Arginine N-succinyltransferase (P0AE37)

Identification
Name:Arginine N-succinyltransferase
Synonyms:
  • AST
  • AOST
Gene Name:astA
Enzyme Class:
Biological Properties
General Function:Involved in arginine N-succinyltransferase activity
Specific Function:Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Arginine and proline metabolism ec00330
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Arginine + 1.0Succinyl-CoA ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0N2-Succinyl-L-arginine
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Succinyl-CoA + 1.0L-Arginine ↔ 1.0Coenzyme A + 1.0N2-Succinyl-L-arginine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Succinyl-CoA+1.0Thumb → 1.0Thumb+1.0Thumb+1.0 N2-succinyl-L-arginine+1.0Thumb
1.0L-Arginine + 1.0Succinyl-CoA + 1.0Succinyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0 N2-succinyl-L-arginine + 1.0 N2-succinyl-L-arginine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Arginine + 1.0Succinyl-CoA ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0N2-Succinyl-L-arginine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Arginine + 1.0Succinyl-CoA → 1.0Hydrogen ion + 1.0N2-Succinyl-L-arginine + 1.0Coenzyme A
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24190 N2-succinyl-L-arginineMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00517L-ArginineMetaboCard
ECMDB20179N2-Succinyl-L-arginineMetaboCard
ECMDB01022Succinyl-CoAMetaboCard
GO Classification:
Function
acyltransferase activity
arginine N-succinyltransferase activity
catalytic activity
N-acyltransferase activity
N-succinyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
arginine catabolic process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process
Gene Properties
Blattner:b1747
Gene OrientationCounterclockwise
Centisome Percentage:39.39
Left Sequence End1827755
Right Sequence End1828789
Gene Sequence:
>1035 bp
ATGGCAAAGCCGATCATCACGCTCAATGGCCTAAAAATCGTCATTATGTTGGGAATGCTG
GTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCA
TTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCG
CGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTA
GCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCT
ATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCA
GTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTTA
TTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTT
ATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTT
GAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAA
ACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT
CGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGGT
TCAGTCCTCGCGGCAATCCCCCCTATCGCTCAGGTACTGGTGTTTAATGGCTTCTACGAA
GCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAG
CCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATT
TTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATT
GTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGAT
CTCAATAAAGAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:344
Protein Molecular Weight:38456
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Arginine N-succinyltransferase
MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAIKTWQGELPKSEQGYVF
VLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELNVYNALPTLFLSNDHTGSS
ELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVVAEMRGVIDEHGYSPFWQSLG
KRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLSQEAQDVIGQVHPQTAPARAVLE
KEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVEVAEGQPAQGDFPACLVANENYHHF
RVVLVRTDPATERLILTAAQLDALKCHAGDRVRLVRLCAEEKTA
References
External Links:
ResourceLink
Uniprot ID:P0AE37
Uniprot Name:ASTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742635
Ecogene ID:EG13998
Ecocyc:EG13998
ColiBase:b1747
Kegg Gene:b1747
EchoBASE ID:EB3754
CCDB:ASTA_ECOLI
BacMap:16129701
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kiupakis, A. K., Reitzer, L. (2002). "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli." J Bacteriol 184:2940-2950. Pubmed: 12003934
  • Schneider, B. L., Kiupakis, A. K., Reitzer, L. J. (1998). "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli." J Bacteriol 180:4278-4286. Pubmed: 9696779
  • Shirai, H., Mizuguchi, K. (2003). "Prediction of the structure and function of AstA and AstB, the first two enzymes of the arginine succinyltransferase pathway of arginine catabolism." FEBS Lett 555:505-510. Pubmed: 14675764