ECMDB

Identification

Name:

Glutamate synthase [NADPH] large chain

Synonyms:

Glutamate synthase subunit alpha
GLTS alpha chain
NADPH-GOGAT

Gene Name:

gltB

Enzyme Class:

1.4.1.13

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

2 L-glutamate + NADP(+) = L-glutamine + 2- oxoglutarate + NADPH

Cellular Location:

Not Available

SMPDB Pathways:

L-glutamate metabolism PW000789
Nitrogen metabolism PW000755

KEGG Pathways:

Alanine, aspartate and glutamate metabolism ec00250
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Nitrogen metabolism ec00910

KEGG Reactions:

1.0

→

2.0

1.0

1.0alpha-Ketoglutarate + 1.0L-Glutamine + 1.0Hydrogen ion + 1.0NADPH → 2.0L-Glutamate + 1.0NADP
ReactionCard

2.0

1.0

↔

1.0

2.0L-Glutamate + 1.0NAD ↔ 1.0L-Glutamine + 1.0alpha-Ketoglutarate + 1.0NADH + 1.0Hydrogen ion
ReactionCard

2.0

1.0

↔

1.0

2.0L-Glutamate + 1.0NADP ↔ 1.0L-Glutamine + 1.0alpha-Ketoglutarate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard

2.0

1.0

↔

1.0

2.0L-Glutamate + 1.0NADP + 1.0Ammonia + 1.0Water ↔ 1.0L-Glutamine + 1.0alpha-Ketoglutarate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard

SMPDB Reactions:

2.0L-Glutamic acid

1.0

2.0

→

1.0

1.0NADPH

1.0

2.0L-Glutamic acid + 1.0NADP + 2.0L-Glutamate → 1.0L-Glutamine + 1.0NADPH + 1.0Hydrogen ion + 1.0Oxoglutaric acid + 1.0NADPH
ReactionCard

1.0

1.0NADPH

1.0

→

2.0L-Glutamic acid

1.0

2.0

1.0L-Glutamine + 1.0Oxoglutaric acid + 1.0NADPH + 1.0Hydrogen ion + 1.0NADPH → 2.0L-Glutamic acid + 1.0NADP + 2.0L-Glutamate
ReactionCard

EcoCyc Reactions:

2.0

1.0

→

1.0

2.0L-Glutamate + 1.0NADP → 1.0L-Glutamine + 1.0Oxoglutaric acid + 1.0NADPH
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB02812	alpha-Ketoglutarate	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00641	L-Glutamine	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard
ECMDB04123	Oxoglutaric acid	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
glutamate synthase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH2 group of donors
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamate biosynthetic process
glutamate metabolic process
glutamine family amino acid metabolic process
metabolic process
nitrogen compound metabolic process
oxidation reduction

Gene Properties

Blattner:

b3212

Gene Orientation

Clockwise

Centisome Percentage:

72.26

Left Sequence End

3352747

Right Sequence End

3357207

Gene Sequence:

>4554 bp
ATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTG
GGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGG
GATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTG
CGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGT
AAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTT
GCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAAT
AAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAA
ACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATC
GCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGT
CCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAA
GCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGTCTG
TGTATGCCGACGGATCTGCCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCG
GCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCG
CAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAA
TGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCC
GCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTG
CTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGG
CAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCAT
ATGGAGCCGTGGGATGGCCCGGCGGGCATCGTGATGTCCGACGGTCGTTTTGCCGCCTGT
AACCTCGACCGTAACGGTCTGCGTCCGGCGCGCTACGTCATCACCAAAGATAAGCTCATC
ACCTGCGCCTCTGAAGTCGGTATCTGGGATTACCAGCCTGACGAAGTGGTCGAAAAAGGC
CGCGTCGGGCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCG
GCAGAAACCGATGACGATCTGAAAAGCCGCCATCCATATAAAGAGTGGATGGAGAAAAAC
GTCCGCCGACTGGTACCGTTTGAAGATCTGCCCGATGAAGAAGTGGGTAGCCGCGAACTG
GACGACGACACGCTTGCCAGCTACCAGAAACAGTTTAACTACAGCGCGGAAGAGCTGGAC
TCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGAT
ACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAG
TTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTC
GCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGT
TTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAA
GAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTC
GAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAAAAATGGTACGTAGCGGCACCGTG
CTGCTGGTGCTCTCCGACCGGAATATCGCTAAAGATCGCCTGCCGGTTCCAGCCCCGATG
GCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATC
ATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACTTCGCCGTGTTGCTGGGCTTCGGC
GCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCAT
GCGATTGCCAAAGATTATCGTACCGTGATGCTCAACTACCGTAACGGCATCAACAAAGGC
TTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAA
CTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTC
AGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAA
CGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGC
GGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGC
GGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACG
CTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAA
CCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGC
CCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGTAATTCGAACTCC
GGTGAAGGCGGCGAAGACCCGGCGCGCTACGGCACCAACAAAGTGTCGCGCATCAAGCAG
GTGGCTTCCGGTCGCTTTGGGGTTACTCCGGCGTATCTGGTCAATGCCGACGTCATTCAG
ATTAAAGTCGCCCAGGGCGCGAAGCCAGGCGAAGGCGGTCAGTTGCCGGGTGATAAAGTC
ACGCCTTACATCGCCAAACTGCGCTATTCGGTGCCCGGAGTGACGCTGATCTCCCCGCCG
CCGCACCACGATATCTACTCTATCGAGGACTTAGCGCAGCTCATTTTCGACCTCAAGCAG
GTTAACCCGAAAGCGATGATCTCCGTGAAGCTGGTTTCCGAACCGGGAGTAGGCACCATC
GCGACTGGCGTGGCAAAAGCTTATGCGGACTTGATCACCATCGCAGGCTATGACGGCGGC
ACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTT
GTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTC
GATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGC
TTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCAT
CTGAACAACTGCGCAACGGGTGTAGCAACTCAGGATGACAAACTGCGTAAGAACCACTAT
CACGGCCTGCCATTCAAGGTGACGAATTACTTTGAGTTTATCGCCCGTGAAACCCGCGAG
CTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTG
AAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAG
ACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAT
AACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAG
AGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCA
GGCTATATCGCCCAGACGCACGGCGATCAGGGGCTGGCAGCCGATCCTATCAAAGCGTAC
TTCAACGGCACCGCAGGCCAGAGCTTCGGCGTGTGGAACGCGGGCGGCGTGGAACTGTAC
CTGACCGGTGATGCCAACGACTATGTCGGTAAAGGCATGGCGGGCGGCTTAATCGCCATT
CGTCCTCCGGTTGGTTCCGCCTTCCGCAGCCATGAAGCAAGCATTATCGGCAACACCTGC
CTGTATGGCGCGACCGGTGGTCGTCTGTATGCCGCAGGCCGCGCGGGTGAACGTTTCGGC
GTGCGTAACTCCGGTGCTATCACCGTGGTAGAAGGCATTGGCGACAACGGTTGTGAATAT
ATGACGGGTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATG
ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCG
GAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGT
CTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAAC
TGGTCAACCTTCGCCACTAAATTTGCGCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCA
CTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAA

Protein Properties

Pfam Domain Function:

GATase_2 (PF00310 )
Glu_syn_central (PF04898 )
Glu_synthase (PF01645 )
GXGXG (PF01493 )

Protein Residues:

1517

Protein Molecular Weight:

166708

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Glutamate synthase [NADPH] large chain
MTRKPRRHALSVPVRSGSEVGFPQSLGEVHDMLYDKSLERDNCGFGLIAHIEGEPSHKVV
RTAIHALARMQHRGAILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLN
KDPELAAAARRIVEEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWR
PRDMERRLFIARRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLES
AICLFHQRFSTNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDA
APFVNETGSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMH
MEPWDGPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKG
RVGPGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSREL
DDDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQQ
FAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLTTMK
EEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLPVPAPM
AVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETLGRLVDTH
AIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDVVGLCFQGAV
SRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPDVVRTLQQAVQS
GEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFKRFDTAAMSIGALS
PEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGRFGVTPAYLVNADVIQ
IKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHHDIYSIEDLAQLIFDLKQ
VNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGTGASPLSSVKYAGCPWELGL
VETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAESFGFGTGPMVALGCKYLRICH
LNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARETRELMAQLGVTRLVDLIGRTDLL
KELDGFTAKQQKLALSKLLETAEPHPGKALYCTENNPPFDNGLLNAQLLQQAKPFVDERQ
SKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLAADPIKAYFNGTAGQSFGVWNAGGVELY
LTGDANDYVGKGMAGGLIAIRPPVGSAFRSHEASIIGNTCLYGATGGRLYAAGRAGERFG
VRNSGAITVVEGIGDNGCEYMTGGIVCILGKTGVNFGAGMTGGFAYVLDESGDFRKRVNP
ELVEVLSVDALAIHEEHLRGLITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKA
LLGHRSRSAAELRVQAQ

References

External Links:

Resource	Link
Uniprot ID:	P09831
Uniprot Name:	GLTB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85676006
Ecogene ID:	EG10403
Ecocyc:	EG10403
ColiBase:	b3212
Kegg Gene:	b3212
EchoBASE ID:	EB0398
CCDB:	GLTB_ECOLI

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Gosset, G., Merino, E., Recillas, F., Oliver, G., Becerril, B., Bolivar, F. (1989). "Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12." Protein Seq Data Anal 2:9-16. Pubmed: 2643092
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Oliver, G., Gosset, G., Sanchez-Pescador, R., Lozoya, E., Ku, L. M., Flores, N., Becerril, B., Valle, F., Bolivar, F. (1987). "Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12." Gene 60:1-11. Pubmed: 3326786
Velazquez, L., Camarena, L., Reyes, J. L., Bastarrachea, F. (1991). "Mutations affecting the Shine-Dalgarno sequences of the untranslated region of the Escherichia coli gltBDF operon." J Bacteriol 173:3261-3264. Pubmed: 1673677

Glutamate synthase [NADPH] large chain (P09831)