Hydroxyacylglutathione hydrolase (P0AC84)

Identification
Name:Hydroxyacylglutathione hydrolase
Synonyms:
  • Glyoxalase II
  • Glx II
Gene Name:gloB
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0S-Lactoylglutathione → 1.0Glutathione + 1.0Hydrogen ion + 1.0D-Lactic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0S-Lactoylglutathione + 1.0Water ↔ 1.0Glutathione + 1.0D-Lactic acid
ReactionCard
1.0S-(2-Hydroxyacyl)glutathione+1.0Thumb ↔ 1.0Thumb+1.02-Hydroxy carboxylate
1.0S-(2-Hydroxyacyl)glutathione + 1.0Water ↔ 1.0Glutathione + 1.02-Hydroxy carboxylate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Lactoylglutathione + 1.0Water → 1.0Glutathione + 1.0Hydrogen ion + 1.0L-Lactic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0S-Lactoylglutathione → 1.0Glutathione + 1.0Hydrogen ion + 1.0D-Lactic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0S-Lactoylglutathione + 1.0Water ↔ 1.0Glutathione + 1.0D-Lactic acid
ReactionCard
Complex Reactions:
1.0S-(2-hydroxyacyl)glutathione+1.0Thumb → 1.0Thumb+1.0a 2-hydroxy carboxylate
1.0S-(2-hydroxyacyl)glutathione + 1.0Water → 1.0Glutathione + 1.0a 2-hydroxy carboxylate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01311D-Lactic acidMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00190L-Lactic acidMetaboCard
ECMDB01066S-LactoylglutathioneMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on ester bonds
hydroxyacylglutathione hydrolase activity
ion binding
metal ion binding
thiolester hydrolase activity
transition metal ion binding
zinc ion binding
Gene Properties
Blattner:b0212
Gene OrientationCounterclockwise
Centisome Percentage:5.04
Left Sequence End234027
Right Sequence End234782
Gene Sequence:
>756 bp
ATGAATCTTAACAGTATTCCCGCCTTTGATGACAATTACATCTGGGTTTTGAATGATGAA
GCAGGTCGCTGCCTGATTGTCGATCCCGGAGACGCAGAGCCAGTATTAAACGCCATTGCC
GCCAATAACTGGCAACCGGAGGCCATATTTCTCACCCACCATCACCACGATCACGTTGGC
GGCGTAAAAGAACTGGTGGAAAAGTTTCCACAAATTGTGGTGTATGGTCCACAAGAGACA
CAAGATAAGGGAACAACACAGGTAGTCAAAGATGGCGAAACTGCCTTCGTTTTGGGGCAT
GAATTTAGTGTAATTGCTACGCCGGGTCACACTTTAGGACATATCTGTTACTTCAGTAAA
CCTTATCTATTTTGCGGCGACACACTGTTTTCTGGTGGGTGTGGTCGGTTGTTTGAAGGG
ACAGCATCACAAATGTATCAATCACTTAAAAAGTTAAGTGCGTTACCTGACGATACATTG
GTATGTTGTGCTCATGAATATACCTTATCAAATATGAAGTTTGCTTTGAGTATTCTTCCG
CACGATTTGTCCATAAATGATTATTATCGTAAAGTTAAGGAGTTACGGGCAAAAAATCAA
ATAACACTACCCGTAATTCTGAAAAATGAGCGGCAAATTAATGTTTTTTTAAGAACGGAA
GATATTGATTTAATTAATGTAATTAATGAAGAAACATTATTGCAACAACCTGAAGAGCGT
TTTGCATGGTTAAGGTCAAAGAAAGATAGGTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:251
Protein Molecular Weight:28434
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Hydroxyacylglutathione hydrolase
MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNWQPEAIFLTHHHHDHVG
GVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEFSVIATPGHTLGHICYFSK
PYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTLVCCAHEYTLSNMKFALSILP
HDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLRTEDIDLINVINEETLLQQPEER
FAWLRSKKDRF
References
External Links:
ResourceLink
Uniprot ID:P0AC84
Uniprot Name:GLO2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902949
Ecogene ID:EG13330
Ecocyc:EG13330
ColiBase:b0212
Kegg Gene:b0212
EchoBASE ID:EB3114
CCDB:GLO2_ECOLI
BacMap:16128199
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Young, J., Sukdeo, N., Honek, J. F. (2007). "Escherichia coli glyoxalase II is a binuclear zinc-dependent metalloenzyme." Arch Biochem Biophys 459:20-26. Pubmed: 17196158