| Identification |
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| Name: | 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase |
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| Synonyms: | Not Available |
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| Gene Name: | cpdB |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Involved in hydrolase activity |
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| Specific Function: | This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate |
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| Cellular Location: | Periplasm |
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| SMPDB Pathways: | |
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| KEGG Pathways: | |
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| KEGG Reactions: | | | | | | | | | | | | | | | | | |
1.0 | + | 1.0 | ↔ | 1.0Nucleoside 3'-phosphate |
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1.03'-Ribonucleotide | + | 1.0 | ↔ | 1.0 | + | 1.0 |
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| EcoCyc Reactions: | |
1.0a nucleoside 3'-phosphate | + | 1.0 | → | 1.0a ribonucleoside | + | 1.0 |
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1.0a nucleoside 2',3'-cyclic phosphate | + | 1.0 | → | 1.0a nucleoside 3'-phosphate | + | 1.0 |
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| Complex Reactions: | | | | | | | | | |
1.0Nucleoside 2',3'-cyclic phosphate | + | 1.0 | → | 1.0nucleoside 3'-phosphate |
| 1.0Nucleoside 2',3'-cyclic phosphate + 1.0 Water → 1.0nucleoside 3'-phosphate ReactionCard | |
1.0A 3'-ribonucleotide | + | 1.0 | → | 1.0a ribonucleoside | + | 1.0 |
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| Metabolites: | |
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| GO Classification: | | Function |
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| 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity | | binding | | catalytic activity | | cation binding | | cyclic-nucleotide phosphodiesterase activity | | hydrolase activity | | hydrolase activity, acting on ester bonds | | ion binding | | metal ion binding | | nucleotide binding | | phosphoric diester hydrolase activity | | phosphoric ester hydrolase activity | | Process |
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| cellular nitrogen compound metabolic process | | metabolic process | | nitrogen compound metabolic process | | nucleobase, nucleoside and nucleotide metabolic process | | nucleobase, nucleoside, nucleotide and nucleic acid metabolic process | | nucleoside phosphate metabolic process | | nucleotide catabolic process | | nucleotide metabolic process |
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| Gene Properties |
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| Blattner: | b4213 |
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| Gene Orientation | Counterclockwise |
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| Centisome Percentage: | 95.54 |
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| Left Sequence End | 4432645 |
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| Right Sequence End | 4434588 |
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| Gene Sequence: | >1944 bp
ATGATTAAGTTTAGCGCAACGCTCCTGGCCACGCTGATTGCCGCCAGTGTGAATGCAGCG
ACGGTCGATCTGCGTATCATGGAAACCACTGATCTGCATAGCAACATGATGGATTTCGAT
TATTACAAAGACACCGCCACGGAAAAATTCGGACTGGTACGTACGGCAAGCCTGATTAAC
GATGCCCGCAATGAAGTGAAAAACAGCGTACTGGTTGATAACGGCGATTTGATTCAGGGG
AGTCCGCTGGCCGATTACATGTCGGCGAAAGGATTAAAAGCAGGTGATATTCACCCGGTC
TATAAGGCATTAAATACGCTGGACTATACCGTCGGAACGCTTGGCAACCACGAGTTTAAC
TACGGTCTGGATTACCTGAAAAATGCGCTGGCAGGAGCGAAATTCCCTTATGTAAATGCC
AACGTCATTGACGCCAGAACCAAACAGCCAATGTTTACACCGTATTTAATTAAAGACACC
GAAGTGGTCGATAAAGACGGAAAAAAACAGACGCTGAAGATTGGCTATATTGGCGTCGTG
CCACCACAAATCATGGGCTGGGATAAAGCTAATTTATCCGGGAAAGTGACGGTGAATGAT
ATTACCGAAACCGTGCGCAAATACGTGCCTGAAATGCGCGAGAAAGGTGCCGATGTTGTT
GTCGTTCTGGCACATTCCGGGCTATCTGCCGATCCGTATAAAGTGATGGCGGAAAACTCA
GTTTATTACCTCAGTGAAATTCCGGGCGTTAACGCCATTATGTTTGGCCATGCTCACGCC
GTTTTCCCAGGTAAAGATTTTGCTGATATCGAAGGGGCTGATATCGCCAAAGGCACGCTG
AATGGTGTTCCGGCGGTAATGCCAGGCATGTGGGGCGATCATCTTGGTGTGGTCGACTTA
CAACTCAGTAATGACAGCGGTAAATGGCAGGTGACGCAGGCGAAAGCGGAAGCACGACCG
ATTTACGACATCGCTAATAAAAAATCCCTCGCGGCGGAAGACAGCAAGCTGGTAGAAACA
CTCAAAGCCGATCACGATGCCACACGCCAGTTCGTCAGCAAGCCAATCGGTAAATCTGCC
GACAATATGTATAGCTATCTGGCGCTGGTGCAGGACGATCCGACCGTGCAGGTGGTGAAC
AACGCGCAAAAAGCGTATGTCGAGCATTACATTCAGGGCGATCCGGATCTGGCAAAACTG
CCGGTGCTTTCAGCTGCCGCACCGTTTAAAGTCGGTGGTCGCAAAAATGACCCGGCAAGC
TATGTGGAGGTGGAAAAAGGCCAGTTGACCTTCCGTAATGCCGCCGATCTTTATCTCTAT
CCCAATACGCTGATTGTGGTGAAAGCCAGCGGTAAAGAGGTGAAAGAGTGGCTGGAGTGC
TCCGCGGGACAGTTTAACCAGATTGATCCCAACAGCACGAAACCACAGTCACTCATCAAC
TGGGATGGTTTCCGCACTTATAACTTTGATGTTATTGATGGTGTGAATTATCAGATTGAT
GTTACCCAGCCCGCCCGTTATGACGGCGAGTGCCAGATGATTAATGCCAATGCGGAAAGG
ATTAAGAACCTGACCTTTAATGGCAAGCCGATTGATCCGAACGCCATGTTCCTGGTTGCC
ACCAATAACTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCT
TTTGCTTCACCGGATGAGAACCGCTCGGTGCTGGCAGCGTGGATTGCTGATGAGTCGAAA
CGTGCGGGGGAAATTCACCCGGCGGCAGATAACAACTGGCGTTTAGCACCGATAGCTGGC
GATAAGAAACTGGATATCCGTTTCGAAACCTCTCCGTCAGATAAAGCCGCAGCGTTTATT
AAAGAGAAAGGGCAGTATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATT
TATCAGGTGGATTTGAGTAAGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 647 |
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| Protein Molecular Weight: | 70832 |
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| Protein Theoretical pI: | 5 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIN
DARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFN
YGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVV
PPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDL
QLSNDSGKWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSA
DNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
YVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVA
TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAG
DKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
- Liu, J., Beacham, I. R. (1990). "Transcription and regulation of the cpdB gene in Escherichia coli K12 and Salmonella typhimurium LT2: evidence for modulation of constitutive promoters by cyclic AMP-CRP complex." Mol Gen Genet 222:161-165. Pubmed: 2172762
- Liu, J., Burns, D. M., Beacham, I. R. (1986). "Isolation and sequence analysis of the gene (cpdB) encoding periplasmic 2',3'-cyclic phosphodiesterase." J Bacteriol 165:1002-1010. Pubmed: 3005231
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