ECMDB: 3-phenylpropionate/cinnamic acid dioxygenase subunit beta (Q47140)

Identification

Name:

3-phenylpropionate/cinnamic acid dioxygenase subunit beta

Synonyms:

Digoxigenin subunit beta

Gene Name:

hcaF

Enzyme Class:

1.14.12.19

Biological Properties

General Function:

Involved in 3-phenylpropionate dioxygenase activity

Specific Function:

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively

Cellular Location:

Not Available

SMPDB Pathways:

2-Oxopent-4-enoate metabolism PW001890
2-Oxopent-4-enoate metabolism 2 PW002035
Phenylalanine metabolism PW000921

KEGG Pathways:

Microbial metabolism in diverse environments ec01120
Phenylalanine metabolism ec00360

KEGG Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0trans-Cinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

→

1.0

1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0NADH + 1.0Hydrogen ion + 1.0Oxygen + 1.0Hydrocinnamic acid ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD + 1.0Trans-2,3-Dihydroxycinnamate
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0Oxygen + 1.0NADH → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

↔

1.0

1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

1.0

→

1.0

1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Hydrogen ion + 1.0NADH + 1.0Oxygen + 1.0Hydrocinnamic acid → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21422	Cinnamic acid	MetaboCard
ECMDB20129	cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol	MetaboCard
ECMDB21320	Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol	MetaboCard
ECMDB20130	cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol	MetaboCard
ECMDB00764	Hydrocinnamic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00902	NAD	MetaboCard
ECMDB01487	NADH	MetaboCard
ECMDB04124	Oxygen	MetaboCard
ECMDB20197	Trans-2,3-Dihydroxycinnamate	MetaboCard
ECMDB00930	trans-Cinnamic acid	MetaboCard

GO Classification:

Function
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
oxidation reduction

Gene Properties

Blattner:

b2539

Gene Orientation

Clockwise

Centisome Percentage:

57.51

Left Sequence End

2668412

Right Sequence End

2668930

Gene Sequence:

>519 bp
ATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCG
TGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATG
GGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAC
TCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAA
GACATGGGTGTGCACGATCATGGCAGCATTGTCTGGGACGAATTTATTGGTATGTGGATC
ACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTC
CGTATTCTGGATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGC
GGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTAT
TTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAG

Protein Properties

Pfam Domain Function:

Ring_hydroxyl_B (PF00866 )

Protein Residues:

172

Protein Molecular Weight:

20579

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>3-phenylpropionate/cinnamic acid dioxygenase subunit beta
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQ
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF

References

External Links:

Resource	Link
Uniprot ID:	Q47140
Uniprot Name:	HCAF_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674558
Ecogene ID:	EG13457
Ecocyc:	EG13457
ColiBase:	b2539
Kegg Gene:	b2539
EchoBASE ID:	EB3230
CCDB:	HCAF_ECOLI
BacMap:	16130464

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Diaz, E., Ferrandez, A., Garcia, J. L. (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 180:2915-2923. Pubmed: 9603882
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837