ECMDB: Diheme cytochrome c napB (P0ABL3)

Identification

Name:

Diheme cytochrome c napB

Synonyms:

Not Available

Gene Name:

napB

Enzyme Class:

Not Available

Biological Properties

General Function:

Energy production and conversion

Specific Function:

Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme napC protein, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Cellular Location:

Periplasm

SMPDB Pathways:

Nitrogen metabolism PW000755
Selenium metabolism PW001894
nitrate reduction VIII PW002092

KEGG Pathways:

Nitrogen metabolism ec00910

KEGG Reactions:

1.0

1.0Acceptor

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0Nitrite + 1.0Acceptor + 1.0Water + 1.0Acceptor ↔ 1.0Nitrate + 1.0Reduced acceptor + 1.0Reduced acceptor
ReactionCard

SMPDB Reactions:

1.0Nitrate

1.0cytochrome c nitrite reductase

1.0

↔

1.0Nitrite

1.0cytochrome c nitrite reductase

1.0

1.0Nitrate + 1.0cytochrome c nitrite reductase + 1.0Nitrate ↔ 1.0Nitrite + 1.0cytochrome c nitrite reductase + 1.0Water + 1.0Nitrite
ReactionCard

1.0

2.0

2.0Electron

→

1.0

1.0Selenate + 2.0Hydrogen ion + 2.0Electron → 1.0Selenite + 1.0Water
ReactionCard

1.0

2.0

1.0a menaquinol

→

1.0

1.0a menaquinone

1.0Nitrate + 2.0Hydrogen ion + 1.0a menaquinol → 1.0Nitrite + 1.0Water + 1.0a menaquinone
ReactionCard

Complex Reactions:

2.0

1.0

→

1.0

2.0

2.0Hydrogen ion + 1.0Nitrate + 1.0Ubiquinol-8 → 1.0Water + 1.0Nitrite + 1.0Ubiquinone-8 + 2.0Hydrogen ion
ReactionCard

2.0

1.0

→

1.0

1.0Menaquinone 8

1.0

2.0

2.0Hydrogen ion + 1.0Menaquinol 8 + 1.0Nitrate → 1.0Water + 1.0Menaquinone 8 + 1.0Nitrite + 2.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0Ubiquinol-8 + 1.0Nitrate → 1.0Ubiquinone-8 + 1.0Water + 1.0Nitrite
ReactionCard

1.0

→

1.0Menaquinone 8

1.0

1.0Menaquinol 8 + 1.0Nitrate → 1.0Menaquinone 8 + 1.0Water + 1.0Nitrite
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21245	Menaquinol 8	MetaboCard
ECMDB02878	Nitrate	MetaboCard
ECMDB02786	Nitrite	MetaboCard
ECMDB23788	Selenate	MetaboCard
ECMDB21355	Selenite	MetaboCard
ECMDB01060	Ubiquinol-8	MetaboCard
ECMDB21296	Ubiquinone-8	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b2203

Gene Orientation

Counterclockwise

Centisome Percentage:

49.49

Left Sequence End

2296291

Right Sequence End

2296740

Gene Sequence:

>450 bp
ATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGAC
GATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGT
GATGCTTCTCTTTCTGAAGGGCTGGAGCAACACCTGTTGGGGCTGAAAGTGGGCGATAAA
ACCACCTTCTCGTTGGAGCCAGATGCGGCGTTTGGCGTGCCGTCACCGGACCTGATTCAG
TACTTCTCCCGCCGTGAATTTATGGATGCAGGCGAGCCAGAAATTGGCGCAATCATGCTT
TTTACCGCAATGGATGGCAGTGAGATGCCTGGCGTGATCCGCGAAATTAACGGCGACTCC
ATTACCGTTGATTTCAACCATCCGCTGGCCGGGCAGACCGTTCATTTTGATATTGAAGTG
CTGGAAATCGATCCGGCACTGGAGGCGTAA

Protein Properties

Pfam Domain Function:

NapB (PF03892 )

Protein Residues:

149

Protein Molecular Weight:

16297

Protein Theoretical pI:

Signaling Regions:

1-27

Transmembrane Regions:

None

Protein Sequence:

>Diheme cytochrome c napB
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQDRMPLNY
VNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRR
YFCLQCHVPQADTAPIVGNTFTPSKGYGK

References

External Links:

Resource	Link
Uniprot ID:	P0ABL3
Uniprot Name:	NAPB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321910
Ecogene ID:	EG12061
Ecocyc:	EG12061
ColiBase:	b2203
Kegg Gene:	b2203
EchoBASE ID:	EB1991
CCDB:	NAPB_ECOLI
BacMap:	90111403

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Iobbi-Nivol, C., Crooke, H., Griffiths, L., Grove, J., Hussain, H., Pommier, J., Mejean, V., Cole, J. A. (1994). "A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12." FEMS Microbiol Lett 119:89-94. Pubmed: 8039676
Thomas, G., Potter, L., Cole, J. A. (1999). "The periplasmic nitrate reductase from Escherichia coli: a heterodimeric molybdoprotein with a double-arginine signal sequence and an unusual leader peptide cleavage site." FEMS Microbiol Lett 174:167-171. Pubmed: 10234835