250
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQYPFATEVMDVADAAQVA
QVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAVNVGGAFNLFQQTMNQFRR
QRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGLELAGSGVRCNVVSPGSTDTD
MQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEIANTILFLASDLASHITLQDIVV
DGGSTLGA
References
External Links:
ResourceLink
Uniprot ID:P15047
Uniprot Name:ENTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674717
Ecogene ID:EG10259
Ecocyc:EG10259
ColiBase:b0596
Kegg Gene:b0596
EchoBASE ID:EB0255
CCDB:ENTA_ECOLI
BacMap:16128579
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, J., Duncan, K., Walsh, C. T. (1989). "Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." J Bacteriol 171:791-798. Pubmed: 2521622
  • Nahlik, M. S., Brickman, T. J., Ozenberger, B. A., McIntosh, M. A. (1989). "Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA." J Bacteriol 171:784-790. Pubmed: 2521621