ECMDB

Identification

Name:

Aerobic C4-dicarboxylate transport protein

Synonyms:

Not Available

Gene Name:

dctA

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in symporter activity

Specific Function:

Responsible for the aerobic transport of the dicarboxylates fumarate, L- and D-malate and to a lesser extent succinate, from the periplasm across the inner membrane

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Gluconeogenesis from L-malic acid PW000819
L-glutamate metabolism PW000789
Lysine biosynthesis PW000771
inner membrane transport PW000786

KEGG Pathways:

Lysine biosynthesis ec00300
Two-component system ec02020

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831

Metabolites:

ECMDB ID	Name	View
ECMDB20015	(R)-Malate	MetaboCard
ECMDB00176	Fumaric acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard
ECMDB00156	L-Malic acid	MetaboCard
ECMDB00226	Orotic acid	MetaboCard
ECMDB00254	Succinic acid	MetaboCard

GO Classification:

Component
cell part
membrane
Function
carboxylic acid transmembrane transporter activity
dicarboxylic acid transmembrane transporter activity
organic acid transmembrane transporter activity
sodium:dicarboxylate symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carboxylic acid transport
dicarboxylic acid transport
establishment of localization
organic acid transport
transport

Gene Properties

Blattner:

b3528

Gene Orientation

Counterclockwise

Centisome Percentage:

79.32

Left Sequence End

3680184

Right Sequence End

3681470

Gene Sequence:

>1287 bp
ATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACC
CAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTG
ACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCG
GCGTTTATTGGTGATGAAATCCCACAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTT
GCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCAC
GGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCAT
ATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCA
GTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGC
AAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTT
GCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAA
GAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTG
GCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAG
CTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAG
TCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCA
CGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCG
ATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATC
TGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATG
CGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCG
CTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCG
AAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTG
GCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTG
CGTAAATTGATGATGAATCATCAGTAA

Protein Properties

Pfam Domain Function:

SDF (PF00375 )

Protein Residues:

428

Protein Molecular Weight:

45436

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

5-27
47-64
77-99
141-163
184-206
219-241
326-348
352-374

Protein Sequence:

>Aerobic C4-dicarboxylate transport protein
MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKLIKMIIAPVIFCTVVTG
IAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPGAGMNVDPATLDAKAVAVY
ADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLFGFALHRLGSKGQLIFNVIES
FSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQLGQLIICFYITCILFVVLVLGS
IAKATGFSIFKFIRYIREELLIVLGTSSSESALPRMLDKMEKLGCRKSVVGLVIPTGYSF
NLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLIVLLLSSKGAAGVTGSGFIVLAATLSA
VGHLPVAGLALILGIDRFMSEARALTNLVGNGVATIVVAKWVKELDHKKLDDVLNNRAPD
GKTHELSS

References

External Links:

Resource	Link
Uniprot ID:	P0A830
Uniprot Name:	DCTA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321894
Ecogene ID:	EG20044
Ecocyc:	EG20044
ColiBase:	b3528
Kegg Gene:	b3528
EchoBASE ID:	EB4140
CCDB:	DCTA_ECOLI
BacMap:	16131400

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Davies, S. J., Golby, P., Omrani, D., Broad, S. A., Harrington, V. L., Guest, J. R., Kelly, D. J., Andrews, S. C. (1999). "Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli." J Bacteriol 181:5624-5635. Pubmed: 10482502
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Reed, J. L., Patel, T. R., Chen, K. H., Joyce, A. R., Applebee, M. K., Herring, C. D., Bui, O. T., Knight, E. M., Fong, S. S., Palsson, B. O. (2006). "Systems approach to refining genome annotation." Proc Natl Acad Sci U S A 103:17480-17484. Pubmed: 17088549
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620

Aerobic C4-dicarboxylate transport protein (P0A830)