Protein tonB (P02929)

Identification
Name:Protein tonB
Synonyms:Not Available
Gene Name:tonB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in iron ion transmembrane transporter activity
Specific Function:Interacts with outer membrane receptor proteins that carry out high-affinity binding and energy dependent uptake into the periplasmic space of specific substrates such as cobalamin, and various iron compounds (such as iron dicitrate, enterochelin, aerobactin, etc.). In the absence of tonB these receptors bind their substrates but do not carry out active transport. TonB also interacts with some colicins and is involved in the energy- dependent, irreversible steps of bacteriophages phi 80 and T1 infection. It could act to transduce energy from the cytoplasmic membrane to specific energy-requiring processes in the outer membrane, resulting in the release into the periplasm of ligands bound by these outer membrane proteins
Cellular Location:Cell inner membrane; Single-pass membrane protein; Periplasmic side
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB02086AdenosylcobalaminMetaboCard
ECMDB04040Cob(I)alaminMetaboCard
ECMDB06902CobinamideMetaboCard
ECMDB21213Fe(III)dicitrateMetaboCard
ECMDB21331ferric 2,3-dihydroxybenzoylserineMetaboCard
ECMDB20516Ferric coprogenMetaboCard
ECMDB21372Ferric enterobactinMetaboCard
ECMDB21218FerrichromeMetaboCard
ECMDB21333FerroxamineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
outer membrane-bounded periplasmic space
periplasmic space
Function
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
iron ion transmembrane transporter activity
protein transporter activity
substrate-specific transmembrane transporter activity
substrate-specific transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
iron ion transport
metal ion transport
protein transport
transition metal ion transport
transport
Gene Properties
Blattner:b1252
Gene OrientationClockwise
Centisome Percentage:28.22
Left Sequence End1309113
Right Sequence End1309832
Gene Sequence:
>720 bp
GTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGA
GACTCCGTGCTGCTACTGGAAAAACTCGGCTGTCGCGTAAATTTCCCGGAGAAACAGGGA
TGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAA
AATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGC
ACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATCACGT
GCCGCAAAGGTTGCCGCGCGTATGCAGGATCTCACCTCTTTTATTGTTAATAAATTAGGG
GTAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTG
GCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTG
GAGCTGTTGACCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTC
AAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTC
CGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTA
CAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:239
Protein Molecular Weight:26094
Protein Theoretical pI:10
PDB File:1XX3
Signaling Regions:
  • None
Transmembrane Regions:
  • 1-32
Protein Sequence:
>Protein tonB
MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVTPADLEPPQA
VQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESR
PASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKF
DVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ
References
External Links:
ResourceLink
Uniprot ID:P02929
Uniprot Name:TONB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674450
PDB ID:1XX3
Ecogene ID:EG11012
Ecocyc:EG11012
ColiBase:b1252
Kegg Gene:b1252
EchoBASE ID:EB1005
CCDB:TONB_ECOLI
BacMap:16129213
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chang, C., Mooser, A., Pluckthun, A., Wlodawer, A. (2001). "Crystal structure of the dimeric C-terminal domain of TonB reveals a novel fold." J Biol Chem 276:27535-27540. Pubmed: 11328822
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Karlsson, M., Hannavy, K., Higgins, C. F. (1993). "A sequence-specific function for the N-terminal signal-like sequence of the TonB protein." Mol Microbiol 8:379-388. Pubmed: 8316087
  • Postle, K., Good, R. F. (1983). "DNA sequence of the Escherichia coli tonB gene." Proc Natl Acad Sci U S A 80:5235-5239. Pubmed: 6310567
  • Roof, S. K., Allard, J. D., Bertrand, K. P., Postle, K. (1991). "Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions." J Bacteriol 173:5554-5557. Pubmed: 1885532