Nucleoside permease nupC (P0AFF2)

Identification
Name:Nucleoside permease nupC
Synonyms:
  • Nucleoside-transport system protein nupC
Gene Name:nupC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleoside:sodium symporter activity
Specific Function:Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • adenine and adenosine salvage III PW002072
  • pyrimidine deoxyribonucleosides degradation PW002063
  • salvage pathways of pyrimidine deoxyribonucleotides PW002061
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00050AdenosineMetaboCard
ECMDB00089CytidineMetaboCard
ECMDB00101DeoxyadenosineMetaboCard
ECMDB00014DeoxycytidineMetaboCard
ECMDB00071DeoxyinosineMetaboCard
ECMDB00012DeoxyuridineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00195InosineMetaboCard
ECMDB21389RibothymidineMetaboCard
ECMDB00273ThymidineMetaboCard
ECMDB00296UridineMetaboCard
GO Classification:
Component
cell part
membrane
Function
nucleobase, nucleoside, nucleotide and nucleic acid transmembrane transporter activity
nucleoside transmembrane transporter activity
nucleoside:sodium symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b2393
Gene OrientationClockwise
Centisome Percentage:54.12
Left Sequence End2511064
Right Sequence End2512266
Gene Sequence:
>1203 bp
ATGACGCAATACTCCTCTCTCCTTCGTGGTCTTGCAGCGGGTTCTGCATTTTTATTCCTT
TTTGCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGT
GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAG
AAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTT
AAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACG
GGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG
GTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTG
GCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAA
AAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAG
TTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAA
GAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGT
AACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCA
GGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTA
GTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGG
GGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAG
CGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTG
ACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAG
CTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTAACGGT
AAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCTTT
GGTCGGGTGTGGGATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:400
Protein Molecular Weight:43475
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 4-24
  • 37-57
  • 88-108
  • 169-189
  • 193-213
  • 251-271
  • 282-302
  • 342-362
  • 379-399
Protein Sequence:
>Nucleoside permease nupC
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMG
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
References
External Links:
ResourceLink
Uniprot ID:P0AFF2
Uniprot Name:NUPC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651381
Ecogene ID:EG11971
Ecocyc:EG11971
ColiBase:b2393
Kegg Gene:b2393
EchoBASE ID:EB1914
CCDB:NUPC_ECOLI
BacMap:16130325
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Craig, J. E., Zhang, Y., Gallagher, M. P. (1994). "Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186." Mol Microbiol 11:1159-1168. Pubmed: 8022285
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837