ECMDB: Argininosuccinate synthase (P0A6E4)

Identification

Name:

Argininosuccinate synthase

Synonyms:

Citrulline--aspartate ligase

Gene Name:

argG

Enzyme Class:

6.3.4.5

Biological Properties

General Function:

Involved in argininosuccinate synthase activity

Specific Function:

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate

Cellular Location:

Cytoplasm (Probable)

SMPDB Pathways:

Aspartate metabolism PW000787
arginine metabolism PW000790

KEGG Pathways:

Alanine, aspartate and glutamate metabolism ec00250
Arginine and proline metabolism ec00330
Metabolic pathways eco01100

KEGG Reactions:

1.0

↔

1.0

1.0L-Aspartic acid + 1.0Adenosine triphosphate + 1.0Citrulline ↔ 1.0Adenosine monophosphate + 1.0Argininosuccinic acid + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Citrulline + 1.0L-Aspartic acid ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0Argininosuccinic acid
ReactionCard

SMPDB Reactions:

1.0

1.0L-Aspartic acid

1.0

→

1.0Pyrophosphate

1.0

1.0Adenosine triphosphate + 1.0Citrulline + 1.0L-Aspartic acid + 1.0L-Aspartic acid → 1.0Pyrophosphate + 1.0Adenosine monophosphate + 1.0Argininosuccinic acid
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01006	Argininosuccinic acid	MetaboCard
ECMDB00904	Citrulline	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
argininosuccinate synthase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
Process
arginine biosynthetic process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process

Gene Properties

Blattner:

b3172

Gene Orientation

Clockwise

Centisome Percentage:

71.48

Left Sequence End

3316659

Right Sequence End

3318002

Gene Sequence:

>1344 bp
GTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATG
CGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACATTTGCTGGCTGCGGGGAAGCCG
TTGCCGCGCGCTATCGAAGCCGGGCATTTACATTCTATGATCCTCTGGGGGCCGCCGGGT
ACCGGCAAAACAACTCTCGCTGAAGTGATTGCCCGCTATGCGAACGCTGATGTGGAACGT
ATTTCTGCCGTCACCTCTGGCGTGAAAGAGATTCGCGAGGCGATCGAGCGCGCCCGGCAA
AACCGCAATGCAGGTCGCCGCACTATTCTTTTTGTTGACGAAGTTCACCGTTTCAACAAA
AGCCAGCAGGATGCATTTCTGCCACATATTGAAGACGGCACCATCACTTTTATTGGCGCA
ACCACTGAAAACCCGTCGTTTGAGCTTAATTCGGCACTGCTTTCCCGTGCCCGTGTCTAT
CTGTTGAAATCCCTGAGTACAGAGGATATTGAGCAAGTACTAACTCAGGCGATGGAAGAC
AAAACCCGTGGCTATGGTGGTCAGGATATTGTTCTGCCAGATGAAACACGACGCGCCATT
GCTGAACTGGTGAATGGCGACGCGCGCCGGGCGTTAAATACGCTGGAAATGATGGCGGAT
ATGGCCGAAGTCGATGATAGCGGTAAGCGGGTCCTGAAGCCTGAATTACTGACCGAAATC
GCCGGTGAACGTAGCGCCCGCTTTGATAACAAAGGCGATCGCTTTTACGATCTGATTTCC
GCACTGCATAAGTCGGTACGTGGTAGCGCACCCGATGCGGCGCTGTACTGGTATGCGCGA
ATTATTACCGCTGGTGGCGATCCGTTATATGTCGCGCGTCGCTGTCTGGCGATTGCGTCT
GAAGACGTCGGTAATGCCGATCCACGGGCGATGCAGGTGGCAATTGCGGCCTGGGATTGC
TTTACTCGCGTTGGCCCGGCGGAAGGTGAACGCGCCATTGCTCAGGCGATTGTTTACCTG
GCCTGCGCGCCAAAAAGCAACGCTGTCTACACTGCGTTTAAAGCCGCGCTGGCCGATGCT
CGCGAACGCCCGGATTATGACGTGCCGGTTCATTTGCGTAATGCGCCGACGAAATTAATG
AAGGAAATGGGCTACGGGCAGGAATATCGTTACGCTCATGATGAAGCAAACGCTTATGCT
GCCGGTGAGGTTTACTTCCCGCCGGAAATAGCACAAACACGCTATTATTTCCCGACAAAC
AGGGGCCTTGAAGGCAAGATTGGCGAAAAGCTCGCCTGGCTGGCTGAACAGGATCAAAAT
AGCCCCATAAAACGCTACCGTTAA

Protein Properties

Pfam Domain Function:

Arginosuc_synth (PF00764 )

Protein Residues:

447

Protein Molecular Weight:

49898

Protein Theoretical pI:

PDB File:

1KP3

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Argininosuccinate synthase
MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEEDYDAIPRRA
MEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNTTPLGRAVTGTMLVAAMKE
DGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDTDFIDELGGRHEMSEFMIACG
FDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVNPIMGVKFWDESVKIPAEEVTVR
FEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALL
HIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQGRWFDSQALMLRDSLQRWVASQITGEV
TLELRRGNDYSILNTVSENLTYKPERLTMEKGDSVFSPDDRIGQLTMRNLDITDTREKLF
GYAKTGLLSSSAASGVPQVENLENKGQ

References

External Links:

Resource	Link
Uniprot ID:	P0A6E4
Uniprot Name:	ASSY_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062469
PDB ID:	1KP3
Ecogene ID:	EG10068
Ecocyc:	EG10068
ColiBase:	b3172
Kegg Gene:	b3172
EchoBASE ID:	EB0066
CCDB:	ASSY_ECOLI
BacMap:	16131063

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Lemke, C. T., Howell, P. L. (2001). "The 1.6 A crystal structure of E. coli argininosuccinate synthetase suggests a conformational change during catalysis." Structure 9:1153-1164. Pubmed: 11738042
Lemke, C. T., Howell, P. L. (2002). "Substrate induced conformational changes in argininosuccinate synthetase." J Biol Chem 277:13074-13081. Pubmed: 11809762
Lemke, C., Yeung, M., Howell, P. L. (1999). "Expression, purification, crystallization and preliminary X-ray analysis of Escherichia coli argininosuccinate synthetase." Acta Crystallogr D Biol Crystallogr 55:2028-2030. Pubmed: 10666579
Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
Van Vliet, F., Crabeel, M., Boyen, A., Tricot, C., Stalon, V., Falmagne, P., Nakamura, Y., Baumberg, S., Glansdorff, N. (1990). "Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals." Gene 95:99-104. Pubmed: 2123815