D-lactate dehydrogenase (P06149)

Identification
Name:D-lactate dehydrogenase
Synonyms:
  • Respiratory D-lactate dehydrogenase
Gene Name:dld
Enzyme Class:
Biological Properties
General Function:Involved in flavin adenine dinucleotide binding
Specific Function:First component of the membrane-bound D-lactate oxidase, which is believed to play an important role in the energization of the active transport of a variety of sugars and amino acids
Cellular Location:Cell membrane; Peripheral membrane protein; Cytoplasmic side
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Pyruvate metabolism ec00620
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0NADH + 1.0Pyruvic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+2.0Thumb+1.0an ubiquinol  → 1.0Thumb+1.0ubiquinone
1.0D-Lactic acid + 2.0Hydrogen ion + 1.0an ubiquinol  → 1.0Pyruvic acid + 1.0ubiquinone
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Lactic acid + 1.0Ubiquinone-6 → 1.0Pyruvic acid + 1.0Ubiquinol-6
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0NADH + 1.0Pyruvic acid
ReactionCard
1.0an electron-transfer-related quinone+1.0Thumb → 1.0an electron-transfer-related quinol+1.0Thumb
1.0an electron-transfer-related quinone + 1.0D-Lactic acid → 1.0an electron-transfer-related quinol + 1.0Pyruvic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0Ubiquinone-8 → 1.0Pyruvic acid + 1.0Ubiquinol-8
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0NAD → 1.0Pyruvic acid + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01311D-Lactic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00190L-Lactic acidMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB21460Ubiquinol-6MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB20620Ubiquinone-6MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
D-lactate dehydrogenase activity
FAD or FADH2 binding
lactate dehydrogenase activity
NAD or NADH binding
nucleoside binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
purine nucleoside binding
Process
carboxylic acid metabolic process
cellular metabolic process
establishment of localization
lactate metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
transmembrane transport
transport
Gene Properties
Blattner:b2133
Gene OrientationClockwise
Centisome Percentage:47.85
Left Sequence End2220207
Right Sequence End2221922
Gene Sequence:
>1716 bp
ATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTG
GGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGT
TCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGG
GTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGC
CTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGC
ACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCG
GGCACCACGCTCTATTCGCTGGAAAAAGCCCTCAAACCGCTGGGACGCGAACCGCACTCA
GTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGC
GGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAAT
GAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAG
CAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGT
CGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCC
GCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG
GCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGTTTTAT
ATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTC
GAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGCGGAAAAA
TACGGCAAAGACACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTC
TTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCAT
TTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGC
ATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGC
GTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTT
GTCTGTACGCCGGAGGAAGGCAGCAAAGCGTTTTTACACCGTTTCGCCGCTGCGGGCGCA
GCAATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATC
GCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAG
CTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATA
GTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAG
CGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACG
TTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGTAAA
ACCAGTAAACGGAAAAACTGGCAGGAAGTGGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:571
Protein Molecular Weight:64612
Protein Theoretical pI:7
PDB File:1F0X
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-lactate dehydrogenase
MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR
VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP
GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN
EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP
ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF
ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH
FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF
VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ
LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET
LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE
References
External Links:
ResourceLink
Uniprot ID:P06149
Uniprot Name:DLD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675247
PDB ID:1F0X
Ecogene ID:EG10231
Ecocyc:EG10231
ColiBase:b2133
Kegg Gene:b2133
EchoBASE ID:EB0227
CCDB:DLD_ECOLI
BacMap:16130071
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Campbell, H. D., Rogers, B. L., Young, I. G. (1984). "Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli." Eur J Biochem 144:367-373. Pubmed: 6386470
  • Dym, O., Pratt, E. A., Ho, C., Eisenberg, D. (2000). "The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme." Proc Natl Acad Sci U S A 97:9413-9418. Pubmed: 10944213
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Rule, G. S., Pratt, E. A., Chin, C. C., Wold, F., Ho, C. (1985). "Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli." J Bacteriol 161:1059-1068. Pubmed: 3882663