ECMDB: Anaerobic ribonucleoside-triphosphate reductase-activating protein (P0A9N8)

Identification

Name:

Anaerobic ribonucleoside-triphosphate reductase-activating protein

Synonyms:

Class III anaerobic ribonucleotide reductase small component

Gene Name:

nrdG

Enzyme Class:

1.97.1.4

Biological Properties

General Function:

Involved in [formate-C-acetyltransferase]-activating enzyme activity

Specific Function:

Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

Cellular Location:

Cytoplasm

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

1.0

2.0Flavodoxin reduced

2.0

→

1.0

2.0flavodoxin semi oxidized

1.0

1.0Cytidine triphosphate + 2.0Flavodoxin reduced + 2.0Hydrogen ion → 1.0dCTP + 2.0flavodoxin semi oxidized + 1.0Water
ReactionCard

1.0

2.0Flavodoxin reduced

2.0

→

1.0

2.0flavodoxin semi oxidized

1.0

1.0Adenosine triphosphate + 2.0Flavodoxin reduced + 2.0Hydrogen ion → 1.0dATP + 2.0flavodoxin semi oxidized + 1.0Water
ReactionCard

2.0Flavodoxin reduced

1.0

2.0

→

1.0

2.0flavodoxin semi oxidized

1.0

2.0Flavodoxin reduced + 1.0Guanosine triphosphate + 2.0Hydrogen ion → 1.0dGTP + 2.0flavodoxin semi oxidized + 1.0Water
ReactionCard

2.0Flavodoxin reduced

2.0

1.0

→

1.0

2.0flavodoxin semi oxidized

1.0

2.0Flavodoxin reduced + 2.0Hydrogen ion + 1.0Uridine triphosphate → 1.0Deoxyuridine triphosphate + 2.0flavodoxin semi oxidized + 1.0Water
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21174	5'-Deoxyadenosine	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB00082	Cytidine triphosphate	MetaboCard
ECMDB01532	dATP	MetaboCard
ECMDB00998	dCTP	MetaboCard
ECMDB01191	Deoxyuridine triphosphate	MetaboCard
ECMDB01440	dGTP	MetaboCard
ECMDB01273	Guanosine triphosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00696	L-Methionine	MetaboCard
ECMDB01185	S-Adenosylmethionine	MetaboCard
ECMDB00285	Uridine triphosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Component
cell part
cytoplasm
intracellular part
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
[formate-C-acetyltransferase]-activating enzyme activity
Process
cellular metabolic process
metabolic process
oxidation reduction
oxygen and reactive oxygen species metabolic process

Gene Properties

Blattner:

b4237

Gene Orientation

Counterclockwise

Centisome Percentage:

96.08

Left Sequence End

4457923

Right Sequence End

4458387

Gene Sequence:

>465 bp
ATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTA
TTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTT
GCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCGCTGGAT
ACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGC
CTGAAAGATGAAGCTACCCGCTCGCGGCTGAGTGAGTATTTGCCGGAAGTCCGTAGTCGC
TTGCTGTTACTGTTTTCGCGTCAGGATGCTGCCGTACTGGCGACAGAAGAAGGCAAGAAA
AACCTGATTGCCGAGATTAAAACCACACTTTCCACCCCGCTTGTTGCCGGGCAACCGAAA
CAGGATGTCACCGACGTGCTGTATACCGCTTTTATTCTGCGATAA

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

154

Protein Molecular Weight:

17446

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Anaerobic ribonucleoside-triphosphate reductase-activating protein
MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSGQPFTKAMEDQIINDLN
DTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIWVWTGYKLDELNAAQMQVV
DLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR

References

External Links:

Resource	Link
Uniprot ID:	P0A9N8
Uniprot Name:	NRDG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1736610
Ecogene ID:	EG12523
Ecocyc:	EG12523
ColiBase:	b4237
Kegg Gene:	b4237
EchoBASE ID:	EB2414
CCDB:	NRDG_ECOLI
BacMap:	16132059

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Sun, X., Eliasson, R., Pontis, E., Andersson, J., Buist, G., Sjoberg, B. M., Reichard, P. (1995). "Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme." J Biol Chem 270:2443-2446. Pubmed: 7852304
Tamarit, J., Gerez, C., Meier, C., Mulliez, E., Trautwein, A., Fontecave, M. (2000). "The activating component of the anaerobic ribonucleotide reductase from Escherichia coli. An iron-sulfur center with only three cysteines." J Biol Chem 275:15669-15675. Pubmed: 10821845