Dihydrodipicolinate reductase (P04036)

Identification
Name:Dihydrodipicolinate reductase
Synonyms:
  • DHPR
Gene Name:dapB
Enzyme Class:
Biological Properties
General Function:Involved in dihydrodipicolinate reductase activity
Specific Function:2,3,4,5-tetrahydrodipicolinate + NAD(P)(+) = 2,3-dihydrodipicolinate + NAD(P)H
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02,3-Dihydrodipicolinic acid + 1.0Hydrogen ion + 1.0NADPH → 1.0NADP + 1.0Tetrahydrodipicolinate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydrodipicolinate + 1.0NAD ↔ 1.02,3-Dihydrodipicolinic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydrodipicolinate + 1.0NADP ↔ 1.02,3-Dihydrodipicolinic acid + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydrodipicolinate + 1.0NAD + 1.0NADP + 1.0Water ↔ 1.0(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinate + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate+1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + 1.0Hydrogen ion + 1.0NADPH + 1.0(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinate + 1.0NADPH → 1.0Water + 1.0NADP + 1.0(S)-2,3,4,5-tetrahydrodipicolinate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23778(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinateMetaboCard
ECMDB24187(S)-2,3,4,5-tetrahydrodipicolinateMetaboCard
ECMDB040562,3-Dihydrodipicolinic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB12289TetrahydrodipicolinateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
dihydrodipicolinate reductase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
Process
aspartate family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
lysine biosynthetic process
lysine biosynthetic process via diaminopimelate
lysine metabolic process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0031
Gene OrientationClockwise
Centisome Percentage:0.61
Left Sequence End28374
Right Sequence End29195
Gene Sequence:
>822 bp
ATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAG
TTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAA
GGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT
ACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACC
CGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGATGGTG
ATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGAT
ATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTAAGCTGCTG
GAGAAAGCAGCCAAAGTGATGGGTGACTACACCGATATCGAAATTATTGAAGCACATCAT
AGACATAAAGTTGATGCGCCGTCAGGCACCGCACTGGCAATGGGAGAGGCGATCGCCCAC
GCCCTTGATAAAGATCTGAAAGATTGCGCGGTCTACAGTCGTGAAGGCCACACCGGTGAA
CGTGTGCCTGGCACCATTGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACAT
ACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGT
ATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGT
CTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:273
Protein Molecular Weight:28756
Protein Theoretical pI:6
PDB File:1DRW
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Dihydrodipicolinate reductase
MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSLLGSDAGELAGAGKTGV
TVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIGTTGFDEAGKQAIRDAAAD
IAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHHRHKVDAPSGTALAMGEAIAH
ALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVGEHTAMFADIGERLEITHKASSR
MTFANGAVRSALWLSGKESGLFDMRDVLDLNNL
References
External Links:
ResourceLink
Uniprot ID:P04036
Uniprot Name:DAPB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321913
PDB ID:1DRW
Ecogene ID:EG10206
Ecocyc:EG10206
ColiBase:b0031
Kegg Gene:b0031
EchoBASE ID:EB0202
CCDB:DAPB_ECOLI
BacMap:16128025
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bouvier, J., Patte, J. C., Stragier, P. (1984). "Multiple regulatory signals in the control region of the Escherichia coli carAB operon." Proc Natl Acad Sci U S A 81:4139-4143. Pubmed: 6377309
  • Bouvier, J., Richaud, C., Richaud, F., Patte, J. C., Stragier, P. (1984). "Nucleotide sequence and expression of the Escherichia coli dapB gene." J Biol Chem 259:14829-14834. Pubmed: 6094578
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Scapin, G., Blanchard, J. S., Sacchettini, J. C. (1995). "Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase." Biochemistry 34:3502-3512. Pubmed: 7893645
  • Scapin, G., Reddy, S. G., Zheng, R., Blanchard, J. S. (1997). "Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate." Biochemistry 36:15081-15088. Pubmed: 9398235
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901