ECMDB: D-tagatose-1,6-bisphosphate aldolase subunit gatZ (P0C8J8)

Identification

Name:

D-tagatose-1,6-bisphosphate aldolase subunit gatZ

Synonyms:

Not Available

Gene Name:

gatZ

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Component of the tagatose-1,6-bisphosphate aldolase gatYZ that is required for full activity and stability of the Y subunit. Could have a chaperone-like function for the proper and stable folding of gatY. When expressed alone, gatZ does not show any aldolase activity. Is involved in the catabolism of galactitol

Cellular Location:

Not Available

SMPDB Pathways:

Galactitol and galactonate degradation PW000820
Galactose metabolism PW000821

KEGG Pathways:

Galactose metabolism ec00052

KEGG Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0D-Tagatose 6-phosphate → 1.0ADP + 1.0Hydrogen ion + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard

1.0

↔

1.0

1.0D-Tagatose 6-phosphate + 1.0Adenosine triphosphate ↔ 1.0D-Tagatose 1,6-bisphosphate + 1.0ADP
ReactionCard

SMPDB Reactions:

1.0D-tagatofuranose 1,6-bisphosphate

→

1.0

1.0D-Glyceraldehyde 3-phosphate

1.0

1.0D-tagatofuranose 1,6-bisphosphate → 1.0Dihydroxyacetone phosphate + 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0D-Tagatose 6-phosphate → 1.0ADP + 1.0Hydrogen ion + 1.0D-Tagatose 1,6-bisphosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB01112	D-Glyceraldehyde 3-phosphate	MetaboCard
ECMDB06872	D-Tagatose 1,6-bisphosphate	MetaboCard
ECMDB06873	D-Tagatose 6-phosphate	MetaboCard
ECMDB01473	Dihydroxyacetone phosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
catalytic activity
kinase activity
tagatose-6-phosphate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
alditol metabolic process
galactitol metabolic process
hexitol metabolic process
metabolic process
polyol metabolic process
small molecule metabolic process

Gene Properties

Blattner:

b2095

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>1263 bp
ATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTT
GCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGC
TGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAATCCGTGGTCAAGTGC
GTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT
CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCT
GAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGT
GTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAG
GAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTG
ATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCGGTTGAACGTTGTGGGCTG
AAAGTTGACCAACTGATATTTGCCGGACTGGCATCAAGTTATTCGGTATTGACGGAAGAT
GAACGTGAACTGGGTGTCTGCGTCGTCGATATCGGTGGTGGTACAATGGATATCGCCGTT
TATACCGGTGGGGCATTGCGCCACACTAAGGTAATTCCTTATGCTGGCAATGTCGTGACC
AGTGATATCGCTTACGCCTTTGGCACGCCGCCAAGCGACGCCGAAGCGATTAAAGTTCGC
CACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTA
GGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGCCGCGC
TATACCGAGCTGCTCAACCTGGTCAACGAAGAGATATTGCAGTTGCAGGAAAAGCTTCGC
CAACAAGGGGTTAAACATCACCTGGCGGCAGGCATTGTATTAACCGGTGGCGCAGCGCAG
ATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCG
CCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATTATTCGACGGCGGTG
GGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGT
GTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGAAAAGAGTTT
TAA

Protein Properties

Pfam Domain Function:

Tagatose_6_P_K (PF08013 )

Protein Residues:

420

Protein Molecular Weight:

47108

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>D-tagatose-1,6-bisphosphate aldolase subunit gatZ
MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIEATSNQVNQFGGYTGMT
PADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAAMEKSVELVKEYVRAGFSK
IHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQREQLSYVIGTEVPVPGGEAS
AIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIAIVVQPGVEFDHSNIIHYQPQEA
QPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFAILKVGPALTFALREAIFALAQIEQ
ELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGFNDSLLDIRYSLSDRIRYYWPHSRIKN
SVETMMVNLEGVDIPLGMISQYLPKQFERIQSGELSAIPHQLIMDKIYDVLRAYRYGCAE

References

External Links:

Resource	Link
Uniprot ID:	P0C8J8
Uniprot Name:	GATZ_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321975
ColiBase:	b2095
Kegg Gene:	b2095
EchoBASE ID:	EB2317
CCDB:	GATZ_ECOLI
BacMap:	16130033

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Brinkkotter, A., Shakeri-Garakani, A., Lengeler, J. W. (2002). "Two class II D-tagatose-bisphosphate aldolases from enteric bacteria." Arch Microbiol 177:410-419. Pubmed: 11976750
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
Nobelmann, B., Lengeler, J. W. (1996). "Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism." J Bacteriol 178:6790-6795. Pubmed: 8955298