Gluconate 5-dehydrogenase (P0A9P9)

Identification
Name:Gluconate 5-dehydrogenase
Synonyms:
  • 5-keto-D-gluconate 5-reductase
Gene Name:idnO
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Catalyzes a reversible reduction of 5-ketoglutanate to form D-gluconate. Dependent on NADP, almost inactive with NAD
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Gluconic acid + 1.0NAD + 1.0NADP ↔ 1.05-Keto-D-gluconate + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.05-Keto-D-gluconate+1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.05-Keto-D-gluconate + 1.0Hydrogen ion + 1.0NADPH + 1.05-Keto-D-gluconate + 1.0NADPH → 1.0NADP + 1.0Gluconic acid
ReactionCard
EcoCyc Reactions:
1.0NAD(P)++1.0Thumb ↔ 1.0NAD(P)H+1.0Thumb+1.0Thumb
1.0NAD(P)+ + 1.0Gluconic acid ↔ 1.0NAD(P)H + 1.05-Keto-D-gluconate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Keto-D-gluconate + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0Gluconic acid + 1.0NADP
ReactionCard
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.0Gluconic acid + 1.0NAD(P)(+) → 1.05-Keto-D-gluconate + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB117315-Keto-D-gluconateMetaboCard
ECMDB00625Gluconic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
binding
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b4266
Gene OrientationCounterclockwise
Centisome Percentage:96.79
Left Sequence End4490610
Right Sequence End4491374
Gene Sequence:
>765 bp
ATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTG
GTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTG
CTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGG
CTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGT
TTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAA
ACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGC
CCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTT
ATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATT
CTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTG
CTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGC
AGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTG
ACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTG
ATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:254
Protein Molecular Weight:27563
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Gluconate 5-dehydrogenase
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQA
VAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTA
VFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAASKGAVKMLTRGMCVELARHN
IQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
GHLLFVDGGMLVAV
References
External Links:
ResourceLink
Uniprot ID:P0A9P9
Uniprot Name:IDNO_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674310
Ecogene ID:EG12540
Ecocyc:EG12540
ColiBase:b4266
Kegg Gene:b4266
EchoBASE ID:EB2429
CCDB:IDNO_ECOLI
BacMap:16132088
General Reference:
  • Bausch, C., Peekhaus, N., Utz, C., Blais, T., Murray, E., Lowary, T., Conway, T. (1998). "Sequence analysis of the GntII (subsidiary) system for gluconate metabolism reveals a novel pathway for L-idonic acid catabolism in Escherichia coli." J Bacteriol 180:3704-3710. Pubmed: 9658018
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553