Identification
Name:Leu/Ile/Val-binding protein
Synonyms:
  • LIV-BP
Gene Name:livJ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in amino acid transport
Specific Function:This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Leucine + 1.0Adenosine triphosphate + 1.0Water → 1.0L-Leucine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
1.0L-Valine+1.0Thumb+1.0Thumb+1.0Thumb1.0L-Valine+1.0Adenosine diphosphate+1.0Pyrophosphate+1.0Thumb+1.0Thumb
1.0L-Valine + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Valine → 1.0L-Valine + 1.0Adenosine diphosphate + 1.0Pyrophosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
1.0L-Isoleucine+1.0Thumb+1.0Thumb+1.0Thumb1.0L-Isoleucine+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Isoleucine + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Isoleucine → 1.0L-Isoleucine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00161L-AlanineMetaboCard
ECMDB00172L-IsoleucineMetaboCard
ECMDB00687L-LeucineMetaboCard
ECMDB00167L-ThreonineMetaboCard
ECMDB00883L-ValineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Process
amine transport
amino acid transport
establishment of localization
transport
Gene Properties
Blattner:b3460
Gene OrientationCounterclockwise
Centisome Percentage:77.52
Left Sequence End3596578
Right Sequence End3597681
Gene Sequence:
>1104 bp
ATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCG
CGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCC
GCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTG
GGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAG
AGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGCATTCACGTCGGGCAA
ATGCTGCTGACCCGTGCTGATATGGAAGACCGTGAACGCTTCCTGAACGCCCGCGACACC
CTGCGAGCGTTGCTCGATAACAATATCGTTCCGGTAATCAATGAGAACGATGCTGTCGCT
ACGGCAGAGATTAAGGTCGGCGATAACGATAACCTTTCTGCGCTGGCGGCGATTCTTGCG
GGTGCCGATAAACTGTTGCTGCTGACCGATCAAAAAGGTTTGTATACCGCTGACCCGCGC
AGCAATCCGCAGGCAGAACTGATTAAAGATGTTTACGGCATTGATGACGCACTGCGCGCG
ATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCC
GCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGC
GTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACT
CCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTA
GATGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATT
AAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGC
CGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGA
CACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCAC
CGTGATGACATGATTACCCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:367
Protein Molecular Weight:39076
Protein Theoretical pI:5
PDB File:2LIV
Signaling Regions:
  • 1-23
Transmembrane Regions:
  • None
Protein Sequence:
>Leu/Ile/Val-binding protein
MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQEFTGAEQAVADINAKG
GIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMI
TPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVKPQRIAIVHDKQQYGEGLARA
VQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAG
LKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFV
WTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLKGFEFGVFDWHAN
GTATDAK
References
External Links:
ResourceLink
Uniprot ID:P0AD96
Uniprot Name:LIVJ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674394
PDB ID:2LIV
Ecogene ID:EG10539
Ecocyc:EG10539
ColiBase:b3460
Kegg Gene:b3460
EchoBASE ID:EB0534
CCDB:LIVJ_ECOLI
BacMap:49176358
General Reference:
  • Adams, M. D., Wagner, L. M., Graddis, T. J., Landick, R., Antonucci, T. K., Gibson, A. L., Oxender, D. L. (1990). "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." J Biol Chem 265:11436-11443. Pubmed: 2195019
  • Antonucci, T. K., Landick, R., Oxender, D. L. (1985). "The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function." J Cell Biochem 29:209-216. Pubmed: 4077929
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Landick, R., Oxender, D. L. (1985). "The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport." J Biol Chem 260:8257-8261. Pubmed: 3891753
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Ovchinnikov, Y. A., Aldanova, N. A., Grinkevich, V. A., Arzamazova, N. M., Moroz, I. N. (1977). "The primary structure of a Leu, Ile and Val (LIV)-binding protein from Escherichia coli." FEBS Lett 78:313-316. Pubmed: 328304
  • Sack, J. S., Saper, M. A., Quiocho, F. A. (1989). "Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine." J Mol Biol 206:171-191. Pubmed: 2649682
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620